Effects of water chemistry and surface contact on the toxicity of silver nanoparticles to Bacillus subtilis

Yi, Jun Koo; Cheng, Jack Chin Pang

doi:10.1007/s10646-017-1796-1

Cited by 9 publications

(3 citation statements)

References 32 publications

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“…The nanomaterial coating may also be a confounding factor in toxicity assessments [20,24,30,46], though oleic acid was used in the present study and is regarded as non-toxic [47]. AgNPs coated with fatty acid (oleic acid), used in the present study, are quite stable and do not dissolute as much silver ion as previously reported [22,48]. The limited release of silver ion from the AgNPs used might explain the observed limited toxicity in fish embryos.…”

Section: Discussionmentioning

confidence: 59%

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Qiang

Arabeyyat

et al. 2020

IJMS

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Silver nanoparticles (AgNPs) are widely used in commercial applications as antimicrobial agents, but there have recently been increasing concerns raised about their possible environmental and health impacts. In this study, zebrafish embryos were exposed to two sizes of AgNP, 4 and 10 nm, through a continuous exposure from 4 to 96 h post-fertilisation (hpf), to study their uptake, impact and molecular defense responses. Results showed that zebrafish embryos were significantly impacted by 72 hpf when continuously exposed to 4 nm AgNPs. At concentrations above 0.963 mg/L, significant in vivo uptake and delayed yolk sac absorption was evident; at 1.925 mg/L, significantly reduced body length was recorded compared to control embryos. Additionally, 4 nm AgNP treatment at the same concentration resulted in significantly upregulated hypoxia inducible factor 4 (HIF4) and peroxisomal membrane protein 2 (Pxmp2) mRNA expression in exposed embryos 96 hpf. In contrast, no significant differences in terms of larvae body length, yolk sac absorption or gene expression levels were observed following exposure to 10 nm AgNPs. These results demonstrated that S4 AgNPs are available for uptake, inducing developmental (measured as body length and yolk sac area) and transcriptional (specifically HIF4 and Pxmp2) perturbations in developing embryos. This study suggests the importance of particle size as one possible factor in determining the developmental toxicity of AgNPs in fish embryos.

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Section: Discussionmentioning

confidence: 59%

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Qiang

Arabeyyat

et al. 2020

IJMS

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“…The morphologic and structural properties of bacteria between B. subtilis and SEBS was monitored using a scanning electron microscope (SEM) and transmission electron microscopy (TEM). The bacteria of B. subtilis and SEBS was concentrated via centrifugation at 3, 000 rpm, and immersed in a 5% glutaraldehyde solution for 24 h [24]. Se concentration in the supernatant and precipitate of B. subtilis and SEBS fermented medium was calculated using atomic absorption spectrometry and the live bacteria was enumerated with yeast extract peptone dextrose medium after term serial dilution.…”

Section: Methodsmentioning

confidence: 99%

SEBS colonized in ileal mucosa, optimized bacterial composition, improved Se level and activated TLR2－NF-kB1 signaling pathway to regulate β-defensin 1 expression

Yang¹,

Wang²,

Zhang

et al. 2020

Preprint

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Abstract Background Both selenium (Se) and probiotic bacillus improve immunity. Beta defensin 1 (BD1), a component of intestinal mucosal immunity, could be up-regulated in dietary selenium enriched bacillus subtilis (SEBS) supplementation. SEBS was supplemented in the culture medium of mouse intestinal crypt cells and the diets of chicks to observe the effects of SEBS on BD1 in the intestine by colonization of bacillus subtilis, its recognition and signaling pathway, bacterial composition optimization, and biological functions of Se. Results BD1 was formed in intestinal crypt cells and secreted into the lumen through the villi brush border. BD1 was up-regulated in distal ileum segments with SEBS and bacillus subtilis colonization. This occurred through the recognition of toll like receptor 2 and the NF-kB1 signaling pathway (TLR2-MyD88།NF-kB1), this increased expression was further enhanced with Se combination. Pro-inflammatory cytokines, TNF-α, IL-1β, and IL-6, were up-regulated with bacillus subtilis supplementation, while this up-regulation was inhibited with Se. Colonization of bacillus subtilis in distal segments of the ileum improved bacterial diversity, while reducing the number of endogenous Salmonella and lactobacilli sp. in ileal mucous membranes with SEBS supplementation. Species of unclassified Lachnospiraceae, uncultured Anaerosporobacter, Ruminococcaceae_UCG-014, Peptococcus, and Lactobacillus salivarius, and unclassified Butyricicoccus were substantial in ileal mucous membranes to promote BD1 concentration. Conclusion SECB, colonized in the ileal mucous membrane, optimized bacterial composition, enhanced BD1 secretion through activation of the TLR2-MyD88།NF-kB1 signaling pathway, and reduced pro-inflammatory factors. Our results suggest a new avenue for the combination of probiotic bacteria and essential micro-element selenium to improve intestinal mucosal immunity, increase defense against cold stress, and reduce illness incidence and mortality.

