Effects of X-ray irradiation on haemocytes of <i>Procambarus clarkii</i> (Arthropoda: Decapoda) males

Giglio, Anita; Manfrin, Chiara; Zanetti, Marcus Antônio; Aquiloni, Laura; Simeon, Enrico; Bravin, M. K.; Battistella, Silvia; Giulianini, Piero Giulio

doi:10.1080/24750263.2017.1423119

Cited by 6 publications

(6 citation statements)

References 22 publications

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“…It has been successfully applied to P. clarkii where males were subjected to a radiation of 40 Gy (four times the lethal dose for a human), demonstrably reducing fertility by over 50% without compromising their vitality [133]. Crayfish that underwent X-ray exposure showed a significant decrease of about 80% of circulating haemocytes, thus this method affects important functions such as those of the immune system, in addition to altering the gonad tissue [134]. SMRT, in combination with intensive trapping, has proved to be particularly effective in a small lake (Casette lake, Pordenone, Friuli Venezia Giulia, Italy) with about 87% reduction of P. clarkii population after two years of activity [135].…”

Section: Sterile Male Release Technique (Smrt)mentioning

confidence: 99%

Detection and Control of Invasive Freshwater Crayfish: From Traditional to Innovative Methods

et al. 2019

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Invasive alien species are widespread in freshwater systems compared to terrestrial ecosystems. Among crustaceans, crayfish in particular have been widely introduced and are considered a major threat to freshwater ecosystem functioning. New emerging techniques for detecting and controlling invasive crayfish and protecting endangered native species are; thus, now highly desirable and several are under evaluation. Important innovations have been developed in recent years for detection of both invasive and native crayfish, mainly through eDNA, which allows for the detection of the target species even at low abundance levels and when not directly observable. Forecasting models have also moved towards the creation of realistic invasion scenarios, allowing effective management plans to be developed in advance of invasions. The importance of monitoring the spread and impacts of crayfish and pathogens in developing national data and research networks is emphasised; here “citizen science” can also play a role. Emerging techniques are still being considered in the field of invasive crayfish control. Although for decades the main traditional techniques to manage invasive crayfish were solely based on trapping, since 2010 biological, biocidal, autocidal controls and sexual attractants, monosex populations, RNA interference, the sterile male release technique and oral delivery have all also been investigated for crayfish control. In this review, ongoing methodologies applied to the detection and management of invasive crayfish are discussed, highlighting their benefits and limitations.

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Section: Sterile Male Release Technique (Smrt)mentioning

confidence: 99%

Detection and Control of Invasive Freshwater Crayfish: From Traditional to Innovative Methods

et al. 2019

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“…A strong reduction in circulating haemocytes was observed in P. clarkii after 20 days from X‐ray exposition of 40 Gy, 17 in Crangon crangon exposed to contaminated harbour dredgings, reared in mesocosms, 32 in crayfish haemocytes activated by lipopolysaccharides, 33 and correlated to the pesticide exposure with a dose close to LD 50 34…”

Section: Discussionmentioning

confidence: 97%

“…In a previous study, 16 extensive damage to the germinative tissues was observed in red swamp crayfish males that underwent 40 Gy of ionizing radiation after 30 days from the initial exposure without affecting crayfish vitality. In addition, short‐term X‐ray effects were also evaluated on circulating haemocytes of P. clarkii , after 20 days from irradiation of 40 Gy, leading a decrease of about 80% of circulating haemocytes in exposed animals and an increase in the percentage of semigranular and granular haemocytes 17 …”

Section: Introductionmentioning

confidence: 99%

Medium‐term feasibility of the management of the invasive crayfish Procambarus clarkii with the sterile males release technique

