Effects of Water on the Stability of Phospholipid Bilayers: The Problem of Imbibition Damage in Dry Organisms

Crowe, John H.; Crowe, Lois M.; Hoekstra, Folkert A.; Wiström, Christina Aurell

doi:10.2135/cssaspecpub14.c1

Cited by 8 publications

(6 citation statements)

References 33 publications

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“…Membrane phase behavior is commonly monitored by observing the evolution of the position of the symmetric CH 2 stretching band at approximately 2850 cm −1 (vCH 2 symmetric) with cooling and subsequent heating ( Fig. 3) (Crowe et al 1989). Figure 3 shows the membrane phase behavior for two population of Lb.…”

Section: Membrane Lipid Phase Transition (Lα ⇔ Lβ)mentioning

confidence: 99%

“…As a result, during freezing or drying, the various phospholipids enter their respective gel phases at different temperatures, and the gel and liquidcrystal phases transiently coexist. The gel-phase domains would exclude more fluid domains, and in such two-phase systems, membranes are expected to leak during thawing or rehydration with potentially negative consequences for cell survival (Crowe et al 1989). Consequently, in addition to the determination of the lipid phase transition temperatures (Ts (following cooling) and Tm (following heating)), other useful parameters can be obtained from the membrane lipid transition curves: (i) the broadness of the transition indicates lipid heterogeneity (Oldenhof et al 2005;Gautier et al 2013) and possible phase separation due to the coexistence of rigid and fluid domains (Hazel and Williams 1990;Hazel 1995); (ii) the wavenumber increase at high and/or low temperatures denotes high disorder and fluidity (Gautier et al 2013); and (iii) hysteresis between cooling and heating has been ascribed to irreversible phenomena occurring during freezing, probably lateral phase separation (Gautier et al 2013).…”

Section: Membrane Lipid Phase Transition (Lα ⇔ Lβ)mentioning

confidence: 99%

“…During drying processes, removal of unfrozen water results in profound changes in the physical properties of biomolecules, particularly phospholipids and proteins (Crowe et al 1989). A decrease of the lateral spacing of the polar headgroups and the subsequent packing of the hydrocarbon chains lead to a considerable increase of the membrane lipid phase transition after drying (Potts 1994).…”

Section: Modification Of Membrane Properties Following Stabilization mentioning

confidence: 99%

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Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fonseca

Pénicaud

Tymczyszyn

et al. 2019

Appl Microbiol Biotechnol

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Production of lactic acid bacteria starters for manufacturing food, probiotic, and chemical products requires the application of successive steps: fermentation, concentration, stabilization, and storage. Despite process optimization, losses of bacterial viability and functional activities are observed after stabilization and storage steps due to cell exposure to environmental stresses (thermal, osmotic, mechanical, and oxidative). Bacterial membrane is the primary target for injury and its damage is highly dependent on its physical properties and lipid organization. Membrane fluidity is a key property for maintaining cell functionality, and depends on lipid composition and cell environment. Extensive evidence has been reported on changes in membrane fatty acyl chains when modifying fermentation conditions. However, a deep characterization of membrane physical properties and their evolution following production processes is scarcely reported. Therefore, the aims of this mini-review are (i) to define the membrane fluidity and the methods used to assess it and (ii) to summarize the effect of environmental conditions on membrane fluidity and the resulting impact on the resistance of lactic acid bacteria to the stabilization processes. This will make it possible to highlight existing gaps of knowledge and opens up novel approaches for future investigations.

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Section: Membrane Lipid Phase Transition (Lα ⇔ Lβ)mentioning

confidence: 99%

Section: Membrane Lipid Phase Transition (Lα ⇔ Lβ)mentioning

confidence: 99%

Section: Modification Of Membrane Properties Following Stabilization mentioning

confidence: 99%

See 1 more Smart Citation

Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Fonseca

Pénicaud

Tymczyszyn

et al. 2019

Appl Microbiol Biotechnol

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“…Membranes are physiologically active in the liquid, crystalline state and in desiccated tissue may exist in the gel state. In several studies, Crowe et al (1989bCrowe et al ( , 1989cCrowe et al ( , 1996aCrowe et al ( , 1997c found that the transition temperature for phospholipids varied with water content and the presence of sugars associated with lipid head groups; particularly, sucrose depressed the Tm in dry liposomes.…”

Section: Physiological and Chemical Changes During Acquisition Of Desmentioning

confidence: 99%

“…Several investigators have reported ultrastructural, physiological, chemical, and biochemical changes during the transition from desiccation intolerance to tolerance. Among these are decreases in the degree of vacuolation (Berjak et al, 1992;Farrant et al, 1997), alignment of lipid bodies along the plasma membrane Perdomo and Bums, 1998), accumulation or changes in the ratios of specific sugars, (Crowe et al, 1988;Kuo et al, 1988;Chen and Bums, 1990;Black et al, 1996), changes in phospholipids and lipid composition (Crowe, 1989b(Crowe, , 1989cChen and Bums, 1991;Tetteroo et al, 1996), and expression of late embryogenesis abundant (LEA) proteins (Dure, 1981;Farrant et al, 1993;Han et al, 1997). However, the specific functional mechanism(s) by which these changes contribute to desiccation tolerance is not completely understood.…”

