Effects of water-soluble antioxidant from spinach, NAO, on doxorubicin-induced heart injury

Breitbart, Eyal; Lomnitski, Liat; Nyska, Abraham; Malik, Zafar Ahmad; Bergman, Margalit; Sofer, Yossi; Haseman, Joseph K.; Grossman, Shlomo

doi:10.1191/096032701680350604

Cited by 30 publications

(15 citation statements)

References 37 publications

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“…Similarly to many other studies, the DXR-induced myocardial damage reported here was associated with clear myocardial histopathological lesions (myocyte necrosis, degeneration, and inflammatory cell infiltrations). 3,4,5,25 Similar to these studies, significant cytoplasmic vacuolization, myofibrillar disorganization and myofibrillar losses were observed in the histopathological examination of the cardiac tissues in the DXR administered rats compared to the control group, in our study.…”

Section: -21supporting

confidence: 88%

Cardioprotective Effect of Clarithromycin on Doxorubicin-Induced Cardiac Toxicity in Rats.

Doğan¹,

Fırıncı²,

Balci³

et al. 2014

UHOD

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Doxorubicin is an anthracycline group antibiotic and has long been used as an antineoplastic agent. The most important side effect that limits the usage of doxorubicin is cardiotoxicity, which is observed at cumulative doses. We investigated the protective effect of clarithromycin that is known to have antioxidant and anti-inflammatory effects against Doxorubicin related cardiotoxicity. The aim of our study was to evaluate the effects of clarithromycin in the antioxidant enzyme status and myocardium of doxorobucine-treated rats. A total of 28 adult, male Wistar rats (200-250 g) were divided into 4 groups. Group I was the control group, rats in group 2 were administered doxorubicin, rats in group 3 were administered clarithromycin, and rats in group 4 were administered Doxorubicin + Clarithromycin. Following the scarification of all rats, antioxidant (glutathione) and oxidant (malodialdehyde) levels were measured in the cardiac tissue. Additionally, the cardiac muscles were evaluated histopathologically via hematoxylin eosin staining. The antioxidant amount (glutathione) was significantly higher in the treatment group compared to the doxorubicin group (p= 0.025), whereas the amount of oxidant (malondialdehyde) was significantly lower (p= 0.022). The histopathological examination revealed significant cardiotoxicity in the Doxorubicin group and significant reduction in the cardiotoxicity in the Doxorubicin + Clarithromycin group. The results obtained in this study provide evidence for the usefulness of the clarithromycin as a cardioprotective agent. Grup 1 kontrol grubuydu, grup 2' de ki ratlara doksorubisin, grup 3' de ki ratlara klaritromisin ve grup 4' e doksorubisin+klaritromisin (tedavi grubu) uygulandı. Skarifikasyonu takiben tüm ratların kalp dokusunda antioksidan (glutatyon) ve oksidan (malodialdehid) düzeyleri ölçüldü.Buna ek olarak myokard dokusu hematoksilen-eozin ile boyanarak histopatolojik olarak değerlendirildi. Antioksidan (glutatyon) miktarı, tedavi grubunda doksorubisin grubuyla karşılaştırıldığında önemli derecede yüksekti (p= 0.025), oysa oksidan (malodialdehid) miktarı önemli derecede düşük saptandı (p= 0.022). Histopatolojik değerlendirmede doksorubisin grubunda ciddi kardiyotoksisite saptanırken doksorubisin + klaritromisin uygulanan grupta kardiyotoksisite de ciddi azalma belirlendi. Bu çalışmada elde edilen sonuç, klaritromisinin doxorubisine bağlı kardiyotoksitenin engellenmesinde kullanılabilecek kardiyoproduktif etkiye sahip bir ajan olduğudur.

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Section: -21supporting

confidence: 88%

Cardioprotective Effect of Clarithromycin on Doxorubicin-Induced Cardiac Toxicity in Rats.

Doğan¹,

Fırıncı²,

Balci³

et al. 2014

UHOD

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“…Its efficiency was tested and proven in a variety of biological systems in vivo and in vitro (Breitbart et al, 2001;Lomnitski et al, 2000aLomnitski et al, , 2000bLomnitski et al, , 2003Nyska et al, 2001Nyska et al, , 2003.…”

Section: Introductionmentioning

confidence: 99%

Synergistic activity between a spinach-derived natural antioxidant (NAO) and commercial antioxidants in a variety of oxidation systems

