Effects of unilateral and bilateral labyrinthectomy on rat postural muscle properties: the soleus

Kasri, M.; Picquet, Florence; Falempin, Maurice

doi:10.1016/j.expneurol.2003.09.006

Cited by 21 publications

(17 citation statements)

References 59 publications

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“…Immediately after a bilateral labyrinthectomy, the soleus postural activity has been reported to decrease but to return to control level after a short period. However, after 17 days, a hyperactivity of the soleus muscle has been observed (Kasri et al, 2004). In our study, using Morey's model (hindlimb unloading), the gravitational load was maintained and thus, labyrinthine inputs remained persistent.…”

Section: Immediately After Humentioning

confidence: 79%

Physiologically adaptive changes of the L5 afferent neurogram and of the rat soleus EMG activity during 14 days of hindlimb unloading and recovery

De-Doncker¹,

Kasri²,

Picquet³

et al. 2005

Journal of Experimental Biology

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Section: Immediately After Humentioning

confidence: 79%

Physiologically adaptive changes of the L5 afferent neurogram and of the rat soleus EMG activity during 14 days of hindlimb unloading and recovery

De-Doncker¹,

Kasri²,

Picquet³

et al. 2005

Journal of Experimental Biology

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“…It can be considered as a synthesis of best existing techniques to induce labyrinthectomy in rodents (1,3,4,7,10,11,13,14,18,(23)(24)(25) with a novel standardized microsurgical approach to the mastoid bulla, and to the cochlea. Drilling into the cochlea with subsequent suctioning of the fluid and of cellular contents, as well as the ethanol washes, ensure the destruction of the Corti organ and of the stria vascularis.…”

Section: Discussionmentioning

confidence: 99%

“…By carefully widening it, the typical scalar structure of the cochlea can be recognized. Then, the cochlear cavity is washed with saline and its contents aspired repeatedly as suggested by several authors (1,3,4,10,13,14,24,25). Typical cellular debris of the Corti organ will appear in the suction fluid.…”

Section: Methodsmentioning

confidence: 99%

“…Most surgical techniques reported until now include extensive extirpation of the wall of the meatus acusticus externus and structures of middle ear (1,4,7,9,12,15,16,22,25). The specific anatomy of the rodent skull, however, provides an alternative surgical approach through the tympanic bulla (6,10,11,13). No publication, however, is available, with the exception of Hitier et al (11) with a sufficiently detailed description on how to expose the rat vestibular cavity surgically, while inducing minimum damage to the surrounding sensitive neck, temporal, and skull structures.…”

Section: Introductionmentioning

confidence: 99%

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A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

Nádasy

Raffai

Fehér

et al. 2016

Physiology International

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Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

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“…In a number of ground-based animal studies, using surgical bilateral labyrinthectomy in neonatal ferrets or adult rats, significant changes in the slow-type muscle soleus were observed [10,21]. Other reports confirmed that vestibulosympathetic reflex activity exerts a strong influence on the vegetative system with regard to maintenance of postural blood pressure control [15,26].…”

Section: Introductionmentioning

confidence: 99%

Increased myofiber remodelling and NFATc1-myonuclear translocation in rat postural skeletal muscle after experimental vestibular deafferentation

Luxa

Salanova

Schiffl

et al. 2013

VES

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Background: The vestibular system undergoes considerable modification during spaceflight [5]. This is paralleled by microgravity-induced muscle atrophy [6]. However, the possibility of vestibulo-autonomic regulatory mechanisms affecting skeletal muscle structure and function have not yet been addressed. Objective: We hypothesise that the vestibular system affects anti-gravitational skeletal muscle phenotype composition, size and the transcriptional factor called nuclear factor of activated T cells (NFATc1). Methods: In a laboratory study, we examined the morphological and histochemical properties including intramyocellular NFATc1 changes in slow-type soleus muscle of chemically labyrinthectomized rats (VLx; n=8) compared to a control group (Sham; n=6) after a period of one month. Results and conclusion: Neurochemical vestibular deafferentation resulted in smaller myofibre sizes, altered myofibre phenotype composition, high yields of hybrid fibre formation, and reduced myonuclear NFATc1 accumulation as signs of slow-type myofibre atrophy, myofibre type remodelling, and altered nuclear transcriptional activity in the postural soleus muscle of rats. We propose that vestibulo-autonomic modification of skeletal muscles occurs during prolonged microgravity. Our findings are likely to have implications for vestibular rehabilitation in clinical settings.

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Effects of unilateral and bilateral labyrinthectomy on rat postural muscle properties: the soleus

Cited by 21 publications

References 59 publications

Physiologically adaptive changes of the L5 afferent neurogram and of the rat soleus EMG activity during 14 days of hindlimb unloading and recovery

Physiologically adaptive changes of the L5 afferent neurogram and of the rat soleus EMG activity during 14 days of hindlimb unloading and recovery

A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process

Increased myofiber remodelling and NFATc1-myonuclear translocation in rat postural skeletal muscle after experimental vestibular deafferentation

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