Effects of unsaturated fatty acids on cell membrane functions

Hagve, Tor‐Arne

doi:10.3109/00365518809085746

Cited by 47 publications

(12 citation statements)

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“…It has been observed in mammals that the variation of the fatty acid composition in the diet produce changes in the fatty acid profile of the tissue affecting the adipocyte metabolism. The changes in the adipocyte metabolism can affect the efficiency of substrate accumulation (Su and Jones, 1993), the permeability of the cell membrane, the ability to recognize extracellular molecules like hormones (Hagve, 1988) and the hyperplasia of the tissue (Launy et al, 1972;Soriguer Escofet et al, 1996). Therefore, this lack of response to the treatments observed in the lipolytic levels and gene expression in adipocytes from animals fed wih the VO diet might be associated to the changes in the fatty acid profile of the tissue.…”

Section: Discussionmentioning

confidence: 99%

Changes in adipocyte cell size, gene expression of lipid metabolism markers, and lipolytic responses induced by dietary fish oil replacement in gilthead sea bream (Sparus aurata L.)

Cruz-García

Sánchez-Gurmaches

Bouraoui

et al. 2011

Comparative Biochemistry and Physiology Part A: Molecular &

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The effects of fish oil (FO) substitution by 66% vegetable oils in a diet with already 75% vegetable protein (66VO) on adipose tissue lipid metabolism of gilthead sea bream were analysed after a 14-month feeding trial. In the last 3 months of the experiment, a FO diet was administrated to a 66VO group (group 66VO/FO) as a finishing diet. Hormone-sensitive lipase (HSL) activity was measured in adipose tissue and adipocyte size, and HSL, lipoprotein lipase and liver X receptor gene expression in isolated adipocytes, on which lipolysis and glucose uptake experiments were also performed. Lipolysis was measured after incubation with tumour necrosis factor-α (TNFα), linoleic acid, and two conjugated linoleic acid isomers. Glucose uptake was analysed after TNFα or insulin administration. Our results show that FO replacement increased lipolytic activity and adipocyte cell size. The higher proportion of large cells observed in the 66VO group could be involved in their observed lower response to fatty acid treatments and lower insulin sensitivity. The 66VO/FO group showed a moderate return to the FO conditions. Therefore, FO replacement can affect the morphology and metabolism of gilthead sea bream adipocytes which could potentially affect other organs such as the liver.

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Section: Discussionmentioning

confidence: 99%

Changes in adipocyte cell size, gene expression of lipid metabolism markers, and lipolytic responses induced by dietary fish oil replacement in gilthead sea bream (Sparus aurata L.)

Cruz-García

Sánchez-Gurmaches

Bouraoui

et al. 2011

Comparative Biochemistry and Physiology Part A: Molecular &

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“…The changes in lipid composition induced by the lipopeptide were probably consequences of membrane permeabilization due to the perturbing action of lipopeptides. In addition, the unsaturated degree (Geiser et al, 1994) of cellular fatty acids treated with the 30 μg/mL and 60 μg/mL lipopeptides was decreased from 29.3% to 28.3% and 23.4%, respectively, which indicated that the physiologic properties of membranes, such as fluidity and cellular functions, may be changed (Hagve, 1988). Table 1).…”

