Effects of vitamin A overdose on rat’s organs involved in immunity and vitamin A storage

Mahassni, Sawsan Hassan; Alshaikh, Nahla

doi:10.1556/aalim.43.2014.3.12

Cited by 4 publications

(5 citation statements)

References 16 publications

(14 reference statements)

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“…This result is in agreement with Barandeh et al, (2019), who demonstrated that RA applies toxic effects on the development of the embryonic organ in a dose and time-dependent manner. But the present result was disagreement with Mahassni & Al-Shaikh, (2014) they showed no connection between oral intake of vitamin A and liver weight in rats.…”

Section: Histological Studies Of the Ra Effects On The Liver Of Adultcontrasting

confidence: 99%

The Effects of Different Concentrations of Retinoic Acid on the Histological Structure of Liver of Adult Albino Female Mice and Their Prenatal Fetuses

2020

JBAH

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Retinoic acid (RA) an active metabolite of vitamin A, plays essential signaling roles in mammalian embryonic development and adult homeostasis through concentration-dependent activity but in excess, RA is teratogenic. The present study was designed to evaluate the harmful effect of RA on the: liver tissues of the adult pregnant female albino mice and their prenatal fetuses and adult morphological and behavior changes. Forty-five pregnant adult female albino mice were divided into three groups (n=15 for each group). Group I, the control group orally received 0.1ml of olive oil. Group II and III received 25 and 50mg RA/kg B.W. respectively. RA was dissolved in 0.1ml of olive oil and orally given on 7 th , 8 th , and 9 th days of pregnancy. On the 18 th day of gestation, these females were sacrificed and the liver from mother and their prenatal were removed, weighed, and prepared for histological study. The RA treated adult group exhibited behavior changes including general fatigue and loss of appetite, in addition to the morphological changes which included weight loss, change in normal red eye color towards black, and redness in the mouth and chin area associated with loss of hair (fur) around this area. These observations were showed to be more severe in the 50 mg RA group. The results showed that treatment with 25 and 50mg RA caused no significant decrease in the adult liver weight, while in their prenatal fetuses 50mg RA caused a higher significant decrease in the liver weight, but 25 mg RA caused no significant effects on this weight. Treated with 50mg/kg RA caused a highly significant increase in the number of aborted and dead fetuses as compared with other groups. The results of the histological study showed that treatment with both concentrations of RA caused several degrees of the liver damage with disrupted the normal architecture pattern along with hepatocellular steatosis, hypertrophy, and inflammation. In conclusions, RA caused variable degrees of degeneration and destruction of the liver tissues of adult female (mother) and their prenatal. The most important effects of RA are causing hepatocellular steatosis, hypertrophy, and inflammation, and when RA giving during the critical periods of embryonic development, caused harmful effects on the developing liver, therefore treatment with RA should be avoided at any stage of gestation.

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Section: Histological Studies Of the Ra Effects On The Liver Of Adultcontrasting

confidence: 99%

The Effects of Different Concentrations of Retinoic Acid on the Histological Structure of Liver of Adult Albino Female Mice and Their Prenatal Fetuses

2020

JBAH

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“…It is believed that excessive vitamin A can disrupt the balance and activation of immune cells, thus interfering with the intricate network of immune responses. This notion is supported by studies conducted on rats, where the administration of vitamin A significantly higher than their dietary requirements (8,000 and 15,000 IU/kg body weight) had a stimulatory effect on total white blood cell and neutrophil counts, while inhibiting basophil and total lymphocyte counts (Mahassni and Al-Shaikh, 2013).…”

Section: Effect Of Hypervitaminosis a On Immune Functionmentioning

confidence: 82%

The role of vitamin A in non-ruminant immunology

Shastak

Pelletier

2023

Front. Anim. Sci.

