Effects of Ultraviolet Radiation on Amphibians: Field Experiments

Blaustein, Andrew R.; Kiesecker, Joseph M.; Chivers, Douglas P.; Hokit, D. Grant; Marco, Adolfo; Belden, Lisa K.; Hatch, Audrey C.

doi:10.1093/icb/38.6.799

Cited by 154 publications

(150 citation statements)

References 38 publications

Supporting

Mentioning

147

Contrasting

Unclassified

Order By: Relevance

“…UV radiation has been proposed as a major stress factor for some amphibian populations (4,16,19). Moreover, it is important to consider that UV radiation may alter the chemical structure of some xenobiotics and thereby enhance toxicity.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Mitogen-activated protein kinase activation by oxidative and bacterial stress in an amphibian cell culture model.

Carter

Tabor

Bonner

et al. 2002

Environ Health Perspect

View full text Add to dashboard Cite

The decline of many amphibian species could be caused by their susceptibility to environmental pollutants that cause cellular stress and cell death. A variety of intracellular signal transduction pathways are activated by environmental stress factors, which result in cell death. Mitogen-activated protein kinases are intracellular signaling molecules that include the extracellular signal-regulated kinases (ERK-1 and ERK-2). We used cultured (italic)Xenopus(/italic) tadpole cells (XTC-2 cells) to investigate the activation of ERK by oxidative or bacterial stress, two environmental factors that could contribute to pollution in aquatic systems. We exposed XTC-2 cell monolayers to hydrogen peroxide or bacterial lipopolysaccharide and measured ERK activation by Western blotting using antibodies raised against phosphorylated ERK-1 and ERK-2. Only ERK-2 was detected in XTC-2 cells. Both hydrogen peroxide and lipopolysaccharide caused ERK-2 phosphorylation in a time- and concentration-dependent manner. Hydrogen peroxide caused a 20- to 30-fold increase in ERK-2 activation that peaked 30 min after treatment, and lipopolysaccharide induced a 5- to 10-fold increase in ERK-2 activation that peaked 60 min after treatment. PD98059, an inhibitor of the ERK pathway, reduced the cytotoxic response of XTC-2 cells to hydrogen peroxide or lipopolysaccharide. These data suggest that ERK-2 is an intracellular target of oxidative and bacterial stress in amphibians that mediates, at least in part, the cytotoxic response to hydrogen peroxide or lipopolysaccharide. Moreover, the (italic)Xenopus(/italic) (XTC-2) cell culture system could serve as a useful model to identify agents that might threaten amphibian populations and human health.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Transition metals and UV radiation cause MAP kinase activation via the generation of reactive oxygen species (14,15). In particular, UV radiation has been implicated as a major factor in amphibian mortality and deformities (16)(17)(18)(19).…”

mentioning

confidence: 99%

Mitogen-activated protein kinase activation by oxidative and bacterial stress in an amphibian cell culture model.

Carter

Tabor

Bonner

et al. 2002

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…This study suggests that amphibian pheromonal systems could be one of the systems mediating subtle effects of environmental chemicals. In addition to local climate change, habitat fragmentation, infectious disease, and increased UV-B irradiation, increased use of environmental chemicals has been suggested to be one of the major factors underlying recent amphibian declines (1)(2)(3)(35)(36)(37)(38)(39). Therefore, disruption of amphibian pheromonal systems by such chemicals are possibly one other mechanism facilitating population declines because the system plays a critical role in the daily life of amphibians such as conspecific recognition, migration, social behavior, and reproduction.…”

Section: Discussionmentioning

confidence: 99%

Endosulfan Exposure Disrupts Pheromonal Systems in the Red-Spotted Newt: A Mechanism for Subtle Effects of Environmental Chemicals

Park¹,

Hempleman²,

Propper³

2001

Environmental Health Perspectives

View full text Add to dashboard Cite

“…No amphibian studies on the effects of environmental stress on quantitative genetic parameters have yet been published, although the early aquatic developmental stages of amphibians are known to be extremely sensitive to various environmental stresses, such as ultraviolet-B radiation (Blaustein et al, 1998), chemical pollutants (Hecnar, 1995;Rosenshield et al, 1999;Bridges and Semlitsch, 2000) and acidification (Bö hmer and Rahman, 1990). In fact, the recent global decline of amphibian populations (Alford and Richards, 1999;Houlahan et al, 2000) is believed to be -at least in part -a reflection of worldwide increase in the levels of these stresses (Alford and Richards, 1999;Corn, 2000).…”

Section: Introductionmentioning

confidence: 99%

Genetic and maternal effect influences on viability of common frog tadpoles under different environmental conditions

2003

View full text Add to dashboard Cite

Effects of Ultraviolet Radiation on Amphibians: Field Experiments

Cited by 154 publications

References 38 publications

Mitogen-activated protein kinase activation by oxidative and bacterial stress in an amphibian cell culture model.

Mitogen-activated protein kinase activation by oxidative and bacterial stress in an amphibian cell culture model.

Endosulfan Exposure Disrupts Pheromonal Systems in the Red-Spotted Newt: A Mechanism for Subtle Effects of Environmental Chemicals

Genetic and maternal effect influences on viability of common frog tadpoles under different environmental conditions

Contact Info

Product

Resources

About