Effects of Veratrilla baillonii Extract on Hepatic Gene Expression Profiles in Response to Aconitum brachypodum-Induced Liver Toxicity in Mice

Abstract

This manuscript was aimed to explore the hepato-protective effect of water extract of Veratrilla baillonii Franch. (Gentianaceae) (WVBF) on serious hepatic toxicity induced in mice treated with Aconitum brachypodum Diels (Ranunculaceae) at transcriptome level. The physiological and pathological symptoms were evaluated as the markers for hepato toxicity induced by A. brachypodum Diels (CFA) extracted compounds. Moreover, gene chip method was used to compare and investigate the gene expression level of WVBF on CFA induced-liver toxicity to identify the potential target of WVBF and CFA on liver. The results showed that WVBF had a significant detoxification effect on CFA-induced acute hepatic toxicity. There were 130 genes with lower expression and 124 genes expressed at higher rate in CFA treated group as compared with normal control group, while there are 67 genes down-regulated and 74 genes up-regulated in WVBF treated group in comparison with CFA treated group. WVBF could attenuate CFA-induced liver damage in mice through regulating oxidative stress, inflammatory injury and cell apoptosis/necrosis pathways. On the other hand, WVBF and CFA may have potential synergetic effects on the target genes of certain diseases such as inflammation, cancer and diabetes.

Veratrilla baillonii Franch Could Alleviate Lipid Accumulation in LO2 Cells by Regulating Oxidative, Inflammatory, and Lipid Metabolic Signaling Pathways

“…S20140710) were deposited at the Herbarium situated in the College of Pharmaceutical Sciences at South-Central University for Nationalities, Wuhan, Hubei, P. R. China. The WVBF was extracted as described previously (Ge et al, 2016;Yu et al, 2016;Li et al, 2019). Briefly, WVBF was prepared by extracting the plant (200 g) crushed into small pieces.…”

“…V. baillonii, a traditional folk medicine, has been widely used in the treatment of hepatitis-induced jaundice and drug-induced hepatitis for decades (Olennikov et al, 2015;He et al, 2015). The water extract of V. baillonii (WVBF), which contain flavonoids, iridoid glycosides, and other substances (Vaidya et al, 2009;Ge et al, 2016;Yu et al, 2016;Zhang S. R. et al, 2018;Li et al, 2019). WVBF has significant protective effects on oxidative stress-induced liver injury (Dai et al, 2018), diabetic liver injury (Lian et al, 2010), and drug liver toxicity (Huang et al, 2016).…”

Veratrilla baillonii Franch Ameliorates Diabetic Liver Injury by Alleviating Insulin Resistance in Rats

“…Our previous work has identified that WVBF could attenuate hepatic toxicity induced by Aconitum brachypodum Diels (Ranunculaceae) in vivo and in vitro (Ge et al, 2016;Yu et al, 2016;Dai et al, 2018b;Cheng et al, 2019;Huang et al, 2019;Li et al, 2019). Further study revealed that the anti-diabetic and hepatic protection activities of WVBF is mediated by IRS-1/PI3K/AKT signal pathway in T2DM db/db mice.…”

“…Gentiopicroside, sweroside, and swertiamarin are the characteristic components of the medicinal plants of Gentianaceae used in the practice of traditional Chinese Medicine. Our previous study identified that WVBF are rich in sweroside, swertiamarin, gentiopicroside (GP) (Ma et al, 2018), which can exert anti-hepatotoxic and hepatoprotective effects (Dai et al, 2018a;Li et al, 2019). Among many Chinese herbal extracts with hepatoprotective activity, the extracts of gentian plants endow uniquely advantages, including hepatoprotection and rescue of liver injury (Zhao et al, 2012).…”

“…The content of sweroside and gentiopicroside from WVBF was 0.36 and 3.75%, respectively (Ma et al, 2018). Veratrilla baillonii Franch (V. baillonii), a member of Gentianaceae family, is characterized by anti-toxic and liver protection properties (Li et al, 2019), and used in the intervention of drug-induced hepatitis and hepatitis-induced jaundice (Olennikov et al, 2015;Zhang et al, 2015). The water extract of V. baillonii (WVBF) has significant protective effects on oxidative stress-induced liver injury, diabetic liver injury, and drug induced liver toxicity (Lian et al, 2010;Huang et al, 2016;Huang et al, 2020).…”