Effects of vaporized perfluorohexane and partial liquid ventilation on regional distribution of alveolar damage in experimental lung injury

Spieth, Peter M.; Knels, Lilla; Kasper, Michael; Quelhas, André Domingues; Wiedemann, Bärbel; Lupp, Amelie; Hübler, Matthias; Neto, A. Giannella; Koch, Thea; Abreu, Marcelo Gama de

doi:10.1007/s00134-006-0479-9

Cited by 3 publications

(4 citation statements)

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“…The choice of perfluorocarbons was based on their insolubility in aqueous solutions, their nontoxicity, their inertness, and their precedent usage for biomedical applications. [18][19][20] The air bubbles or perfluorocarbon droplets (10 lL) were placed in contact with the cellular surface. The stage was tilted at a rate of 0.58/s, and the advancing and receding angle values were measured every second until the bubble/droplet rolled off the surface.…”

Section: Contact Angle/surface Energy and Hysteresismentioning

confidence: 99%

Effect of Stratification on Surface Properties of Corneal Epithelial Cells

Yáñez-Soto

Leonard

Raghunathan

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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Citation: Yáñez-Soto B, Leonard BC, Raghunathan VK, et al. Effect of stratification on the surface properties of corneal epithelial cells. Invest Ophthalmol Vis Sci. 2015;56:8340-8348. DOI:10.1167/iovs.15-17468 PURPOSE. The purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity.METHODS. hTCEpi cells were cultured to confluence in serum-free medium. The medium was then replaced by stratification medium to induce mucin biosynthesis. The mucin expression profile was analyzed using quantitative PCR and Western blotting. Contact angles were measured using a two-immiscible liquid method, and contact angle hysteresis was evaluated by tilting the apparatus and recording advancing and receding contact angles. The spatial distribution of mucins was evaluated with fluorescently labeled lectin. RESULTS. hTCEpi cells expressed the three main ocular mucins (MUC1, MUC4, and MUC16) with a maximum between days 1 and 3 of the stratification process. Upon stratification, cells caused a very significant increase in contact angle hysteresis, suggesting the development of spatially discrete and heterogeneously distributed surface features, defined by topography and/or chemical functionality. Although atomic force microscopy measurements showed no formation of appreciable topographic features on the surface of the cells, we observed a significant increase in surface chemical heterogeneity. CONCLUSIONS.The surface chemical heterogeneity of the corneal epithelium may influence the dynamic behavior of tear film by ''pinning'' the contact line between the cellular surface and aqueous tear film. Engineering the surface properties of corneal epithelium could potentially lead to novel treatments in dry eye disease.

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Section: Contact Angle/surface Energy and Hysteresismentioning

confidence: 99%

Effect of Stratification on Surface Properties of Corneal Epithelial Cells

Yáñez-Soto

Leonard

Raghunathan

et al. 2015

Invest. Ophthalmol. Vis. Sci.

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“…For acquisition of airway flow, as well as airway and esophageal pressures, a LabVIEw-based data acquisition system 17 (National Instruments, Austin, TX) was used, as described elsewhere. 18 Estimation of intra-abdominal pressure was obtained with a balloon pressure probe (Erich Jäger) filled with 5 ml of distillated water and placed between the bladder and the vesicouterine excavation after a mini-laparotomy, connected to a differential pressure transducer (BD DTXPlus™, Becton Dickinson, NJ), and zeroed at the mid-axillary line. A urinary catheter was inserted into the bladder through the mini-laparotomy.…”

Section: Instrumentation and Induction Of Alimentioning

confidence: 99%

Effects of Intravascular Volume Replacement on Lung and Kidney Function and Damage in Nonseptic Experimental Lung Injury

et al. 2013

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Background: Intravascular volume replacement is often required in the presence of increased pulmonary capillary leakage, for example in patients with volutrauma with major hemorrhage. In the present study, the effects of Ringer's acetate (RA), gelatin-polysuccinate (GEL), and a modern hydroxyethyl starch (HES, 6% 130/0.42) on lung and kidney function and damage were compared in a twohit model of acute lung injury. The authors hypothesized that GEL and HES, compared to RA: (1) reduced lung histological damage, (2) impaired kidney morphology and function. Methods: Acute lung injury was induced in 30 anesthetized pigs by tidal volumes approximately 40 ml/kg, after saline lung lavage. Protective ventilation was initiated and approximately≈25% of estimated blood volume was drawn. Animals were randomly assigned to receive RA, GEL, or HES (n = 10/group) aimed at approximately 90% of intrathoracic blood volume before blood drainage. Effects of Intravascular Crystalloid versus Colloids in Nonseptic Lung InjuryResults: Fluid volumes were higher with RA (2,250 ± 764 ml) than GEL (704 ± 159 ml) and HES (837 ± 82 ml) (P < 0.05). Compared to RA, HES reduced diffuse alveolar damage overall, and GEL in nondependent zones only. GEL and HES yielded lower wet-to-dry ratios compared to RA (6.5 ± 0.5 and 6.5 ± 0.6 vs. 7.9 ± 0.9, respectively, P < 0.05). HES and RA resulted in less kidney damage than GEL, but kidney function did not differ significantly among groups. Compared to GEL, HES yielded lower lung elastance (55 ± 12 vs. 45 ± 13 cm H 2 O/l, P < 0.05) and intra-abdominal pressure (15 ± 5 vs. 11 ± 4 cm H 2 O, P < 0.05).Conclusions: In this model of acute lung injury, intravascular volume expansion after major hemorrhage with HES yielded less lung damage than RA and less kidney damage than GEL.

