Effects of whey protein and conjugated linoleic acid on acrolein-induced cardiac oxidative stress, mitochondrial dysfunction and dyslipidemia in rats

Aydın, Birsen; Şekeroğlu, Zülal Atlı; Şekeroğlu, Vedat

doi:10.1016/j.biopha.2018.08.081

Cited by 19 publications

(22 citation statements)

References 55 publications

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“…CLA was administered to the CLA group by gavage at the dose of 200 mg kg −1 once a day regularly for 6 weeks. The dose of CLA used in the experiment, which was equivalent to about 2 g per day in humans, was based on previous research …”

Section: Methodsmentioning

confidence: 99%

Conjugated Linoleic Acid Ameliorates High Fructose‐Induced Hyperuricemia and Renal Inflammation in Rats via NLRP3 Inflammasome and TLR4 Signaling Pathway

Tan

Wan

Chen

et al. 2019

Molecular Nutrition Food Res

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Scope: Conjugated linoleic acid (CLA), a bioactive substance predominantly found in ruminant products, improves insulin resistance and exhibits anti-inflammatory activity. The chief objective of the study is to investigate the effects and potential mechanisms of CLA on high fructose-induced hyperuricemia and renal inflammation. Methods and results: Hyperuricemia and renal inflammation are induced in rats by 10% fructose. Hyperuricemia, insulin resistance, and renal inflammation are evaluated. CLA potently ameliorates fructose-induced hyperuricemia with insulin resistance and significantly reduces the levels of inflammation factors in serum and kidney. It reverses fructose-induced upregulation of glucose transporter 9 (GLUT9) and urate transporter 1 (URAT1) in the kidney. Moreover, CLA dramatically inhibits the activation of the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome. Additionally, CLA suppresses toll-like receptor 4 (TLR4)/myeloid differentiation factor 88 (MyD88) signaling activation to inhibit nuclear factor-kB (NF-kB) signaling in the kidney of fructose-fed rats. Conclusion: CLA ameliorates hyperuricemia along with insulin resistance and renal inflammatory, which may be associated with the suppression of renal GLUT9 and URAT1 in fructose-fed rats. Its molecular mechanism may be related to the inhibition of NLRP3 inflammasome and TLR4/MyD88 signaling pathway. Therefore, CLA may be a promising candidate for preventing fructose-induced hyperuricemia and renal inflammation.

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Section: Methodsmentioning

confidence: 99%

Conjugated Linoleic Acid Ameliorates High Fructose‐Induced Hyperuricemia and Renal Inflammation in Rats via NLRP3 Inflammasome and TLR4 Signaling Pathway

Tan

Wan

Chen

et al. 2019

Molecular Nutrition Food Res

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“…Previous studies on the relation of oxidative stress damage and whey protein supplementation have different conclusions. Some studies believe that supplement whey protein can increase the levels of oxidative stress products [21,22], while others showed whey protein can reduce oxidative damage [23][24][25]. During our experiment session, oxidative stress products MDA was increased by 20.7% on days 14, anti-oxidative enzymes SOD was increased by 13.4% on days 14, and GSH was increased by 16.7% and 29.1% on days 7 and 14 after whey preload.…”

Section: Effects Of Whey Preload On Plasma Stress Hormones In Gdm Womenmentioning

confidence: 65%

Whey protein preloading can alleviate stress adaptation disorder and improve hyperglycemia in women with gestational diabetes mellitus

