Effects of waterborne Cu exposure in gilthead sea bream (Sparus aurata): A proteomic approach

Isani, Gloria; Andreani, Giulia; Carpenè, Emilio; Molfetta, Sergio Di; Eletto, Daniela; Spisni, Enzo

doi:10.1016/j.fsi.2011.09.005

Cited by 28 publications

(25 citation statements)

References 45 publications

(45 reference statements)

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“…In contrast, both the stability of lysosomes 1 and 2 and MMM showed consistent responses, with the former declining following metal exposure [100, 147], and the latter increasing following exposure to contaminated sediment in the field and Cd exposure in laboratory toxicity testing [117, 148]. Only one study each measured changes in TV, TCF-b1, Pro-S, MAM, MA and CV [72, 103, 145, 147, 149]. Due to the limited number of studies using these immunological parameters these biomarkers were not recommended for Gladstone Harbour.…”

Section: Resultsmentioning

confidence: 99%

“…New techniques such as microarray, proteomics and RNA sequencing (RNASeq) take fish health assessments beyond individual biomarkers to a global analysis of how all genes or proteins within an individual fish respond to contaminants [3, 149, 156]. Approaches such as microarray and proteomics not only extend the number of biomarkers analysed but also provide knowledge of exposure and pathways of injury, and potentially facilitate the identification of new more suitable individual biomarkers for routine monitoring programs [3, 156].…”

Section: Resultsmentioning

confidence: 99%

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A protocol for identifying suitable biomarkers to assess fish health: A systematic review

2017

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BackgroundBiomarkers have been used extensively to provide the connection between external levels of contaminant exposure, internal levels of tissue contamination, and early adverse effects in organisms.ObjectivesTo present a three-step protocol for identifying suitable biomarkers to assess fish health in coastal and marine ecosystems, using Gladstone Harbour (Australia) as a case study.MethodsPrior to applying our protocol, clear working definitions for biomarkers were developed to ensure consistency with the global literature on fish health assessment. First, contaminants of concern were identified based on the presence of point and diffuse sources of pollution and available monitoring data for the ecosystem of interest. Second, suitable fish species were identified using fisheries dependent and independent data, and prioritised based on potential pathways of exposure to the contaminants of concern. Finally, a systematic and critical literature review was conducted on the use of biomarkers to assess the health of fish exposed to the contaminants of concern.Results/DiscussionWe present clear working definitions for bioaccumulation markers, biomarkers of exposure, biomarkers of effect and biomarkers of susceptibility. Based on emission and concentration information, seven metals were identified as contaminants of concern for Gladstone Harbour. Twenty out of 232 fish species were abundant enough to be potentially suitable for biomarker studies; five of these were prioritised based on potential pathways of exposure and susceptibility to metals. The literature search on biomarkers yielded 5,035 articles, of which 151met the inclusion criteria. Based on our review, the most suitable biomarkers include bioaccumulation markers, biomarkers of exposure (CYP1A, EROD, SOD, LPOX, HSP, MT, DNA strand breaks, micronuclei, apoptosis), and biomarkers of effect (histopathology, TAG:ST).ConclusionOur protocol outlines a clear pathway to identify suitable biomarkers to assess fish health in coastal and marine ecosystems, which can be applied to biomarker studies in aquatic ecosystems around the world.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A protocol for identifying suitable biomarkers to assess fish health: A systematic review

2017

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“…The studies identified include one specifically associated with the selection of resistant gut bacteria in pigs (Amachawadi et al, 2011;Bundy et al, 2008;Chen D and Chan K, 2009;Isani et al, 2011;Santos et al, 2010;Song et al, 2009). The studies used six animal systems (three for fish) and two (hepatocyte) cell systems.…”

Section: Hazardmentioning

confidence: 99%

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

Pielaat

Barker

Hendriksen

et al. 2013

EFSA Supporting Publications

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Suggested citation: Pielaat A, Barker GC, Hendriksen P, Peijnenburg A and Kuile BH, 2013. A foresight study on emerging technologies: State of the art of omics technologies and potential applications in food and feed safety. REPORT 1: Review on the state of art of omics technologies in risk assessment related to food and feed safety. EFSA supporting publication 2013:EN-495, 126 pp. ABSTRACTThis report consists of a review of the available scientific evidence on the state of art of omics technologies with a particular focus on applications in the area of food and feed safety. The report gives a description of the different types of omics technologies with respect to recent progresses made on the application of these techniques in chemical and microbiological risk assessment. Hereto, case studies were selected in order to cover different areas under the remit of EFSA. In this review each case study has been investigated with respect to its hazard, experimental methodologies, (statistical) data analysis and its link to food/feed safety risk assessment. Finally, the added value of omics technologies as compared to classical risk assessment and current limitations of omics technologies for their application in chemical and microbiological food safety risk assessment has been discussed. This investigation shows that most of the studies on chemical agents focus on mode of action identification and a search for biomarkers using transcriptomics. As for microbial food safety, omics have not yet been successfully applied often. When applied, they never target the host, but focus on aspects of the pathogen, e.g. in detection or source attribution.

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“…Besides these, immunoproteomic studies also encompass results on immunostimulants [35], antimicrobial peptides [143][144][145] and composition of mucosal secretions [146,147], along with other topics related to a better understanding of mechanistic aspects of immune responses to certain stimuli [40,[148][149][150]. The study of the impact of xenobiotics in aquaculture has also benefited from proteomics technologies, with several studies done on the impact of biotoxins [141,[151][152][153], pharmaceuticals [58,154,155], hormones [15,46,156], metals [157,158] and other pollutants [43,45,67,[159][160][161][162][163] on the proteomes of aquaculture aquatic organisms. Finally, there are also some studies correlating environmental sources of stress in aquaculture (hypoxia [57], anoxia [26,[164][165][166], hyperoxygenation [167], osmotic [168][169][170][171][172][173] and temperature [174]…”

mentioning

confidence: 99%

“…In the case of salinity changes, proteomic studies in fish often target osmoregulatory organs (like gills and kidney) and generally show similar signs of cellular stress, particularly affecting cytoskeletal dynamics, betaine metabolism and aldehyde detoxification [169,[171][172][173]. Regarding the effect of metals, biotoxins and other environmental toxicants on the proteome of fish, most studies focus on the liver, generally displaying increased signs of oxidative stress, changes in immune/ inflammatory effectors, as well as induction of transporters and detoxification enzymes [43,154,157,158,[160][161][162][163][185][186][187]. But, again, there is a general overlap in terms of affected pathways, when comparing to hypoxia-type stressors.…”

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confidence: 99%

PROTEOMICS in aquaculture: Applications and trends

Rodrigues

Silva

Dias

et al. 2012

Journal of Proteomics

122

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Effects of waterborne Cu exposure in gilthead sea bream (Sparus aurata): A proteomic approach

Cited by 28 publications

References 45 publications

A protocol for identifying suitable biomarkers to assess fish health: A systematic review

A protocol for identifying suitable biomarkers to assess fish health: A systematic review

A foresight study on emerging technologies: State of the art of Omics technologies and potential applications in food and feed safety

PROTEOMICS in aquaculture: Applications and trends

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