Effects of various concentrations of glycerol on post-thaw motility and velocity of human spermatozoa

Pilikian, S.; Czyba, J. C.; Guérin, J. F.

doi:10.1016/0011-2240(82)90135-3

Cited by 12 publications

(3 citation statements)

References 19 publications

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Section: Introductionmentioning

confidence: 99%

“…Especially, boar spermatozoa are highly susceptible to glycerol (Almilid & Johnson 1988). In fact, the final concentration of glycerol for cryopreserving spermatozoa in cattle (Wiggin & Almquist 1975), rams (Fiser & Fairfull 1984) and humans (Pilikian et al 1982) is 10-11, 4-6 and above 5%, respectively. In pigs, it has been reported that the addition of glycerol improves motility (glycerol 0%; 12%, glycerol 3%; 34%) of frozen-thawed spermatozoa (Almilid & Johnson 1988;Fiser & Fairfull 1990;Johnson et al 2000), but more than 4% glycerol affects the membrane integrity and reduces the fertilization rate after artificial insemination (Wilmut & Polge 1974).…”

Section: Introductionmentioning

confidence: 99%

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Improved conception rates in sows inseminated with cryopreserved boar spermatozoa prepared with a more optimal combination of osmolality and glycerol in the freezing extender

Okazaki¹,

Abe²,

Shimada³

2009

Animal Science Journal

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Cryoprotectant agents (CPAs) are added in freezing extenders to prevent intracellular ice crystal formation. However, it has been reported that high dose of CPAs confer toxicity on spermatozoa. Recently, the reduction of intracellular water by a high osmolality solution has also resulted in the suppression of ice crystal formation in spermatozoa, suggesting that the optimal combination of glycerol concentration and freezing extender osmolality could contribute to the development of effective sperm cryopreservation techniques. In this study, we investigated the motility, membrane and acrosomal integrity of frozen-thawed boar spermatozoa treated with freezing extender (NSF) of varying osmolalities (300, 400, 500 mOsm/kg) and final concentrations of glycerol (0.5, 1, 2, 3%). The spermatozoa that were treated at 400 mOsm/kg and 2% glycerol showed significantly higher rates of motility and membrane integrity compared with those in other treatment groups. In addition, the conception and implantation rates of swine artificially inseminated with spermatozoa frozen by the novel freezing extender (conception; 79%, implantation; 57.5%) were significantly higher than those of frozen-thawed spermatozoa treated in the conventional NSF (300 mOsm/kg, 3% glycerol) (conception; 29%, implantation; 33.8%). From these results, we concluded that the novel hyperosmotic (400 mOsm/kg) and low-glycerol (final concentration 2%) freezing extender is beneficial for the cryopreservation of boar spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved conception rates in sows inseminated with cryopreserved boar spermatozoa prepared with a more optimal combination of osmolality and glycerol in the freezing extender

Okazaki¹,

Abe²,

Shimada³

2009

Animal Science Journal

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“…Despite the success of cryopreservation of spermatozoa in assisted reproductive technology, the freezing-thawing process is undoubtedly associated with alterations of sperm quality. The impairment the most often described in the literature is decreased motility (Pilikian et al, 1982;Serafini et al, 1986;Smith and Steinberger, 1973). The main morphological abnormalities reported after freezing-thawing are coiled tails (Critser et al, 1988), acrosomal defects (Cross and Hanks, 1991;Royere et al, 1996) and damage to membrane structures (Check et al, 1991;Royere et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Sperm vacuoles are not modified by freezing–thawing procedures

Gatimel¹,

Léandri²,

Parinaud³

2013

Reproductive BioMedicine Online

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Since the development of the motile sperm organellar morphology examination (MSOME) in 2001 for observing the cephalic vacuoles at high magnification, no study as yet assessed the effect of cryopreservation on these vacuoles, although sperm freezing-thawing procedures are known to affect sperm quality. Examination of the vacuoles before and after freezing-thawing would indicate whether the same normality criteria can be applied for frozen as for fresh spermatozoa when performing intracytoplasmic morphologically selected sperm injection. In 27 sperm samples from fertile men, analysis of conventional sperm parameters (motility, vitality, percentage of normal forms) and a morphological analysis at high magnification (×6000) using image analysis software was performed before freezing and after thawing. Whereas there were expected decreases in motility (P<0.0001), vitality (P<0.001) and percentage of normal forms (P<0.05) after cryopreservation, there was no evidence for any difference in any vacuolar criteria (relative vacuole area, total vacuole area, vacuole area in the anterior, median and basal parts of the head, percentage of spermatozoa with a vacuole area ≤6.5% and percentage of spermatozoa with a vacuole area >13%). Freezing-thawing procedures have no effect on human sperm vacuoles.

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Cryosurvival of Human Spermatozoa Frozen in Eight Different Buffer Systems

Weidel

Prins

1987

Journal of Andrology

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Effects of various concentrations of glycerol on post-thaw motility and velocity of human spermatozoa

Cited by 12 publications

References 19 publications

Improved conception rates in sows inseminated with cryopreserved boar spermatozoa prepared with a more optimal combination of osmolality and glycerol in the freezing extender

Improved conception rates in sows inseminated with cryopreserved boar spermatozoa prepared with a more optimal combination of osmolality and glycerol in the freezing extender

Sperm vacuoles are not modified by freezing–thawing procedures

Cryosurvival of Human Spermatozoa Frozen in Eight Different Buffer Systems

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