Effects of Vitamin C or E on the Pro-inflammatory Cytokines, Heat Shock Protein 70 and Antioxidant Status in Broiler Chicks under Summer Conditions

Abstract

The present study was carried out to investigate the effects of dietary antioxidants on pro-inflammatory cytokines, heat shock protein (HSP) and antioxidant status in broiler chicks under summer conditions. A total of 162, 3-d-old broiler chicks were randomly assigned to a basal diet (CON) and the basal diet supplemented with vitamin C (200 mg/kg diet, VCD) or vitamin E (100 mg/kg, VED) until 35 day of age. All birds were exposed to summer diurnal heat stress at average daily fluctuations of temperature between 32°C to 34°C at day to 27°C to 29°C at night for the entire feeding periods. There was no significant difference in body weight, feed to gain ratio and the relative organ weight except the thymus in response to dietary vitamin C or E supplementation. However, the mRNA expression of interleukin (IL)-1β, IL-6, interferon (IFN)-γ, Toll like receptor (TLR)-4 and HSP70 in the liver of birds fed diet containing vitamin C significantly (p<0.05) decreased compared with those in birds fed basal diet. Dietary vitamin E also showed a significant (p<0.05) decrease in the mRNA expression of IL-6 and HSP70 compared with a basal diet. Total antioxidant status (TAS) in serum of birds fed vitamin C supplemented diet was significantly (p<0.05) higher with than that in birds a basal diet. Lipid peroxidation in serum and liver resulted in a significant (p<0.05) decrease in response to dietary vitamin C or E supplementation. In conclusion, dietary supplementation with antioxidant vitamins, especially vitamin C resulted in a significant decrease in the mRNA expression of pro-inflammatory cytokines and HSP70, and higher antioxidant parameters than that of birds on the basal diet under summer conditions.

Effect of Ascorbic Acid on Some Biochemical Parameters during Heat Stress in Commercial Broilers

“…The probable reason for such reports might be due to the fact that rearing broiler chickens for 5-6 weeks may not be long enough for the ascorbic acid to have an effect on muscle metabolism, which could also be the reason for present findings. Jang et al, (2014) reported that dietary supplementation with vitamin C subjected to summer HS in broilers markedly decreased (TBA value) lipid peroxidation in liver compared with the control group, is in agreement to our findings. In the present study, dietary vitamin C significantly decreased hepatic lipid peroxidation.…”

Effects of feeding cassava pulp fermented with Acremonium charticola on growth performance, nutrient digestibility and meat quality of broiler chicks

“…As a consequence, less toxin will be produced and liver will have less work in detoxifying these toxin (Mehr et al, 2007). In terms of the antioxidant property of the fungus, it might have a minimal effect on the liver weight, as Jang et al (2014) reported no effect of antioxidants (vitamins C and E) on the relative weight of liver in broiler chicks. Eventually, the lower liver weight in the present study may confirm that the toxicological effect of urea used as a nitrogen source in the fermentation process did not appear in broiler chicks (Khempaka et al, 2014;Sugiharto et al, 2016a).…”

The Effect of Stocking Density and Strain on the Performance and Physiological Adaptive Responses in Broiler Chickens

“…그러 나 닭에서 사육 밀도 증가에 따른 동물 복지에 관련된 다양 한 지표들을 조사하였지만, 많은 요인들의 상호작용 및 복 합성으로 이들 지표들의 일관성은 높지 않는 것으로 알려져 있다 (Thaxton et al, 2006). 최근에는 닭에서 사육 밀도, 추위, 고온 등과 같은 외부 스트레스 요인이 친염증 사이토카인 및 항산화 방어력에 영향을 미치는 연구 결과가 발표되었다 (Hangalapura et al, 2006;Jang et al, 2014;Kaiser et al, 2012;Kang et al, 2011). 특히 스트레스에 따른 corticosterone 수준의 증가와 물리적 스트레스 요인이 면역 작용과 직접적 인 연관성이 있다는 연구 결과가 발표되면서 염증 관련 사이 토카인이 스트레스 평가 지표로서 주목 받고 있다 (Hangalapura et al, 2006;Shini et al, 2010).…”