Manfrin

Giglio

Pallavicini

et al. 2021

Pest Management Science

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BACKGROUND The sterile male release technique (SMRT) is a useful method applied for controlling invasive and pest species. However, the use of X‐rays can lead to negative effects on the survival and health conditions of sterilized males. RESULTS This study was set up to evaluate the functional integrity of physiological, morphological and behavioural responses in males of the red swamp crayfish, Procambarus clarkii (Girard, 1852), exposed to a dose of 40 Gy of ionizing radiation. Concerning physiological responses, the results showed that the irradiation dose, tested at 5, 12, 28, 35, 65, 99, 132 and 193 days after treatment, has no effects on glycaemic and plasmatic total protein levels measured as biomarkers for general stress indexes. Nevertheless, the significant reduction of circulating haemocytes and the basal levels of phenoloxidase (PO) activities recorded in 40‐Gy irradiated crayfishes indicate that the exposure shrinks their capability to mount a rapid nonspecific response, and higher levels of plasmatic total PO activity indicate the ability to compensate and maintain an inducible response. Histological analyses performed at the end of the experiment showed no morphological damage in the testicular acini of irradiated males. Moreover, behavioural responses to two different water stimuli (vaporization and jet), measured at 15 and 45 days after the irradiation, were not modified in exposed crayfishes compared to the control group. CONCLUSIONS These results confirm the validity of SMRT on young males when the breeding season is less than 4 months but exposure to X‐ray should be repeated at mid‐breeding season when temperatures allow a longer breeding season. © 2021 Society of Chemical Industry

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“…Lack of acquired (specific) immune response in crayfish is due to the lack of myeloid lineage cells being able to produce immunoglobulins (specific antibodies) against specific pathogens in the event of FIGURE 1 | Schematic presentation of crayfish immune response. Presentation of the main constituents of crayfish immune response alongside the cooperative action of the cellular-mediated and humoral-mediated responses and their corresponding factors (4,5,14,17,18,26,(28)(29)(30)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54).…”

Section: General Description Of Crayfish Immunitymentioning

confidence: 99%

“…Changes in hemocytes' shape and/or features, in addition to surveying the total hemocyte number (THC), and differential hemocyte count (DHC), as well as the assessment of defensive activities like phagocytosis, cell viability, cytotoxicity, and different physiological changes, have been considered useful assays to investigate the crayfish immune response modulation (4,26,(43)(44)(45). Hemocytosis requires extensive recruitment and infiltration of hemocytes into tissues where infections/stress have been detected (25,30,32,55,56).…”

Section: Cell-mediated Immune Responsementioning

confidence: 99%

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

Bouallegui

2021

Front. Immunol.

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Freshwater crayfish immunity has received great attention due to the need for urgent conservation. This concern has increased the understanding of the cellular and humoral defense systems, although the regulatory mechanisms involved in these processes need updating. There are, however, aspects of the immune response that require clarification and integration. The particular issues addressed in this review include an overall description of the oomycete Aphanomyces astaci, the causative agent of the pandemic plague disease, which affects freshwater crayfish, and an overview of crustaceans’ immunity with a focus on freshwater crayfish. It includes a classification system of hemocyte sub-types, the molecular factors involved in hematopoiesis and the differential role of the hemocyte subpopulations in cell-mediated responses, including hemocyte infiltration, inflammation, encapsulation and the link with the extracellular trap cell death pathway (ETosis). In addition, other topics discussed include the identity and functions of hyaline cells, the generation of neoplasia, and the emerging topic of the role of sessile hemocytes in peripheral immunity. Finally, attention is paid to the molecular execution of the immune response, from recognition by the pattern recognition receptors (PRRs), the role of the signaling network in propagating and maintaining the immune signals, to the effector elements such as the putative function of the Down syndrome adhesion molecules (Dscam) in innate immune memory.

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Effects of X-ray irradiation on haemocytes of Procambarus clarkii (Arthropoda: Decapoda) males

Cited by 6 publications

References 22 publications

Detection and Control of Invasive Freshwater Crayfish: From Traditional to Innovative Methods

Detection and Control of Invasive Freshwater Crayfish: From Traditional to Innovative Methods

Medium‐term feasibility of the management of the invasive crayfish Procambarus clarkii with the sterile males release technique

A Comprehensive Review on Crustaceans’ Immune System With a Focus on Freshwater Crayfish in Relation to Crayfish Plague Disease

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