Effects of vitamin E on serum biochemical indexes and antioxidant capacity of<i>Megalobrama amblycephala</i>under acute high temperature stress and recovery

Zhou, Ming; Liu, Bo; Ge, Xianping; Xie, Jun; Chen, Ruli; Cui, Yanting; Wan, Jinjuan; Cui, Suli; Habte‐Tsion, Habte‐Michael

doi:10.3724/sp.j.1231.2013.38535

Cited by 2 publications

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“…The levels of cortisol were measured by the automatic chemiluminescence immunoassay analyzer MAGLUMI 1000 (Shenzhen, China) using assay kits purchased from Shenzhen New Industries Biomedical Engineering Co., Ltd, China, following a previously described method (Cui et al2013;Zhou et al 2013). Serum C3 and C4 activities were measured using the immunoturbidimetric method and the kits were purchased from Zhejiang Yilikang Biotech Co., Ltd (P.R.…”

Section: Serum Cortisol C3 and C4 Measurementmentioning

confidence: 99%

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Liu¹,

Wan²,

Ge³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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We evaluated the effect of vitamin C (Vit C) supplementation on the resistance of Megalobrama amblycephala to Aeromonas hydrophi1a infection under pH stress. Fish were randomly divided into six groups: a control group (fed with a basal diet) and five treatment groups (fed with basal diet supplemented with 33.4, 65.8, 133.7, 251.5 and 501.5 mg/kg Vit C, respectively). After 90 days, fish were exposed to combined stressors, first pH 9.5 followed by A. hydrophila infection. The results showed that 133.7 mg/kg vit C improved complement 4 (C4), anti-superoxide anion free aradical (ASAFER) and heat shock protein (HSP) 70 compared with the control group; and 251.5 mg/kg vit C enhanced complement 3 (C3), HSP60, HSP70 and HSP90 compared to the control group before stress. After pH stress and A. hydrophila infection, hemoglobin, ASAFER and HSP60, HSP70, HSP90 in the groups fed with 133.7 and 251.5 mg/kg vit C were still significantly higher, while serum cortisol in the group fed with 251.5 mg/kg vit C was lower compared to the control group 15 days after pH stress. The cumulative mortality of the control group was higher than that of the five treatment groups at 12, 24 h after A. hydrophila infection. The results of this study suggest that 133.7-251.5mg/kg vit C in a diet could have potential to stimulate immune response, and enhance resistance against high pH stress and A. hydrophila infection of Wuchang bream.

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Section: Serum Cortisol C3 and C4 Measurementmentioning

confidence: 99%

Untitled

Liu¹,

Wan²,

Ge³

et al. 2016

Turk. J. Fish. Aquat. Sci.

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Effects of dietary vitamin E supplementation on growth performance, fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) expressions in juvenile blunt snout bream Megalobrama amblycephala

Zhang

Liang

et al. 2017

Fish Physiol Biochem

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A 9-week feeding experiment was conducted to evaluate the effects of dietary vitamin E (VE) supplementation on growth performance, liver fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) genes expressions in blunt snout bream juveniles. Fish (average initial weight: 0.59 g) were fed diet supplemented with 0, 50, 100, 300 and 500 mg α-tocopherol acetate/kg in triplicates, which were found to, respectively, contain 11.2, 56.3, 114.6, 306.5 and 588.4 mg α-tocopherol/kg diet. Results showed that final weight, body weight gain and specific growth rate significantly increased with increasing dietary VE supplemented level from 11.2 to 56.3 mg/kg. When the broken-line model was employed to estimate the adequate requirement of vitamin E based on body weight gain, the optimal level was 55.5 mg/kg in diet. Hepatosomatic index value significantly decreased with incremental dietary VE levels. However, liver VE concentration showed a direct relationship with the dietary VE level. The percentages of 20:5n-3, 22:6n-3 and total n-3 long chain polyunsaturated fatty acids in liver increased with increasing dietary VE supplementation. Meanwhile, the expressions of PPAR-α, PPAR-β and PPAR-γ in liver were down-regulated by supplementation of dietary VE level from 56.3 to 588.4 mg/kg. In conclusion, supplementation of more than 55.5 mg/kg vitamin E may improve growth and increase n-3 LC-PUFA content in blunt snout bream, which is beneficial to human consumer.

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Effects of vitamin E on serum biochemical indexes and antioxidant capacity ofMegalobrama amblycephalaunder acute high temperature stress and recovery

Cited by 2 publications

References 0 publications

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Effects of dietary vitamin E supplementation on growth performance, fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) expressions in juvenile blunt snout bream Megalobrama amblycephala

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