Effects of X-Ray Radiation on Lipid Peroxidation and Antioxidant Systems in Rabbits Treated with Antioxidant Compounds

Abstract

The aim of this study was to investigate the effects of supplemental antioxidant vitamins and minerals on lipid peroxidation and on the antioxidant systems in rabbits exposed to X-rays. The rabbits were divided into two experimental groups and one control group, each group containing seven rabbits. The first group (VG) received daily oral doses of vitamin E (460 mg/kg live weight) and vitamin C (100 mg/kg live weight). The second group (MG) was fed a mineral-enriched diet that contained 60 mg manganese chloride, 40 mg zinc sulfate, and 5 mg copper sulfate per kilogram of feed. The third group served as controls and received only a standard diet. Blood samples were obtained before and after the supplementation with vitamins or minerals, as well as before and after irradiation with a total dose of 550-rad X-rays. The blood samples were analyzed for their content of malondialdehyde (MDA), plasma vitamins C and E, retinol, reduced glutathione (GSH), and glutathione peroxidase activity (GPx). After irradiation, the control group showed increased levels of MDA and activity of GPx (p<0.05), whereas the levels of GSH, vitamin C, and vitamin E were decreased. In the VG, the concentration of MDA was lower (p<0.05), and the concentration of GSH and vitamins C and E were higher (p<0.05) when compared to controls. In the MG, the concentrations of MDA, GSH, vitamin C, and retinol were not affected by the mineral administration and radiation. The level of vitamin E in the MG increased with mineral administration (p<0.05), but decreased after irradiation (p<0.05). For the control group, the level of GSH was higher than in the two experimental groups. After irradiation, the VG animals had vitamin E and C levels that were higher than in MG and control groups (p<0.05). The activity of GPx was not affected by vitamin or mineral supplementation or by irradiation. We conclude that the supplementation with antioxidant vitamins and minerals may serve to reinforce the antioxidant systems, thus having a protective effect against cell damage by X-rays.

Effect of X‐Radiation on Lipid Peroxidation and Antioxidant Systems in Rats Treated with Saponin‐containing Compounds

“…1). These results are consistent with earlier literature (4,5,37). Plasma MDA levels decreased in extract‐treated groups with increasing extract doses (25–50 mg kg −1 ).…”

“…Methanol extract of heat‐processed neoginseng attenuated lipid peroxidation in rats induced by ferric ion or ferric ion plus ascorbic acid (34), Vietnamese ginseng saponin has preventive effects on the psychological stress‐induced brain cell membrane damage (35) and bacoside A (triterpenoid saponin) administration improved the antioxidant status and maintained the levels of trace elements (36). A number of researchers have showed that irradiation increased the formation of MDA (4,5,33). According to the results of the present study, when rats were exposed to XR (5–10 Gy) of whole‐body irradiation, lipid peroxidation was found to be significantly increased in the blood with XR dose (Fig.…”

Effects of Vitamins C and E Combination on Element Levels in Blood of Smoker and Nonsmoker Radiology X-Ray Technicians

“…Sirmali et al [25] reported that serum zinc and copper levels in a rat hind limb model were increased by VCE supplementation. Deger et al [26] reported that blood antioxidant levels such as glutathione, vitamins C and E in rabbit were not affected by radiation and multimineral (manganese, zinc, and copper) supplementation, although their values were increased by VCE supplementation.…”

Role of germanium L-cysteine α-tocopherol complex as stimulator of some antioxidant defense systems in gamma-irradiated rats

“…Major biomarkers of oxidative damage to living cells are lipid peroxidation (LPx) products, DNA-hydroxylation and protein hydroxylation products (2). Efficient defense and repair mechanisms exist in living cells to protect against oxidant species (3). The antioxidative defense system is composed of methods to: transfer sensitive material to compartments better protected from the action of reactive species, complex transition metals, a potential source of electrons, thereby rendering them unreactive, inhibit vulnerable processes such as DNA replication, repair damaged molecules, initiate apoptosis, and, possibly most important considering the liability to internal and external modifying factors, activate antioxidant enzymes, and finally use a variety of direct free radical scavengers (4).…”