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“…The morphological and structural properties of B. subtilis yb-114,246 and SEBS were monitored using a scanning electron microscope (SEM) and a transmission electron microscope (TEM). B. subtilis yb-114,246 and SEBS were concentrated via centrifugation at 3, 000 rpm, and immersed in a 5% glutaraldehyde solution for 24 h [19]. Se concentration in the supernatant and precipitate of B. subtilis yb-114,246 and SEBS fermented medium was calculated using atomic absorption spectrometry and the live bacteria were enumerated with yeast extract peptone dextrose medium after term serial dilution.…”

Section: Preparation and Analyses Of Bs And Sebsmentioning

confidence: 99%

Selenium Enriched Bacillus Subtilis Yb-1114246 Improved Growth and Immunity of Broiler Chickens Through Modified Ileal Bacterial Composition

Yang

Wang

Huang

et al. 2021

Preprint

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Background: Selenium-enriched Bacillus subtilis (SEBS) was made to add into the diets of broiler chickens to observe combined effects on the bacterial composition and immunity of ileum through its colonization on the site of intestinal mucous membrane to improve the body growth and immunity. Five hundred 1-d-old chickens were divided into five groups randomly: Control, inorganic Se, Bacillus subtilis (B. subtilis), SEBS, and antibiotic. The feed duration was 42 days (d). Growth performance was recorded and calculated. The strain of B. subtilis and SEBS colonizing in the gastrointestinal tract (GIT) were investigated using fluorescence in situ hybridization (FISH) assay and quantitative real-time polymerase chain reaction (qPCR). The V3-V4 hypervariable regions of the bacterial region of 16S rRNA gene were sequenced. Results: After 42 d of treatment, chicks feed SEBS or Bacillus subtilis had higher body weights than the control chicks or those given inorganic Se. SEBS colonized in distal segments of the ileum improved bacterial diversity, while reducing the number of endogenous pathogen and increasing the number of Lactobacillus sp. in ileal mucous membranes. Species of unclassified Lachnospiraceae, uncultured Anaerosporobacter, Peptococcus, Lactobacillus salivarius, and Ruminococcaceae_UCG-014, and unclassified Butyricicoccus in ileal mucous membranes played an important role in promoting immunity, such as tumor necrosis factor-α and interferon-β through spearman’s analysis. Dietary supplementary Se also improved bacterial composition of ileal mucous membranes. SEBS colonization in the ileal mucous membrane optimized bacterial composition, more enhanced the abundances of metabolic and immune genes on body growth and immunity. Conclusions: SEBS improved body growth performance and immunity of broiler chickens through colonization in the ileal mucous membrane and modified ileal bacterial composition.

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Effects of water chemistry and surface contact on the toxicity of silver nanoparticles to Bacillus subtilis

Cited by 9 publications

References 32 publications

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

Silver Nanoparticles in Zebrafish (Danio rerio) Embryos: Uptake, Growth and Molecular Responses

SEBS colonized in ileal mucosa, optimized bacterial composition, improved Se level and activated TLR2－NF-kB1 signaling pathway to regulate β-defensin 1 expression

Selenium Enriched Bacillus Subtilis Yb-1114246 Improved Growth and Immunity of Broiler Chickens Through Modified Ileal Bacterial Composition

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