Effects of water-borne cadmium on the skin of the common carp (Cyprinus carpio)

Iger, Y.; Lock, R.A.C.; Meij, J.C.A. van der; Bonga, S.E. Wendelaar

doi:10.1007/bf00203561

Cited by 63 publications

(27 citation statements)

References 21 publications

(33 reference statements)

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“…There are few scientific studies aimed specifically at the putative effect of cadmium on fish skin cells and most have been done in vivo (Sovenyi and Szakolczai, 1993;Iger et al, 1994). Our group is attempting to establish an in vitro model for the study of the pernicious effect of pollutants on epithelial cells covering the fish body and on the immunomodulating effects of pollutants, so that the interference of contaminants with the development of immunoprotection after vaccination can be assessed.…”

Section: Discussionmentioning

confidence: 99%

“…This study compared the results using two approaches: a clonogenic assay and a growth assay. Epithelial cells were chosen because the epidermis forms the primary barrier between an individual and its environment and is the primary target cell type for pollution-related carcinogenesis (Langdon, 1988;Iger et al, 1994). Although the epithelial barrier is vulnerable to chemical attack, it has received very little attention in toxicological studies.…”

Section: Introductionmentioning

confidence: 99%

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The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals

Lyons-Alcantara¹,

Tarazona²,

Mothersill³

1996

Cell Biol Toxicol

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The differential cytotoxic effects of cadmium on fish and mammalian epithelial cells in established and primary culture were assessed by looking at the reduction of the colony-forming ability and reduction in the extent of growth. The influence of medium composition on the toxicity of cadmium was also studied using serum-free and serum-containing media. The experiments using immortalized cell lines showed that mammalian cells were more sensitive than fish cells to cadmium. Both cell types were grown at the same serum concentration. However, using the normal primary system, human epithelial tissue explants showed less sensitivity to cadmium than did similar cultures from rainbow trout. It is likely that cellular mechanisms of cadmium resistance in the different cell types are responsible for these effects. As expected, cadmium proved to be more toxic when tested in serum-free medium. With fish skin primary cultures, reduction of cell numbers was observed at concentrations as low as 5 mumol/L in serum-free medium compared to 100 mumol/L in serum-containing medium. This was found to be due to the reduction in the activity of free cadmium ions, caused by the presence of serum in the medium. It is concluded that serum-free formulations are probably preferable when testing pollutants in vitro. The results highlight the importance of establishing species-specific pollution tests and standardizing variables. In summary, the results show species and culture media differences in cadmium toxicity and suggest that caution is required when extrapolating results for pollutant effects from one in vitro system to another.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals

Lyons-Alcantara¹,

Tarazona²,

Mothersill³

1996

Cell Biol Toxicol

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“…Cadmium exposure in Mozambique tilapia (Oreochromis mossambicus) prompted higher SOD, CAT, and GPX activities in liver and kidney (Basha and Rani, 2003). Cadmium has been shown to induce apoptosis in the epithelial cells of common carp (Iger et al, 1994) and trout skin (Lyons-Alcantara et al, 1998). Recently, Risso-de Faverney et al (2004) clearly related cell death to the oxidative stress caused by Cd in trout hepatocytes.…”

Section: Antioxidant Defences and Xenobioticsmentioning

confidence: 97%

Antioxidant Defenses in Fish: Biotic and Abiotic Factors

Martínez‐Álvarez

Morales

Sanz

2005

Rev Fish Biol Fisheries

978

456

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“…Changes to mucus producing cells have been associated with both TNBSinduced enterocolitis and skin damage (Iger et al, 1994;Fleming et al, 2010). We analyzed the same series of TNBS doses with whole-mount Alcian blue staining to detect acidic mucus producing cells.…”

Section: Histological Analysis Of Tnbs-induced Enterocolitismentioning

confidence: 99%

A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents

Oehlers

Flores

Okuda

et al. 2010

Developmental Dynamics

120

168

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Inflammatory bowel disease (IBD) results from dysfunctional interactions between the intestinal immune system and microbiota, influenced by host genetic susceptibility. Because a key feature of the pathology is intestinal epithelial damage, potential disease factors have been traditionally analyzed within the background of chemical colitis models in mice. The zebrafish has greatly complemented the mouse for modeling aspects of disease processes, with an advantage for high content drug screens. Larval zebrafish exposed to the haptenizing agent trinitrobenzene sulfonic acid (TNBS) displayed impaired intestinal homeostasis and inflammation reminiscent of human IBD. There was a marked induction of pro-inflammatory cytokines, the degradative enzyme mmp9 and leukocytosis. Enterocolitis was dependent on microbiota and Toll-like receptor signaling, that can be ameliorated by antibiotic and anti-inflammatory drug treatments. This system will be useful to rapidly interrogate in vivo the biological significance of the IBD candidate genes so far identified and to carry out pharmacological modifier screens. Developmental Dynamics 240:288-298,

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Effects of water-borne cadmium on the skin of the common carp (Cyprinus carpio)

Cited by 63 publications

References 21 publications

The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals

The differential effects of cadmium exposure on the growth and survival of primary and established cells from fish and mammals

Antioxidant Defenses in Fish: Biotic and Abiotic Factors

A chemical enterocolitis model in zebrafish larvae that is dependent on microbiota and responsive to pharmacological agents

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