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“…However, alternative forms of PFC application such as aerosol or vapor administration have shown promising effects without causing typical problems and shortcomings of liquid ventilation. In this context, vaporized PFH was associated with improved oxygenation and lung mechanics in experimental ARDS, protected animals from ventilator-induced lung injury, and improved lung histology in oleic acid-injured pigs to a far greater degree compared with liquid ventilation (3,8,13,45). Whether the anti-inflammatory effects of PFH shown by us in recent in vitro studies contribute to this organoprotection remains unclear (15,25).…”

Section: Discussionmentioning

confidence: 92%

“…These data and the fact that PFC per se do not augment in vitro bacterial growth suggest that nosocomial pneumonia is not increased by liquid ventilation with PFC (29,40). There are no animal trials using PFH in the setting of bacterial lung infection, and the experimental protocols of all studies performed in this field were too short to allow development of VAP (3,8,13,45). However, our in vitro results support the assumption that despite previously demonstrated anti-inflammatory effects (15,25), PFH does not seem to impair lung bactericidal defense, and therefore our initial hypothesis cannot be confirmed.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro

Haufe¹,

Koenigshausen²,

Knels³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

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Application of liquid, aerosolized, and vaporized perfluorocarbons (PFC) in acute lung injury has shown anti-inflammatory effects. Although this may be beneficial in states of pulmonary hyperinflammation, it also could increase susceptibility to nosocomial lung infection. We hypothesized that PFC impair cellular host defense and therefore investigated in an in vitro model the influence of perfluorohexane (PFH) on crucial mechanisms of bacterial elimination in human neutrophils and monocytes. Using scanning and transmission electron microscopy, we could show membrane-bound and ingested PFH particles that morphologically did not alter adherence and phagocytosis of Escherichia coli or leukocyte viability. The amount of adherent and phagocytosed bacteria as determined by flow cytometry was not influenced in cells only pretreated with PFH for 1 and 4 h. When PFH was present during E. coli challenge, bacterial adherence was decreased in polymorphonuclear neutrophils, but respective intracellular uptake was not impaired and was even significantly promoted in monocytes. Overall, E. coli-induced respiratory burst capacity was not reduced by PFH. Our findings provide evidence that key functions of innate host defense are not compromised by PFH treatment in vitro.phagocytosis; burst; perfluorohexane; anti-inflammatory; immune LIQUID VENTILATION WITH PERFLUOROCARBONS (PFC) has been successfully applied in experimental and human acute lung injury (18,20,27,36). Beside positive effects on gas exchange, lung mechanics, and ventilation/perfusion matching, animals treated with liquid ventilation also exhibited attenuated pulmonary damage with concomitant reduction in pulmonary neutrophil sequestration (19,21,32,39). A clinical trial investigating PFC treatment in trauma patients with acute lung injury demonstrated reduced neutrophil counts and decreased levels of proinflammatory cytokines in bronchoalveolar lavage fluid (7). Although removal of alveolar inflammatory exudate by dense, water-insoluble PFC probably contributes to mitigation of pulmonary hyperinflammation, a direct interference with immune cell function may also be involved. This proposition is supported by recent in vivo studies using aerosolized PFC in a surfactant-washout model that reported PFC to decrease mRNA expression of proinflammatory cytokines and adhesion molecules in lung tissue samples as well as in microdissected alveolar macrophages and pulmonary parenchymal cells (42,49,50). Further evidence for cellular anti-inflammatory effects of PFC is provided by in vitro experiments showing a decreased response of activated polymorphonuclear neutrophils (PMN) and monocytic cells in terms of cytokine secretion, chemotaxis, cyclooxygenase-2 (COX-2) expression, and NF-B activation (5,25,37,46,51). Liquid ventilation with PFC did not result in improved survival or reduced need for ventilatory support in recent multicenter trials of patients with acute respiratory distress syndrome (ARDS) (17, 24). Nevertheless, intrinsic anti-inflammatory properties of PFC might b...

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Effects of vaporized perfluorohexane and partial liquid ventilation on regional distribution of alveolar damage in experimental lung injury

Cited by 3 publications

References 0 publications

Effect of Stratification on Surface Properties of Corneal Epithelial Cells

Effect of Stratification on Surface Properties of Corneal Epithelial Cells

Effects of Intravascular Volume Replacement on Lung and Kidney Function and Damage in Nonseptic Experimental Lung Injury

Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro

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