Effects of Various Levels of Luteinizing Hormone and Caprine Follicular Fluid on In Vitro Embryo Production of Shami Goat

Abstract

In the current study, the hypothesis of the effects of luteinizing hormone (LH) and follicular fluid (FF) derived from follicles of varying size on in vitro embryo production of the Shami goat breed were tested. The caprine follicular fluid (cFF) was obtained from healthy female’s ovaries by aspiration method and classified into two main classes (follicles with a diameter of ≤ 2mm and ≥3mm). The resulting cFF was added to the culture medium TCM-199 through six Treatments (A, B and C with a source of follicle size of ≤ 2mm; D, E and F with a source of size of ≥3mm). LH was added only to four of the previous Treatments with the levels of 50 µg ml-1 (B and E) and100 µg ml-1 (C and F). Results of the study showed that the oocytes incubated in Treatment F achieved a clear superiority (p=0.001) in the rates of maturation (87.0%), fertilization (80.0%) and cleavage (82.3%). The oocytes incubated in the same Treatment (F) continued to outperform (p= 0.006) by achieving the best rates across cleavage stages at 2-16 cell (16%; the lower value of arrest) and blastocyst (42%). Significant differences (P=0.03) were observed among the rates of Type 1embryos (the highest rate: 45.3%; Treatment F) and Type 3 embryos (the highest rate: 45.1%; Treatment A). No significant differences were observed in the rates of morula and Type 2 embryos. It is advised to add 15% of the cFF derived from follicles with a diameter of ≥3mm and 100 µg of LH ml-1 in the maturation media to obtain higher rates of maturation and cleavage of goat oocytes.

Gonadotropin supplementation improved in vitro developmental capacity of Egyptian goat oocytes by modulating mitochondrial distribution and utilization

“…By altering the nutritional environment to boost the energy available for the oocyte to support future development in fertilization and cleavage, LH may also improve the oocyte IVM. Enhanced glycolysis, together with increased mitochondrial glucose oxidations and increased tricarboxylic acid (TCA) cycle activity within cumulus cell-enclosed oocytes, could be used to demonstrate the net metabolic effect of LH exposure during the maturation stage (Mardenli et al, 2021). Overall, supplementing the IVM medium with a high concentration of hCG (20 IU/mL) improved goat oocytes' maturation and fertilization rates by modulating mitochondrial distribution and enhancing their activity.…”

Evaluation of the In Vitro Embryo Production of Farm Animals Under the Circumstances of Embryo Transfer Technology

“…No doubt, the IVEP technology of farm animal embryos is characterized by high costs and sophisticated laboratory tools, as is the production of embryos in humans. However, the requirements of the breeding plans are mostly aimed at producing genetically superior individuals to accelerate genetic improvement and reach a high ceiling of production [9][10][11][12][13][14]. Perhaps this is closely related to animal breeds of high productivity and very desirable by breeders, but in the end, the factors that govern the success of IVEP efficiency are the transfer of these embryos, the rates of implantation and the conception rates.…”