Section: Lipopeptide Induced Changes In Lipid Composition Of Cell Memmentioning

confidence: 98%

Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Tao

Zou

et al. 2010

Protein Cell

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Microbial lipopeptides play an important role in apoptosis induction of tumor cells. However, there is little knowledge about the relationship between apoptosis induction and membrane fatty acids. The present study focused on the effects of lipopeptides produced by Bacillus subtilis HSO121 on Bcap-37 cell lines. 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl (MTT) colorimetric assay and surface tension measurements, showed that the critical micelle concentration (CMC) was a critical level for the inhibitory activity of lipopeptides on the growth of Bcap-37 cells. Under the CMC, the order of least to greatest cytotoxicity effect on cancer cell lines by lipopeptides is C 13 -lipopeptide < C 14 -lipopepitde < C 15 -lipopeptide. Above CMC, all lipopeptides directly exert cytolytic activity. The flow cytometric analysis and Hoechst33258 staining experiments confirmed the apoptosis of Bcap-37 cell lines induced by lipopeptides in a dose-dependent manner. This apoptosis was associated with a significant decrease of the unsaturated degree of the cellular fatty acids of Bcap-37 cell lines due to the changes in the cellular fatty acids composition induced by the lipopeptide treatment. These results indicated that disturbance of the cellular fatty acid composition of breast cancer cell lines were related to in the cell apoptosis. Furthermore, significant difference in IC 50 values of tumor cells and normal cell showed that the lipopeptide exerted selective cytotoxicity on the cancer cells. Thus HSO121 lipopeptides may have potential applications as an anticancer leads.

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“…Furthermore the properties of membrane function such as fluidity, permeability and anchoring of membrane proteins depend considerably on membrane phospholipid fatty acid composition (Stubbs & Smith, 1984;Hagve, 1988). The control and regulation of membrane phospholipid composition is not clearly understood; however, it is well known that the dietary fatty acid profile markedly influences the incorporation of fatty acids into the membrane (Pan & Storlien, 1993;Ayre & Hulbert, 1996) and that regular physical activity per se is also a moderator of membrane phospholipid fatty acid composition in rats Helge et al 1999;Gorski et al 1999).…”

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confidence: 99%

Eccentric Contractions Affect Muscle Membrane Phospholipid Fatty Acid Composition in Rats

Helge

Therkildsen

Jørgensen

et al. 2001

Experimental Physiology

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This study investigated if prior eccentric contractions, and thus mechanical strain and muscle damage, exert an effect on the muscle membrane phospholipid fatty acid composition in rats, and whether a possible effect could be attenuated by dietary supplements. Twenty-three rats were randomised to three groups who received chow with added fish oil (n = 8), vitamin C (n = 8) or no supplement (n = 7). After 3 weeks of feeding, calf muscles on one side were stimulated electrically during anaesthesia causing eccentric contractions. Two days later the white gastrocnemius, a part of the stimulated calf muscle, was excised from both legs. In the muscles stimulated to contract eccentrically, compared to the control muscles, the proportion of arachidonic acid, C20:4,n_6 (17.7 ± 0.6; 16.4 ± 0.4 % of total fatty acids, respectively) and docosapentanoeic acid, C22:5,n_3 (2.9 ± 0.1 and 2.7 ± 0.1 % of total fatty acids, respectively) was uniformly higher across groups (P < 0.02) with no differences between diet groups. The proportion of long chain polyunsaturates was also significantly higher in the eccentrically contracted (39.9 ± 0.6 % of total fatty acids) compared to the control leg (38.2 ± 0.6 % of total fatty acids; P < 0.01). In contrast no differences were observed in the fatty acid composition of the triacylglycerols stored within the muscle. Thus one severe bout of eccentric contractions modulates the fatty acid composition of the muscle membrane phospholipids when compared to a control leg, and supplemental intake of fish oil or vitamin C did not attenuate this effect. Experimental Physiology (2001) 86.5, 599-604. 2196

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Effects of unsaturated fatty acids on cell membrane functions

Cited by 47 publications

References 0 publications

Changes in adipocyte cell size, gene expression of lipid metabolism markers, and lipolytic responses induced by dietary fish oil replacement in gilthead sea bream (Sparus aurata L.)

Changes in adipocyte cell size, gene expression of lipid metabolism markers, and lipolytic responses induced by dietary fish oil replacement in gilthead sea bream (Sparus aurata L.)

Effect of themicrobial lipopeptide on tumor cell lines: apoptosis induced by disturbing the fatty acid composition of cell membrane

Eccentric Contractions Affect Muscle Membrane Phospholipid Fatty Acid Composition in Rats

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