Effects of Vacuum Packaging on NAC Gene Expression in Fresh-cut Lotus Root

Min, Ting; Niu, Lifang; Xie, Jun; Yang, Yi; Wang, Limei; Ai, Youwei; Wang, Hongxun

doi:10.21273/jashs04806-19

Cited by 11 publications

(7 citation statements)

References 27 publications

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“…In addition, NnANT and NnAP2 have been reported as potential negative regulators of lotus seed size and development ( Li et al, 2018b ). Moreover, the lotus NAC genes have been implicated in root browning under anaerobic stress and the formation of adventitious roots ( Cheng et al, 2019 ; Min et al, 2019 ). However, despite previous reports, comprehensive mapping of genome-wide NAC TF family, which could potentially improve our knowledge on the gene family function, distribution, and evolution in sacred lotus is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

Song

Liu

Gao³

et al. 2022

Front. Genet.

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NAC (NAM, ATAF, and CUC) is a ubiquitously expressed plant-specific transcription factor (TF) family which is involved in the regulation of various biological processes. However, a systematic characterization of NAC gene family is yet to be reported in lotus. Here, 82 NnNAC genes which included five predicted membrane-bound NAC proteins were identified in the lotus genome. Phylogenetic analysis revealed seven-subfamily clusters (I–VII) of NnNAC proteins, with homologous gene pairs displaying similar conserved motifs and gene structure characteristics. Transactivation assay of NnNAC proteins revealed an extensive transcriptional activation capacity which is mediated by the highly divergent C-terminal activation domain (AD). Expression analysis of NnNAC genes in lotus tissues showed high transcript levels in root, stamen, petal and seed coat. In addition, 30 and 29 differentially expressed NnNAC candidate genes putatively involved in lotus seed development and response to complete submergence stress, respectively, were identified. Overall, our study provides potentially useful candidate gene resources for future molecular breeding of lotus varieties with novel agronomic traits.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

Song

Liu

Gao³

et al. 2022

Front. Genet.

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“…Other research studies showed that phenylalanine ammonia lyase (PAL) and POD activities positively correlated with NAC expression. − Thus, the low level expression of mdm-miR164b after H 2 S treatment increased the expression of NAC and might improve the antioxidant activity of fresh-cut apples, which was probably related to browning inhibition. However, the case difference in the delaying browning of fresh-cut lotus root by vacuum packaging, NnNAC1/4 was significantly suppressed, while NnNAC2 got induced . This discrepancy may result from the species-specific NAC member, as the function of NAC may be related to its types and plant species.…”

Section: Discussionmentioning

confidence: 95%

“…However, the case difference in the delaying browning of fresh-cut lotus root by vacuum packaging, NnNAC1/4 was significantly suppressed, while NnNAC2 got induced. 39 This discrepancy may result from the species-specific NAC member, as the function of NAC may be related to its types and plant species.…”

Section: ■ Discussionmentioning

confidence: 99%

miRNA and Degradome Sequencing Identify miRNAs and Their Target Genes Involved in the Browning Inhibition of Fresh-Cut Apples by Hydrogen Sulfide

Chen

Liu

Jiang

et al. 2020

J. Agric. Food Chem.

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Surface browning is the major limit for the shelf life of fresh-cut apples, and hydrogen sulfide (H 2 S) treatment can effectively inhibit the browning. However, the molecular mechanism on how fresh-cut apples respond to H 2 S was poorly understood. MicroRNAs (miRNAs) are a class of endogenous small noncoding RNAs, which regulate multiple crucial biological processes in almost all aspects of the life cycle. Herein, 12 small RNA libraries and one mixed degradome library were constructed from control and H 2 S-treated fresh-cut apples immediately after treatment (C0 and S0) and 6 d of storage (C6 and S6) at 4 °C. The results identified nine (three upregulated and six downregulated) and 10 (two upregulated and eight downregulated) differentially expressed miRNAs (DEmiRNAs) in S0 versus C0 and S6 versus C6, respectively. The target genes of DEmiRNAs were transcription factors and functional proteins. The miR156 targeting SPL, miR164 targeting NAC, miR319 targeting TCP4, GAMYB, and acyl-CoAbinding protein 4, and miR6478 targeting patatin-like protein 2 might play important roles in the browning inhibition of fresh-cut apples by H 2 S via regulating the ROS, phenylpropanoid, and lipid metabolism. The results give valuable information for further studying the role of miRNA in regulating browning processes and the underlying molecular mechanism of H 2 S treatment on browning inhibition.

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“…Various methods have been used to prolong the storage‐time of fresh‐cut lotus root. Oxalic acid treatment or vacuum packaging was able to suppress lotus browning by alleviating the activity of POD, PPO, and phenylalanine ammonia lyase (PAL) 6,7 . Enhancement of antioxidant enzyme activity has also suppressed the browning of fresh‐cut foods by reducing the levels of reactive oxygen species (ROS) 8 …”

Section: Introductionmentioning

confidence: 99%

Melatonin‐induced myeloblastosis viral oncogene homologs alleviate fresh‐cut lotus root browning during storage by attenuating flavonoid biosynthesis and reactive oxygen species

et al. 2023

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BACKGROUND Lotus roots (Nelumbo nucifera Gaertn.) are rich in nutrients and have ornamental and food value. However, browning has caused huge economic losses and security risks during the storage and harvesting of fresh‐cut lotus. This study investigated the role of melatonin in inhibiting lotus browning, and illustrates its molecular mechanism. RESULTS The application of melatonin effectively retarded the process of lotus browning, enhanced reactive oxygen species (ROS) scavenging enzyme activity, and inhibited the activity of polyphenol oxidase (PPO), and peroxidase (POD). Melatonin reduced flavonoid content, and decreased enzymatic activity in flavonoid biosynthesis. Transcriptome Sequencing (RNA‐seq) was used to screen the genes regulated by exogenous melatonin when defending against fresh‐cut lotus browning. Gene co‐expression analysis (GCN) indicated that the transcription factors MYB5, MYB6, and MYB308, activated by melatonin, were negatively related to the expression of PPO and the genes related to flavonoid and phenylpropanoid biosynthesis. These myeloblastosis viral oncogene homologs (MYBs) were positively related to the expression of genes encoding the enzymes in glutathione metabolism. CONCLUSION Melatonin retarded lotus browning by transcriptional suppression of key genes associated with flavonoid and phenylpropanoid biosynthesis through the stimulation of MYB5, MYB6, and MYB308. © 2023 Society of Chemical Industry.

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Effects of Vacuum Packaging on NAC Gene Expression in Fresh-cut Lotus Root

Cited by 11 publications

References 27 publications

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

Genome-Wide Characterization and Comprehensive Analysis of NAC Transcription Factor Family in Nelumbo nucifera

miRNA and Degradome Sequencing Identify miRNAs and Their Target Genes Involved in the Browning Inhibition of Fresh-Cut Apples by Hydrogen Sulfide

Melatonin‐induced myeloblastosis viral oncogene homologs alleviate fresh‐cut lotus root browning during storage by attenuating flavonoid biosynthesis and reactive oxygen species

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