Effects of UV wavelength on cell damages caused by UV irradiation in PC12 cells

Abstract

Ultraviolet (UV) radiations present in sunlight are a major etiologic factor for many skin diseases and induce DNA damage through formation of cyclobutane pyrimidine dimer (CPD). This study was conducted to determine the toxicological effects of different wavelengths (250, 270, 290, and 310 nm) and doses of UV radiation on cell viability, DNA structure, and DNA damage repair mechanisms in a PC12 cell system. For this, we evaluated cell viability and CPD formation. Cell survival rate was markedly decreased 24 h after UV irradiation in a dose-dependent manner at all wavelengths (except at 310 nm). Cell viability increased with increasing wavelength in the following order: 250 < 270 < 290 < 310 nm. UV radiation at 250 nm showed the highest cell killing ability, with a median lethal dose (LD50) of 120 mJ/cm2. The LD50 gradually increased with increase in wavelength. Among the 4 wavelengths tested, the highest LD50 (6000 mJ/cm2) was obtained for 310 nm. CPD formation decreased substantially with increasing wavelength. Among the 4 wavelengths, the proportion of CPD formation was highest at 250 nm and lowest at 310 nm. On the basis of LD50 values for each wavelength, PC12 cells irradiated with UV radiation of 290 nm showed maximum DNA repair ability, whereas those irradiated with the 310-nm radiation did not show any repair ability. Toxicity of UV radiation varied with wavelengths and exposure doses

One‐Step Photoactivation of a Dual‐Functionalized Bioink as Cell Carrier and Cartilage‐Binding Glue for Chondral Regeneration

“…[ 38 , 72 ] For example, UV-A and near UV wavelengths are known to generate reactive oxygen species and free radicals that can indirectly damage DNA. [ 73 , 74 ] In this context, hydrogels with gelation chemistries based on radical initiation have been shown to consume such potentially harmful radicals, thus protecting the embedded cells, and allowing to identify safer crosslinking windows. [ 38 , 75 ] However, in a clinical setting, it can be challenging to confine such photo-crosslinking reactions exclusively to the defect volume.…”

Fundamentals of light-cell–polymer interactions in photo-cross-linking based bioprinting

“…have investigated the toxicological effects of a range of UV wavelengths (250 nm–310 nm). 72 The energy doses leading to cell death increased by increasing the wavelength. The lethal dose for killing cells at 250 nm was 120 mJ/cm 2 , while the doses required to damage cells working at 310 nm was 6 J/cm 2 .…”

Wavelength-normalized spectroscopic analysis of Staphylococcus aureus and Pseudomonas aeruginosa growth rates

“…We characterized the bacteria over a wavelength range of 350 nm through 800 nm. At wavelengths as short as 250 nm, we would expect to see damage to the cell [31]. Therefore, we did not go shorter than 350 nm, so as to avoid cellular damage.…”