Section: Introductionmentioning

confidence: 99%

Changes in embryo drying rates, the alignment of lipid bodies along the plasma membrane, and dehydrin-like proteins during the acquisition of desiccation tolerance in maize seed

Córdova-Téllez

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Preserving maize (Zea mays L.) seed quality during mechanical drying is an important concern in the seed industry. In this study, preconditioning (slow drying prior to fast drying treatments) was used to investigate embryo-drying rates, the alignment of lipid bodies along the plasma membrane, and dehydrin-like protein profiles during the acquisition of desiccation tolerance in maize seed. Ears of hybrid maize [B73 x (H99 x H95)] were harvested in 1998, 1999, and 2000 at ca. 55,50,40 and 32% moisture content (mc) and subjected to preconditioning (PC) (ear drying at 35°C and 0.47 m/s airflow rate) for 0,12,24, 36, and 48h prior to fast drying (shelled seed, 35°C and 5.10 m/s airflow rate) treatments to decrease moisture to ca. 13%. The mc of intact seed harvested at >50% mc decreased ca. 20% points (pts) during the PC phase (0 to 48h) whereas the embryo mc decreased about 3% pts. With advances in maturity, embryo-drying rates increased while intact seed drying rates decreased. Under rapid drying conditions, on the other hand, embryo mc declined at very rapid rates down to ca. 40%. These rapid drying rates slightly decreased with increases in maturation, and, consequently, embryo dehydration seemed to occur in a more organized fashion. Similar effects, but to a lesser degree, were observed with increases in PC time prior to fast drying. Alignment of lipid bodies occurred first in the root cap, followed by outer and inner core cells. However, cell plasmolysis and aberrations in or perhaps coalescence of lipid bodies, were evident in seed harvested at mc >40% and subjected to fast drying without PC. These aberrations decreased in severity with PC and field maturation and appeared to be associated with partial or incomplete lipid body alignment prior to fast drying. In seed subjected to fast drying conditions, there is a slight but detectable increase in dehydrin-like proteins with increases in PC time prior to fast drying, particularly in seed harvested at 55 vii and 50% mc. Immunogold-labeling of these proteins indicated that they were located in an interspersed manner on surfaces of the plasma membrane, lipid bodies, and throughout the cytoplasm. Alignment of lipid bodies along the plasma membrane appear to be associated with slight decreases in embryo drying rates and more organized dehydration in seed dried under the fast drying conditions. Slow embryo drying rates during preconditioning may be associated with embryo position on the ear. These slow drying rates or maintenance of high hydration levels for longer period coincided with increases in accumulation of dehydrin-like proteins. Decreases in embryo drying rates and more organized dehydration, better alignment of lipid bodies along the plasma membrane, and higher accumulation of dehydrinlike proteins were associated with lesser cell solute leakage and better performance in germination and vigor tests. We conclude that slower embryo drying rates to threshold levels may be crucial to acquiring the ability to tolerate faster dehydration. Alignment of lipid bodie...

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Desiccation and supra‐zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to cryopreservation

Lee

Comizzoli

2019

Molecular Reproduction Devel

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Understanding cellular and molecular damages in oocytes during exposure to extreme conditions is essential to optimize long‐term fertility preservation approaches. Using the domestic cat (Felis catus) model, we are developing drying techniques for oocytes’ germinal vesicles (GVs) as a more economical alternative to cryopreservation. The objective of the study was to characterize the influence of desiccation on nuclear envelope conformation, chromatin configuration, and the relative fluorescent intensities of histone H3 trimethylation at lysine 4 (H3K4me3) and at lysine 9 (H3K9me3) compared to vitrification. Results showed that higher proportions of dried/rehydrated GVs maintained normal nuclear envelope conformation and chromatin configuration than vitrified/warmed counterparts. Both preservation methods had a similar influence on epigenetic patterns, lowering H3K4me3 intensity to under 40% while maintaining H3K9me3 levels. Further analysis revealed that the decrease of H3K4me3 intensity mainly occurred during microwave dehydration and subsequent rehydration, whereas sample processing (permeabilization and trehalose exposure) or storage did not significantly affect the epigenetic marker. Moreover, rehydration either directly or stepwise with trehalose solutions did not influence the outcome. This is the first report demonstrating that the incidence of GV damages is lower after desiccation/rehydration than vitrification/warming.

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Effects of Water on the Stability of Phospholipid Bilayers: The Problem of Imbibition Damage in Dry Organisms

Cited by 8 publications

References 33 publications

Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Factors influencing the membrane fluidity and the impact on production of lactic acid bacteria starters

Changes in embryo drying rates, the alignment of lipid bodies along the plasma membrane, and dehydrin-like proteins during the acquisition of desiccation tolerance in maize seed

Desiccation and supra‐zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to cryopreservation

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