Effects of uridine on the growth and differentiation of HL-60 leukemia cells

ASB-2 Inhibits Growth and Promotes Commitment in Myeloid Leukemia Cells

“…This observation is in agreement with the fact that, in PML-RAR␣-expressing cells exposed to the differentiative stimulus of RA, G 1 arrest occurs as a late phase not causally linked to commitment and differentiation (13). It should also be noticed that, in uridine-induced HL-60 cells, an arrest in the G 1 phase is not required for differentiation as this process is preceded by accumulation of cells in the G 2 /M phase of the cell cycle (47).…”

Nucleoside anticancer drugs: the role of nucleoside transporters in resistance to cancer chemotherapy

“…A variety of mechanisms are thought to contribute to the regulation of NTs in different cell types. When HL-60 cells were induced to differentiate using chemical inducing agents, changes in es and ei transport activities, now known to be mediated by the hENT1 and hENT2 proteins, were observed (Chen et al, 1986;Delicado et al, 1991;Lee et al, 1991;Sokoloski et al, 1991;Lee, 1994). NBMPR-binding sites decreased while the dissociation constant (K d ) remained unchanged and hENT1 transport activity decreased upon induction of differentiation in HL-60 cells (Chen et al, 1986).…”

Uridine induces differentiation in human neuroblastoma cells via Protein Kinase C epsilon

“…As reported in the literature, a new type of synthetic bicyclic pyrimidine compounds promotes neurite outgrowth in human neuroblastoma cells and enhances the neurotrophic effects of the basic fibroblast growth factor (Awaya et al, 1993;Sanjo et al, 1998). Moreover, exogenous uridine induced a decrease in cell proliferation in HL-60 cells, resulting in the accumulation of cells in the G 2 /M phase (Sokoloski et al, 1991). As we have illustrated in Figure 1, exogenous uridine lowered cell number dose-dependently in LAN-5 cells.…”

“…In our experiments, LAN-5 neuroblastoma cells activate cell differentiation after treatment with uridine or they activate cellular death after treatment with adenosine (data not shown). These effects were both described in other tumor cells as HL-60 cells (Sokoloski et al, 1991;Kim et al, 1998).…”

“…This uridine-induced decrease was inhibited by dipyridamole, an inhibitor of nucleotides and nucleosides uptake. We used a micromolar concentration of uridine instead of the millimolar concentration used by Sokoloski et al(1991), the latter having proved to be toxic in our experiment with LAN-5 cells (data not shown). Recently, the importance of uridine 5Ј-triphosphate (UTP) in the regulation of cell proliferation has been reported (Whitmarsh and Davis, 2000).…”