Effects of veratrine and paeoniflorin on isolated mouse vas deferens

Chen, Y.F.; Lin, Yu Tzu; Tan, Tzu-Wei; Tsai, Huei-Yann

doi:10.1078/0944-7113-00122

Cited by 4 publications

(4 citation statements)

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“…Na V 1.6 +/+ ) but not Na V 1.6‐null mice (i.e. Na V 1.6 −/− ), similar to the contractions induced by veratrine (a mixture of alkaloid esters, including veratridine, cevadine, cevine, cevadilline, and sabadine) detected by Chen et al. (2002).…”

Section: Discussionmentioning

confidence: 60%

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na⁺ currents, Na_V1.6, in murine vas deferens myocytes

Zhu¹,

Wassall

Takai³

et al. 2009

British J Pharmacology

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Background and purpose:The effects of veratridine, an alkaloid found in Liliaceae plants, on tetrodotoxin (TTX)-sensitive voltage-gated Na + channels were investigated in mouse vas deferens. Experimental approach: Effects of veratridine on TTX-sensitive Na + currents (INa) in vas deferens myocytes dispersed from BALB/c mice, homozygous mice with a null allele of NaV1.6 (NaV1.6 -/-) and wild-type mice (NaV1.6 +/+ ) were studied using patch-clamp techniques. Tension measurements were also performed to compare the effects of veratridine on phasic contractions in intact tissues. Key results: In whole-cell configuration, veratridine had a concentration-dependent dual action on the peak amplitude of INa: INa was enhanced by veratridine (1-10 mM), while higher concentrations (Ն30 mM) inhibited INa. Additionally, two membrane current components were evoked by veratridine, namely a sustained inward current during the duration of the depolarizing rectangular pulse and a tail current at the repolarization. Although veratridine caused little shift of the voltage dependence of the steady-state inactivation curve and the activation curve for INa, veratridine enhanced a non-inactivating component of INa. Veratridine caused no detectable contractions in vas deferens from NaV1.6 -/-mice, although in tissues from NaV1.6 +/+ mice, veratridine (Ն3 mM) induced TTX-sensitive contractions. Similarly, no detectable inward currents were evoked by veratridine in NaV1.6 -/-vas deferens myocytes, while veratridine elicited both the sustained and tail currents in cells taken from NaV1.6 +/+ mice. Conclusions and implications:These results suggest that veratridine possesses a dual action on INa and that the veratridineinduced activation of contraction is induced by the activation of NaV1.6 channels.

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Section: Discussionmentioning

confidence: 60%

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na⁺ currents, Na_V1.6, in murine vas deferens myocytes

Zhu¹,

Wassall

Takai³

et al. 2009

British J Pharmacology

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“…We have known that neurotransmitters release in the nervous endings required extracellular calcium influx via calcium channel [12, 13]. In previous studies, paeoniflorin inhibited calcium influx and blocked intracellular calcium movement [15-20]. We would suggest that the effects of paeoniflorin on the central nervous system might mediate by the inhibition of intracellular calcium flux.…”

Section: Discussionmentioning

confidence: 97%

“…The L-type voltage-sensitive calcium channel involves in noradrenaline release in the rat brain [14]. From previous studies, paeoniflorin inhibited calcium influx and blocked intracellular calcium movement [15-20]. The antinociceptive responses of L-arginine were decreased by the L-type calcium channel antagonists, diltiazem or verapamil [21].…”