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Vitamin A (retinol) is an essential micronutrient with a crucial role in the immune system of non-ruminant animals, such as swine and poultry. It includes three chemical compounds with distinct properties and functions in the body: retinol, retinal, and retinoic acid. In monogastric feed, vitamin A is primarily present in the form of retinyl esters. The metabolism of dietary vitamin A esters involves their conversion to retinol, which is then transported to different tissues and cells for further metabolism into active forms such as retinoic acid. These active forms of vitamin A have been found to play a crucial role in regulating both innate and adaptive immune responses. Specifically, they are involved in the differentiation, proliferation, and function of immune cells such as T and B lymphocytes, as well as dendritic cells. Vitamin A deficiency can lead to impaired cellular immunity, reduced antibody production, and consequently an increased susceptibility to infections. In swine and poultry, hypovitaminosis A can also affect gut-associated lymphoid tissues, leading to gut-related health problems and compromised growth performance. On the other hand, vitamin A supplementation has been shown to have immunomodulatory effects on non-ruminant immune responses. By administering or supplementing retinol, immune cell proliferation, antibody production, and cytokine secretion can be enhanced, which can ultimately result in improved immune function and disease resistance. Therefore, vitamin A has potential applications as an immuno-micronutrient for improving health and preventing diseases in swine and poultry. However, the optimal dosage and timing of vitamin A supplementation need to be carefully determined based on the specific requirements of different non-ruminant species and their production stages. Overall, a better understanding of the role of vitamin A in non-ruminant nutritional immunology could have significant implications for animal health and productivity and could inform the development of effective dietary strategies to optimize immune function and prevent diseases in swine and domestic fowl. This review paper aims to offer valuable insights into the role of vitamin A in the nutritional immunology of non-ruminants while also emphasizing the current gaps in knowledge and potential areas for further research.

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“…Histological study: the mandibular condyles of each rat were dissected out and were fixed in10% neutral buffered formalin for 24h .After fixation, specimens were decalcified in 10% trichloroacetic acid, washed in tap water over night and then dehydrated in ascending concentrations of alcohol, cleared in xylene and then infiltrated and embedded in low melting point (56 o C) paraffin wax (21).Tissue blocks were cut coronal at a 5 µm thickness with a microtome (22).Sections were stained with Hematoxylin and Eosin stain (H&E) for general examination (21) . Quantitative determination of serum vitamin A concentrations: (7,23) At the end of the experimental period and before euthanization, blood samples were collected from the orbital plexus of rats. This technique is called "orbital venous plexus bleeding".…”

Section: Study Samplementioning

confidence: 99%

“…The absorption of vitamin A occurs in the intestine with the help of lipids .It is stored mainly in the liver, kidney, and adipose tissues (7).…”

Section: Introductionmentioning

confidence: 99%

Effect of Hypervitaminosis a on Growth of Mandibular Condyle in Rats

halawa

Abdelmajeed

El-Adway

et al. 2023

Alexandria Dental Journal

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BACKGROUND: Vitamin A is present in our food such as meat, dairy products, and vegetables. A control balanced diet maintains our nutritional needs of vitamin A. Over consumption of products as well as supplementations with vitamin A increases the risk of hypervitaminosis A. Hypervitaminosis A has been associated with thinning of the long bones and reducing of bone formation OBJECTIVES: to investigate the effect of over as well as optimum doses of vitamin A on growth of mandibular condyle in Wister albino rats. MATERIALS AND METHODS: Eight healthy (2month old age) male Wister albino rats weighting (274 -375grams) were used in present study. The rats were randomly divided into 2 equal group, study and control group .In the control group; rats fed a standard diet containing an optimum dose of vitamin A (12 IU/g of each pellet). In the study group, rats fed a standard diet supplemented with overdose of vitaminA (1700 IU /g of each pellet). After 2months, all rats were euthanized. Blood samples were collected from all rats the day after administering the last dose of vitamin and the concentration of vitamin A in serum was determined for all samples. The condyles of each rat of all groups were dissected out and processed for histological study and histomorphometric analysis. RESULTS: In the study group, the histological examination revealed marked reduction in thickness of hypertrophic zones and increase in the width of chondroblastic zones of condylar cartilage. Bone trabeculae were thin with irregular outline. The subchondral bone showed marked bone resorption with numerous osteoclasts in Howship's lacunae as compared with the control group. CONCLUSIONS: findings from the present study have indicated that, hypervitaminosis A increases resorption of the bone and diminishes endochondral bone growth.

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Effects of vitamin A overdose on rat’s organs involved in immunity and vitamin A storage

Cited by 4 publications

References 16 publications

The Effects of Different Concentrations of Retinoic Acid on the Histological Structure of Liver of Adult Albino Female Mice and Their Prenatal Fetuses

The Effects of Different Concentrations of Retinoic Acid on the Histological Structure of Liver of Adult Albino Female Mice and Their Prenatal Fetuses

The role of vitamin A in non-ruminant immunology

Effect of Hypervitaminosis a on Growth of Mandibular Condyle in Rats

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