Effects of Various Doses of Estradiol on Chlamydial Genital Infection in Ovariectomized Guinea Pigs

Pasley, James N.; Rank, Roger G.; Hough, Aubrey J.; Cohen, Cynthia; Barron, Almen L.

doi:10.1097/00007435-198501000-00003

Cited by 32 publications

(10 citation statements)

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“…Even during intermediate estrogen phases, when the progesterone concentra- on May 9, 2018 by guest http://iai.asm.org/ tion was fluctuating up and down, i.e., in the diestrous and proestrous stages, the epithelial cells were still susceptible to chlamydial infection and large numbers of well-formed inclusions were observed in the monolayers. These findings correlate with data obtained from the guinea pig model, where the prolongation and severity of chlamydial infection was higher in the animals treated with estradiol than in untreated animals and the animals receiving progesterone failed to show any clinical signs of chlamydiosis (22,23). In the studies involving primary human endometrial epithelial cell cultures, it was shown that the concentration of exogenously supplemented estrogen is critical and needs to be similar to concentrations in the normal human physiological range of ca.…”

Section: Discussionsupporting

confidence: 85%

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

et al. 2003

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Previous studies have demonstrated that female reproductive hormones influence chlamydial infection both in vivo and in vitro. Due to the reduced availability of human genital tissues for research purposes, an alternative hormone-responsive model system was sought to study chlamydial pathogenesis. Mature female swine eliminated from breeding programs were selected as the animals of choice because of the similarity of a sexually transmitted disease syndrome and sequelae in swine to a disease syndrome and sequelae found in humans, because of the near identity of a natural infectious chlamydial isolate from swine to Chlamydia trachomatis serovar D from humans, and because a pig's epithelial cell physiology and the mean length of its estrous cycle are similar to those in humans. Epithelial cells from the cervix, uterus, and horns of the uterus were isolated, cultivated in vitro in Dulbecco's minimum essential medium-Hanks' F-12 (DMEM-F-12) medium with and without exogenous hormone supplementation, and analyzed for Chlamydia suis S-45 infectivity. The distribution of chlamydial inclusions in swine epithelial cells was uneven and was influenced by the genital tract site and hormone status. This study confirmed that, like primary human endometrial epithelial cells, estrogen-dominant swine epithelial cells are more susceptible to chlamydial infection than are progesterone-dominant cells. Further, the more differentiated luminal epithelial cells were more susceptible to infection than were glandular epithelial cells. Interestingly, chlamydial growth in mature luminal epithelia was morphologically more active than in glandular epithelia, where persistent chlamydial forms predominated. Attempts to reprogram epithelial cell physiology and thereby susceptibility to chlamydial infection by reversestage, exogenous hormonal supplementation were unsuccessful. Freshly isolated primary pig epithelial cells frozen at ؊80°C in DMEM-F-12 medium with 10% dimethyl sulfoxide for several weeks can, after thawing, reform characteristic polarized monolayers in 3 to 5 days. Thus, primary swine genital epithelia cultured ex vivo appear to be an excellent cell model for dissecting the hormonal modulation of several aspects of chlamydial pathogenesis and infection.

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Section: Discussionsupporting

confidence: 85%

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

et al. 2003

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“…The apparent protective effects of progesterone against ascending infections have been explained by the luteal-type cervical mucus as well as by the inactive endometrium induced by this hormone. In support of these findings, Barron et al [20] reported that guinea-pigs infected with C. psittaci GPIC and treated with oestrogendominant oral contraceptives had more cases of hydrosalpinx than the controls, while Pasley et al [21] found no effect of progesterone on the pathogenesis of the infection. However, Tuffrey et al [7] have shown that the human serovars of C. trachomatis can induce a more severe genital infection in mice pretreated with progesterone.…”

Section: Discussionmentioning

confidence: 90%

Factors influencing the induction of infertility in a mouse model of Chlamydia trachomatis ascending genital tract infection

Pal

Wu²,

Peterson³

et al. 1998

Journal of Medical Microbiology

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In women, infections due to Chlamydia trachomatis frequently result in long-term sequelae including chronic abdominal pain, ectopic pregnancy and infertility. In an attempt to characterise the pathogenesis of the infection, female C3H (H-2k) mice were inoculated intravaginally with different doses of C. trachomatis and then mated with proven male breeder mice. The inoculated mice developed a broad spectrum of clinical manifestations ranging from infertility to asymptomatic shedding. The dose inducing infertility in 50% of the mice was c. lo5 inclusion-forming units of C. trachomatis. In another group of mice sampled at intervals after intravaginal inoculation, C. trachomatis was recovered from the upper genital tract starting at 24 h after infection. A higher percentage of animals infected during the luteal phase of the oestrous cycle had positive cultures from the middle and upper genital tract than when mice were inoculated during the follicular phase. These results indicate that rapid therapeutic intervention is required to avoid the sequelae resulting from C. trachomatis genital infection, and suggest that hormonal factors play a role in the pathogenesis of the disease.

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“…Bryant et al . : Raloxifene pharmacology genital infections induced by estrogen in guinea pigs [43] . Raloxifene distinguishes itself from other compounds that are used to antagonize estrogen in the uterus, in that raloxifene produces only a very mild increase in uterine wet weight when administered alone [27] .…”

Section: Effects Of Raloxifene On Gonadal Tissuementioning

confidence: 99%

A pharmacological review of raloxifene

et al. 1996

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Effects of Various Doses of Estradiol on Chlamydial Genital Infection in Ovariectomized Guinea Pigs

Cited by 32 publications

References 0 publications

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

Primary Cultures of Female Swine Genital Epithelial Cells In Vitro: a New Approach for the Study of Hormonal Modulation of Chlamydia Infection

Factors influencing the induction of infertility in a mouse model of Chlamydia trachomatis ascending genital tract infection

A pharmacological review of raloxifene

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