Hait-Darshan¹,

Grossman²,

Bergman³

et al. 2009

Food Research International

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“…The high level of doxorubicin could damage membranes, proteins (e.g., enzymes, structural, and receptors), and DNA that may lead to cardiac dysfunction and apoptosis (Arola et al 2000;Yamaoka et al 2000;Green & Leeuwenburgh 2002;Kumar et al 2002). While the cytoprotective effect of the commonly used antioxidant vitamins (ascorbic acid and vitamin E) remains controversial (Legha et al 1982;Shimpo et al 1991b;Quiles et al 2002) several naturally occurring antioxidants have demonstrated protective role against doxorubicin cardiotoxicity (Chopra et al 1995;Dorr 1996;Kozluca et al 1996;DeAtley et al 1999;Breitbart et al 2001;Abd-Allah et al 2002;Bagchi et al 2003). Therefore, the purpose of this study is to establish a high throughput screening of natural antioxidants for their cardioprotective effect against doxorubicin cardiotoxicity.…”

mentioning

confidence: 99%

Screening of Antioxidants from Medicinal Plants for Cardioprotective Effect against Doxorubicin Toxicity

Wattanapitayakul

Chularojmontri

Herunsalee

et al. 2005

Basic Clin Pharma Tox

103

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Doxorubicin is an important and effective anticancer drug widely used for the treatment of various types of cancer but its clinical use is limited by dose-dependent cardiotoxicity. Elevated tissue levels of cellular superoxide anion/ oxidative stress are a mechanism by which doxorubicin-induced cardiotoxicity. Selected medicinal plant extracts were tested for their antioxidant capacity and cardioprotective effect against doxorubicin-induced cardiotoxicity. The cardiac myoblasts H9c2 were incubated with the antioxidants ascorbic acid, trolox, N-acetylcysteine or selected medicinal plant extracts including; 1) ethanolic extracts from Curcuma longa L-EtOH Phyllanthus emblica L-EtOH, and Piper rostratum Roxb-EtOH; and 2) water extracts from Curcuma longa L-H 2 O and Morus alba L-H 2 O. The cardioprotective effects of these extracts were evaluated by crystal violet cytotoxicity assay. IC50s of doxorubicin were compared in the presence or absence of ascorbic acids, trolox, N-acetylcysteine or plant extracts. Morus alba L-H 2 O showed the highest antioxidant properties evaluated by ferric reducing/antioxidant power assay. Ascorbic acid and N-acetylcysteine had modest effects on the protection of doxorubicin-induced cytotoxicity while trolox showed insignificant protective effect. All plant extracts protected cardiac toxicity at different degrees except that Curcuma longa L-EtOH had no protective effect. Phyllanthus emblica-EtOH (100 mg/ml) showed the highest cardioprotective effect (∂12-fold doxorubicin IC50 increase). The data demonstrate that antioxidants from natural sources may be useful in the protection of cardiotoxicity in patients who receive doxorubicin.Doxorubicin is an effective and broadspectrum antineoplastic agent used in the treatment of a variety of haematologic and solid malignacies, such as leukaemias, bladder, lung, and breast cancers, Hodgkin's and non-Hodgkins lymphomas, and ovarian cancer. Its clinical uses are often limited by the adverse effect cardiotoxicity. An initial acute effect includes hypotension and transient electrocardiographic abnormalities reported up to 41% of patients. The chronic effects may occur several weeks or months after cumulative doxorubicin administration, and the occurrence is dose-dependent cardiomyopathy which accounts for as high as 50% mortality within 2 years after diagnosis (Felker et al. 2000;Jensen et al. 2002). Recent studies demonstrate that increased reactive oxygen species generation, especially superoxide and hydrogen peroxide, is implicated in the mechanisms of doxorubicin-induced cardiotoxicity (Kalivendi et al. 2001;Wang et al. 2004). The high level of doxorubicin could damage membranes, proteins (e.g., enzymes, structural, and receptors), and DNA that may lead to cardiac dysfunction and apoptosis (Arola et al. 2000;Yamaoka et al. 2000;Green & Leeuwenburgh 2002;Kumar et al. 2002). While the cytoprotective effect of the commonly used antioxidant vitamins (ascorbic acid and vitamin E) remains controversial (Legha et al. 1982;Shimpo et al. 1991b;Q...

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Effects of water-soluble antioxidant from spinach, NAO, on doxorubicin-induced heart injury

Cited by 30 publications

References 37 publications

Cardioprotective Effect of Clarithromycin on Doxorubicin-Induced Cardiac Toxicity in Rats.

Cardioprotective Effect of Clarithromycin on Doxorubicin-Induced Cardiac Toxicity in Rats.

Synergistic activity between a spinach-derived natural antioxidant (NAO) and commercial antioxidants in a variety of oxidation systems

Screening of Antioxidants from Medicinal Plants for Cardioprotective Effect against Doxorubicin Toxicity

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