Effects of X- and UV-irradiation on proteins

Durchschlag, Helmut; Fochler, C.; Feser, B.; Hausmann, S.; Seroneit, T.; Swientek, M.; Swoboda, E.; Winklmair, A.; Wlček, C.; Zipper, Peter

doi:10.1016/0969-806x(95)00138-n

Cited by 26 publications

(22 citation statements)

References 3 publications

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“…Gamma-irradiation of the protein solution caused the disruption of the ordered structure of protein macromolecules as well as degradation, cross-linking and aggregation of polypeptide chains [76]. Similar results were also obtained by UV or X-rays irradiation [77]. However these reactions are difficult to control and not industrially employed for improving protein film formation.…”

Section: Protein Modification To Control and Optimize Film-formationsupporting

confidence: 68%

State of the Art in the Development and Properties of Protein-Based Films and Coatings and Their Applicability to Cellulose Based Products: An Extensive Review

et al. 2015

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Abstract:There is increasing research towards the substitution of petrochemicals by sustainable components. Biopolymers such as proteins, polysaccharides, and lipids derive from a variety of crop sources and most promisingly from waste streams generated during their processing by the agro food industry. Among those, proteins of different types such as whey, casein, gelatin, wheat gluten, soy protein or zein present a potential beyond the food and feed industry for the application in packaging. The general protein hydrophilicity promotes a good compatibility to polar surfaces, such as paper, and a good barrier to apolar gases, such as oxygen and carbon dioxide. The present review deals with the development of protein-based coatings and films. It includes relevant discussion for application in paper or board products, as well as an outlook on its future industrial potential. Proteins with suitable functionalities as food packaging materials are described as well as the different technologies for processing the coatings and the current state of the art about the coating formulations for selectively modulating barrier, mechanical, surface and end of life properties. Some insights onto regulations about packaging use, end of life and perspectives of such natural coating for decreasing the environmental impact of packages are given.

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Section: Protein Modification To Control and Optimize Film-formationsupporting

confidence: 68%

State of the Art in the Development and Properties of Protein-Based Films and Coatings and Their Applicability to Cellulose Based Products: An Extensive Review

et al. 2015

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“…Since this hypothesis was not confirmed by the following characterization steps, the observed size decrease cannot be explained by protein degradation. Another reason for the decreasing particle size due to UV treatment might be a radiation-induced compaction of the proteins, as previously described by Durchschlag [ 39 , 40 , 41 ]. For the particle size distribution of all three samples a PDI in the range of 0.23 to 0.33 was determined.…”

Section: Resultsmentioning

confidence: 99%

Influence of Sterilization and Preservation Procedures on the Integrity of Serum Protein-Coated Magnetic Nanoparticles

et al. 2017

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Protein-coated magnetic nanoparticles are promising candidates for various medical applications. Prior to their application into a biological system, one has to guarantee that the particle dispersions are free from pathogens or any other microbiologic contamination. Furthermore, to find entrance into clinical routine, the nanoparticle dispersions have to be storable for several months. In this study, we tested several procedures for sterilization and preservation of nanoparticle containing liquids on their influence on the integrity of the protein coating on the surface of these particles. For this, samples were treated by freezing, autoclaving, lyophilization, and ultraviolet (UV) irradiation, and characterized by means of dynamic light scattering, determination of surface potential, and gel electrophoresis afterwards. We found that the UV sterilization followed by lyophilization under the addition of polyethylene glycol are the most promising procedures for the preparation of sterilized long-term durable protein-coated magnetic nanoparticles. Ongoing work is focused on the optimization of used protocols for UV sterilization and lyophilization for further improvement of the storage time.

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“…This is affected by the UV light initiating a reaction between two thymine molecules to form a thymine dimer which results in cell inactivation by inhibiting bacterial DNA replication [44], [45]. In addition to these changes, proteins are also expected to undergo denaturation which is depicted by their absorption and fluorescence spectra changes as a function of UV light (∼280 nm) absorption by the bacteria cells [46], [47].…”

Section: Introductionmentioning

confidence: 99%

Identification of Live and Dead Bacteria: A Raman Spectroscopic Study

Dhankhar

Chen

et al. 2019

IEEE Access

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Raman spectroscopy has been used to identify bacterial strains, bacterial components, such as protein and DNA bases, and the ratio of live to dead bacteria before and after exposure to ultraviolet (UV) radiation. New vibrational bands and changes in their intensity as a function of UV irradiation time have been recorded by high resolution Raman spectroscopy which made it possible to determine the mechanism of the UV inactivation of Escherichia coli (E. coli), Serratia marcescens (S. marcescens), and Micrococcus luteus (M. luteus) bacteria in saline solutions. We have also employed a novel, new, handheld spectrometer capable of recording, in situ, within minutes, the absorption, fluorescence, synchronous fluorescence, and Raman spectra of bacteria and other biological species and large molecules. INDEX TERMS Bacteria inactivation, optical spectroscopy, principal component analysis, Raman scattering.

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Effects of X- and UV-irradiation on proteins

Cited by 26 publications

References 3 publications

State of the Art in the Development and Properties of Protein-Based Films and Coatings and Their Applicability to Cellulose Based Products: An Extensive Review

State of the Art in the Development and Properties of Protein-Based Films and Coatings and Their Applicability to Cellulose Based Products: An Extensive Review

Influence of Sterilization and Preservation Procedures on the Integrity of Serum Protein-Coated Magnetic Nanoparticles

Identification of Live and Dead Bacteria: A Raman Spectroscopic Study

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