Section: Introductionmentioning

confidence: 99%

In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain

Lin¹,

Huang²,

Tsai³

et al. 2019

BioMedicine

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Background: Paeoniflorin (PF) possesses several effects such as analgesic, the anti-spasmodic effect on smooth muscle. It protects the cardiovascular system and reveals the neuroprotective effect on cerebral ischemia. Monoamine system has been identified to have complex regulatory effects in pain signaling. There are no reports regarding the impact of PF on monoamine levels in the rodent brain by microdialysis. In this study, the effects of PF on monoamines and their metabolites in the rodent brain using in vivo microdialysis and in vitro high performance liquid chromatography (HPLC) analysis. Methods: Male S.D. rats were anesthetized, fixed onto the stereotaxic instrument to identify the positions of corpus striatum and cerebral cortex. Drilled a hole in the skull of anesthetic rats and proceeded microdialysis, and gave PF (100 μg, i.c.v.). Collected the dialysate and the concentration of monoamines and their metabolites in dialysate and analyzed with HPLC-ECD. Male ICR mice were administered with PF (96 μg, i.c.v.) and with Ringer solution as a control. After 20 mins of administration, the mice were cut off the brain immediately and separated into eight regions according to the method of Glowinski. Added extraction solution to each region, homogenized and extracted for further procedure. The extract was centrifuged, sucked the transparent layer and centrifuged once more. The transparent layer was filtered with a 0.22 μm nylon filter and analyzed with HPLC-ECD (electrochemical detection). Results: PF increased the content of DOPAC and NE in the cortex, and increased the content of NE and decreased the content of 5-HT in the medulla of the homogenized mice brain tissue. By microdialysis, PF increased the content of DOPAC and 5-HIAA in anesthetic rat cortex and expanded the content of DOPAC, HVA, and 5-HIAA in anesthetic rat striatum. Conclusions: It reveals that PF could activate the release of monoamines and increase their metabolites in the rodent brain.

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“…Our previous study (Luo et al, 2009) confirmed that in vitro, paeoniflorin could also relax HC rabbit SO muscle rings. Chen et al (2002) proved that paeoniflorin inhibited tetanic contractions of vas deferens, epididymis and prostate smooth muscle of rats, inhibited in vitro intestine and in vivo stomach movement of cavies and rats, as well as uterine smooth muscle of rats, and could antagonize oxytocin-induced contraction. Also, paeoniflorin showed synergism with methanol extract of licorice (FM100).…”

Section: +mentioning

confidence: 99%

Influence of paeoniflorin on intracellular calcium ion concentration in the sphincter of Oddi of hypercholesterolemic rabbits

Wang¹,

Wang²,

Liu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. This study aimed to investigate the influence of hypercholesterolemia (HC) on intracellular calcium ion concentration in the sphincter of Oddi (SO) of rabbits and the influence of paeoniflorin on intracellular calcium ion concentration in the hypercholesterolemic rabbit SO. Sixteen purebred New Zealand rabbits were randomly divided into two groups: the control group and the HC model group (8 rabbits in each group). The control group was fed standard diet. The HC group was fed standard diet plus cholesterol for a total of 8 weeks to induce and establish the rabbit HC model. The SO segment of HC rabbits was taken and enzyme treated to obtain SO cells. After primary culture, immunohistochemical analysis was performed. Fluo-3/AM was used to load SO cells, and then intracellular calcium ion concentration was determined by confocal microscopy. Intracellular calcium ion in the SO of the HC group was higher than that of the normal group; intracellular calcium ion in the HC rabbit SO of the paeoniflorin group was lower than that of the control group, where the paeoniflorin effect was greater with higher concentrations. High cholesterol caused an increase in intracellular calcium ion concentration in the rabbit SO, and paeoniflorin can reduce intracellular calcium ion concentration in the HC rabbit SO in a concentration-dependent manner.

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Effects of veratrine and paeoniflorin on isolated mouse vas deferens

Cited by 4 publications

References 20 publications

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na⁺ currents, Na_V1.6, in murine vas deferens myocytes

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na⁺ currents, Na_V1.6, in murine vas deferens myocytes

In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain

Influence of paeoniflorin on intracellular calcium ion concentration in the sphincter of Oddi of hypercholesterolemic rabbits

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Effects of veratrine and paeoniflorin on isolated mouse vas deferens

Cited by 4 publications

References 20 publications

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na+ currents, NaV1.6, in murine vas deferens myocytes

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na+ currents, NaV1.6, in murine vas deferens myocytes

In vivo microdialysis and in vitro HPLC analysis of the impact of paeoniflorin on the monoamine levels and their metabolites in the rodent brain

Influence of paeoniflorin on intracellular calcium ion concentration in the sphincter of Oddi of hypercholesterolemic rabbits

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Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na⁺ currents, Na_V1.6, in murine vas deferens myocytes

Actions of veratridine on tetrodotoxin‐sensitive voltage‐gated Na⁺ currents, Na_V1.6, in murine vas deferens myocytes