Effects of various polysaccharides (alginate, carrageenan, gums, chitosan) and their combination with prebiotic saccharides (resistant starch, lactosucrose, lactulose) on the encapsulation of probiotic bacteria Lactobacillus casei 01 strain

Ta, Linh Phuong; Bujna, Erika; Antal, Otília; Ladányi, Márta; Juhász, Réka; Szécsi, Anett; Kun, Szilárd; Sudheer, Surya; Gupta, Vijai Kumar; Nguyen, Quang D.

doi:10.1016/j.ijbiomac.2021.04.170

Cited by 79 publications

(38 citation statements)

References 50 publications

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“…When bacteria are mixed with alginate the bacteria are trapped within the polymerized matrix following the introduction of calcium. This technique has been used to protect probiotics for passage through acidic environments of the gastrointestinal track [18][19][20][21]. Lactobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

et al. 2022

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Francisella tularensis is the etiologic agent of tularemia and a Tier I Select Agent. Subspecies tularensis (Type A) is the most virulent of the four subspecies and inhalation of as few as 10 cells can cause severe disease in humans. Due to its niche as a facultative intracellular pathogen, a successful tularemia vaccine must induce a robust cellular immune response, which is best achieved by a live, attenuated strain. F. tularensis strains lacking lipopolysaccharide (LPS) O-antigen are highly attenuated, but do not persist in the host long enough to induce protective immunity. Increasing the persistence of an O-antigen mutant may help stimulate protective immunity. Alginate encapsulation is frequently used with probiotics to increase persistence of bacteria within the gastrointestinal system, and was used to encapsulate the highly attenuated LVS O-antigen mutant WbtIG191V. Encapsulation with alginate followed by a poly-L-lysine/alginate coating increased survival of WbtIG191V in complement-active serum. In addition, BALB/c mice immunized intraperitoneally with encapsulated WbtIG191V combined with purified LPS survived longer than mock-immunized mice following intranasal challenge. Alginate encapsulation of the bacteria also increased antibody titers compared to non-encapsulated bacteria. These data suggest that alginate encapsulation provides a slow-release vehicle for bacterial deposits, as evidenced by the increased antibody titer and increased persistence in serum compared to freely suspended cells. Survival of mice against high-dose intranasal challenge with the LVS wildtype was similar between mice immunized within alginate capsules or with LVS, possibly due to the low number of animals used, but bacterial loads in the liver and spleen were the lowest in mice immunized with WbtIG191V and LPS in beads. However, an analysis of the immune response of surviving mice indicated that those vaccinated with the alginate vehicle upregulated cell-mediated immune pathways to a lesser extent than LVS-vaccinated mice. In summary, this vehicle, as formulated, may be more effective for pathogens that require predominately antibody-mediated immunity.

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Section: Introductionmentioning

confidence: 99%

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

et al. 2022

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“…In accordance, application of novel nano-vehicle for delivery of probiotics strains increased their survival and stability along gastrointestinal tract (GIT) 17 . Additionally, entrapping of probiotics in to a nano system has many advantages as to maintain probiotics stability, deliver a barrier to protect them from damage, to isolate bacteria from their environmental conditions, to provide a carrier with a high probiotics load, to allow controlled and continuous probiotics release, to enable probiotic adherence and prolong residence time 24 , 25 . All these functions augmented their oral colon-targeted therapy.…”

Section: Discussionmentioning

confidence: 99%

Probiotics-loaded nanoparticles attenuated colon inflammation, oxidative stress, and apoptosis in colitis

Al-Kushi

Abdelfattah‐Hassan

Eldoumani

et al. 2022

Sci Rep

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Promising therapy is needed for treating inflammatory bowel diseases (IBD) to overcome current treatment that inefficient and associated with unnecessary health risks. Recently, the concept of incorporating natural products into nanocarriers has been intended as a promising therapy for treating IBD via modulating their stability and bioavailability. Thus, we aimed to explore the potential alleviating effects of dietary nano-supplement combined with bacillus strains (Bacillus amyloliquefaciens; BANPs) in colitis model. Rats were orally gavaged by 5% DSS and the efficacy and mechanistic actions of BANPs were evaluated by assessing the severity of clinical signs and inflammatory and apoptosis response, histopathological and immunohistochemistry examination in colonic tissues. The severity of clinical signs was successfully alleviated and fecal Lcn-2 levels, an important colitic marker, were decreased in BANPs then free BA treated groups. In contrast, inflammatory markers overexpression IL-6, IL-1β, TNFα, COX-2, and iNOS in the colitic group were reduced more prominently in BANPs treated group, unlike free BA. The amelioration of BANPs to colon injury was also correlated with oxidative stress suppression along with restoring total antioxidant capacity. Interestingly, BANPs treatment modulated apoptotic markers as proved by downregulation of cytochrome c, and caspase-3 and upregulation of Bcl-2 and Bax than free BA. The severity of the histopathological alterations in the colon was greatly reduced in BANPs than free BA groups. Remarkably, over-expression of ki67 and IL-6 in colonic tissues were suppressed in BANPs group. These findings together highlighted the beneficial efficacy of BANPs in IBD treatment which are evidenced by colonic inflammation alleviation. Taken together, these results recommend that BANPs is a promising agent that encourages its possible therapeutic role in colitis treatment.

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“…Therefore, one of the critical factors in the application of probiotic products is the use of the delivery system to protect viable cells during transmission in the human digestion system. According to Ta et al (2021), Lactobacillus casei 01 was well encapsulated into gellan gum-xanthan gum, and nonspherical capsules were formed, which increased bacterial viability [65]. Nag et al (2011) reported sodium caseinate mixed with gellan gum is an ideal wall material for Lactobacillus casei encapsulation [66].…”

Section: Application Of Gums In Other Industrymentioning

confidence: 99%

Novel Approaches for Encapsulation of Plant Probiotic Bacteria with Sustainable Polymer Gums: Application in the Management of Pests and Diseases

Riseh

Tamanadar

Pour

et al. 2022

Advances in Polymer Technology

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The unique attributes, biodegradability, biocompatibility, perfect accessibility, and low production costs led to the use of natural gums in a different section of our lives. Among them, we can mention gums obtained from microorganisms (xanthan gum and gellan gum), plant tissues (Arabic gum and gum tragacanth), seeds (konjac gum and guar gum), seaweeds (alginates, agar gum, and carrageenans). Gums have essential applications in the medical and pharmaceutical, food, biotechnology, and critical agricultural industries. Encapsulation is one of the new methods to increase the stability of bioactive compounds during processing and storage. Encapsulation technology using natural gums is a new way to improve the performance of microbial agents in various sciences, especially agriculture, which represents a bright future in this field.

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Effects of various polysaccharides (alginate, carrageenan, gums, chitosan) and their combination with prebiotic saccharides (resistant starch, lactosucrose, lactulose) on the encapsulation of probiotic bacteria Lactobacillus casei 01 strain

Cited by 79 publications

References 50 publications

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

Alginate microencapsulation of an attenuated O-antigen mutant of Francisella tularensis LVS as a model for a vaccine delivery vehicle

Probiotics-loaded nanoparticles attenuated colon inflammation, oxidative stress, and apoptosis in colitis

Novel Approaches for Encapsulation of Plant Probiotic Bacteria with Sustainable Polymer Gums: Application in the Management of Pests and Diseases

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