Effects of vacuum infiltration, Agrobacterium cell density and acetosyringone concentration on Agrobacterium-mediated transformation of bread wheat

Ashrafi‐Dehkordi, Elham; Alemzadeh, Abbas; Tanaka, Nobukazu; Razi, Hooman

doi:10.1007/s00003-020-01312-y

Cited by 4 publications

(3 citation statements)

References 36 publications

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“…Combining the optimum transient transformation conditions that were obtained, C. camphor leaves were infected with A. tumefaciens containing GCaMP3 with a bacterial Agrobacterium is not suitable for gene transformation during the lag phase due to its low activity. However, it has very high activity in the middle and later logarithmic phases and is still not suitable for gene transformation, as the bacterium produces more toxins to disrupt the plant's physiological functions and even cause cell death [58,59]. When C. camphora was infected with the infection solution (OD 600 of 0.7) separately prepared using A. tumefaciens GV3101 with OD 600 growth densities of 0.3, 0.5, 0.7, 0.9, and 1.1, the strongest fluorescence intensity was detected at OD 600 of 0.5 and 0.7 without significant differences between them (Figure 5B).…”

Section: Effects Of High Temperature On the Fluorescence Intensitymentioning

confidence: 99%

Establishment of a Transient Transformation Protocol in Cinnamomum camphora

Zhang,

Hu,

Yang

et al. 2023

Forests

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Cinnamomum camphora is an excellent evergreen broad-leaved tree species with strong stress tolerance, but its molecular character revelation as well as ecological and economic value improvement were limited due to the lack of a genetic transformation system. To establish a simple and efficient transient transformation system for uncovering the molecular mechanism of plant tolerating stresses and promoting the selective breeding of good varieties, the infection method, co-cultivation time, infection solution concentration, and growth density of Agrobacterium tumefaciens containing green fluorescent protein (GFP)-based calmodulin protein 3 gene (GCaMP3) were identified by monitoring the fluorescence emitted from GCaMP3 bound to Ca2+. Meanwhile, the transient transformation effects were evaluated via cytoplasmic Ca2+ concentration variations at high temperatures of 35 °C and 40 °C. When C. camphora leaves were infected with A. tumefaciens containing GCaMP3 via injection and soaking, no significant difference was detected in the fluorescence intensity over 48 h, indicating that the two infection methods had the same transient transformation efficiency. By prolonging the co-cultivation time, the fluorescence intensity gradually increased, reached its strongest at the 48th h, and then gradually declined. For the infection solution concentration, an OD600 of 0.7 led to the strongest fluorescence intensity, with an increase of 42.2%, 13.7%, 4.2%, and 14.2%, respectively, compared to that at OD600 of 0.5, 0.6, 0.8, and 0.9. When A. tumefaciens growth density OD600 was 0.5–0.7, the strongest fluorescence intensity was detected after transient transformation. Combining these optimum conditions, GCaMP3 was transferred into C. camphora, which indicated the variations in cytoplasmic Ca2+ concentration at high temperatures, with the fluorescence intensity at 35 °C and 40 °C increasing by 12.6% and 30.6%, respectively, in contrast to that at 28 °C. Therefore, it should be an efficient transient transformation system for C. camphora, with A. tumefaciens growth density OD600 of 0.5–0.7, infection solution concentration OD600 of 0.7, and co-cultivation time of 48 h by using both injection and soak infection methods, which is beneficial for uncovering the Ca2+ signal transduction in the plant tolerating stresses and promoting its molecular biology development and selective breeding of good varieties.

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Section: Effects Of High Temperature On the Fluorescence Intensitymentioning

confidence: 99%

Establishment of a Transient Transformation Protocol in Cinnamomum camphora

Zhang,

Hu,

Yang

et al. 2023

Forests

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“…This can be exploited to deliver any chimeric genes into the plant cells of choice using existing protocols aiming at the highest transformation efficiency. [129][130][131][132][133][134] Alternatively, the recombinant DNA/RNA can be directly delivered by the biolistic method, employing gold particles or tungsten covered with a linear expression cassette or an expression vector harbouring the chimeric gene. 135 The biolistic method causes multiple transgene integration, although lower concentrations (in nanograms) lead to an increased frequency of plants with single-copy.…”

Section: Preparation Of Transgenic Plantsmentioning

confidence: 99%

“…The former is based on the ability of Agrobacterium tumefaciens , a native soil pathogen, to randomly introduce its T‐DNA harbouring transformational construct into the nuclear genome of plants. This can be exploited to deliver any chimeric genes into the plant cells of choice using existing protocols aiming at the highest transformation efficiency 129–134 . Alternatively, the recombinant DNA/RNA can be directly delivered by the biolistic method, employing gold particles or tungsten covered with a linear expression cassette or an expression vector harbouring the chimeric gene 135 .…”

Section: Molecular Farming Of Fish Vaccinesmentioning

confidence: 99%

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

Mičúchová

Piačková

Frébort

et al. 2022

Reviews in Aquaculture

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Vaccines represent one of the most promising strategies for mitigating economic losses imposed by infectious diseases to global aquaculture. While the majority of commercial vaccines employ injection as the main route of delivery, a substantial body of research explores other avenues for the stimulation of protective immunity. Oral vaccines, representing the most favourable and convenient solution for the aquaculture industry, have been at the forefront of scientific interest for the last couple of decades. The orchestrated effort resulted in identifying the main roadblocks on the path to an ideal edible vaccine and provided several ingenious solutions improving the activation of immunity, ensuring the stability of administered antigens and their uptake upon the passage through the stomach. In the presented review, we describe advances in the available knowledge of the processes prerequisite for developing protective mucosal vaccines and focus readers' attention on the untapped potential of plant‐based production systems in this effort. We propose that these approaches not only meet production demands but also fulfil all requirements of an oral vaccine, addressing all the obstacles, previously solved via separate strategies, in one platform. Thus, combined with the available knowledge of molecular adjuvants and produced for a fraction of the cost, plant‐based production, so‐called molecular farming, is an ideal candidate for vaccine development, paving the road to sustainable aquaculture.

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Agrobacterium tumefaciens-Mediated Plant Transformation: A Review

Azizi-Dargahlou

Pouresmaeil

2023

Mol Biotechnol

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Effects of vacuum infiltration, Agrobacterium cell density and acetosyringone concentration on Agrobacterium-mediated transformation of bread wheat

Cited by 4 publications

References 36 publications

Establishment of a Transient Transformation Protocol in Cinnamomum camphora

Establishment of a Transient Transformation Protocol in Cinnamomum camphora

Molecular farming: Expanding the field of edible vaccines for sustainable fish aquaculture

Agrobacterium tumefaciens-Mediated Plant Transformation: A Review

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