Feng

Song

et al. 2020

Preprint

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Background Whey protein (WP) intake has been shown to improve hperglycemia. Objective The aim of this study was to investigate the change of stress hormones, oxidative stress and insulin resistance (IR) in women with gestational diabetes mellitus (GDM) after supplement whey protein, in an attempt to gain insights into the prevention and treatment of GDM. Methods 60 GDM women were recruited in this study, and 30 women receive a preload drink containing 25 g whey protein as group GDM-W, and the other 30 women receive control flavoring drink as group GDM, and the trial last for 14 days. Plasma epinephrine (E), noradrenaline (NE), and cortisol were detected, we also investigate levels of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione (GSH). Homeostasismodel assessment of insulin resistance (HOMA-IR) was used to assesse IR. Results In GDM-W group, postprandial blood glucose was decreased significantly on 3d, 5d, 7d and 14d (p < 0.05), plasma 2 h insulin was increased by 7.2%, 8.6% and 20.5% on days 5, 7 and 14 (p < 0.05). HOMA-IR was decreased significantly on days 14 (P < 0.01). MDA was increased by 20.7% on days 14 (P < 0.01), and anti-oxidative enzymes SOD was increased by 13.4% on days 14 (P < 0.01) and GSH was increased by 16.7% and 29.1% on days 7 and 14 (both P < 0.05). Stress hormones E and cortisol were decreased by 10.8% and 19.8% respectively on days 14 (p < 0.05). Conclusions Whey protein supplementation may improve hyperglycemia by alleviate stress disorder and oxidative stress injury in GDM women.

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“…Oral supplementation with Protectamin significantly increased plasma GSH levels in patients with advanced HIV-infection compared to baseline. [12]. Addition of whey protein at a dosage of 0.2 g/kg body weight/d did not halt the loss of GSH.…”

Section: Wpi Immunocalmentioning

confidence: 88%

“…There is also some evidence from intervention trials that whey proteins may decrease plasma GSH levels, cause heart damage and liver injury [12,13]. Oral Table 3.…”

Section: Whey Proteins and Their Ability To Modify Redox Pathwaysstudmentioning

confidence: 99%

“…In addition, this co-treatment with whey protein exacerbated an observed increase in plasma homocysteine levels and creatine kinase levels induced by acrolein, such that the levels of these markers in the co-treatment group were significantly higher than in the controls. This led the authors to hypothesise that intake of acrolein together with whey proteins may cause heart damage in rats [12]. Liver may also be adversely affected by whey treatment.…”

Section: Wpi Immunocalmentioning

confidence: 99%

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Whey proteins: targets of oxidation, or mediators of redox protection

Giblin

Yalçın

Biçim

et al. 2019

Free Radical Research

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Bovine whey proteins are highly valued dairy ingredients. This is primarily due to their amino acid content, digestibility, bioactivities and their processing characteristics. One of the reported bioactivities of whey proteins is antioxidant activity. Numerous dietary intervention trials with humans and animals indicate that consumption of whey products can modulate redox biomarkers to reduce oxidative stress. This bioactivity has in part been assigned to whey peptides using a range of biochemical or cellular assays in vitro. Superimposing whey peptide sequences from gastrointestinal samples, with whey peptides proven to be antioxidant in vitro, allows us to propose peptides from whey likely to exhibit antioxidant activity in the diet. However, whey proteins themselves are targets of oxidation during processing particularly when exposed to high thermal loads and/or extensive processing (e.g. infant formula manufacture). Oxidative damage of whey proteins can be selective with regard to the residues that are modified and are associated with the degree of protein unfolding, with a-Lactalbumin more susceptible than b-Lactoglobulin. Such oxidative damage may have adverse effects on human health. This review summarises how whey proteins can modulate cellular redox pathways and conversely how whey proteins can be oxidised during processing. Given the extensive processing steps that whey proteins are often subjected to, we conclude that oxidation during processing is likely to compromise the positive health attributes associated with whey proteins.

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Effects of whey protein and conjugated linoleic acid on acrolein-induced cardiac oxidative stress, mitochondrial dysfunction and dyslipidemia in rats

Cited by 19 publications

References 55 publications

Conjugated Linoleic Acid Ameliorates High Fructose‐Induced Hyperuricemia and Renal Inflammation in Rats via NLRP3 Inflammasome and TLR4 Signaling Pathway

Conjugated Linoleic Acid Ameliorates High Fructose‐Induced Hyperuricemia and Renal Inflammation in Rats via NLRP3 Inflammasome and TLR4 Signaling Pathway

Whey protein preloading can alleviate stress adaptation disorder and improve hyperglycemia in women with gestational diabetes mellitus

Whey proteins: targets of oxidation, or mediators of redox protection

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