Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var. Li.

Wang, Yinping; Wang, Cuina; Guo, Mingruo

doi:10.3390/molecules24050939

Cited by 31 publications

(19 citation statements)

References 53 publications

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“…Anyway, both extracts (OP%US and 50% US) give evidence that the combined treatment improves the polysaccharide extraction by leveraging ultra-sonic treatment. Indeed, sonication destroys the plant cell walls and reduces the particle size which enhances the polysaccharides extraction [ 27 ]. Nevertheless, excessive US treatment induces chain cleavage and destruction of polymer chains due to the cavitation effects [ 28 ].…”

Section: Resultsmentioning

confidence: 99%

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon

et al. 2021

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The present study aims to investigate the properties of biopolymers extracted from a Lebanese onion non edible plant. The extraction was performed under mild conditions by varying the percentage of ultra-sound (US) treatment duration to a total extraction time of 30 min (0, 50, 100% US). The extracts were characterized using FTIR, SEC, GC-MS, TGA, and DSC analyses. The composition of the extracts was determined from the total carbohydrate content and protein content measurements. The thermal analyses indicate that all samples have high thermal stability. The antioxidant activities of the extracts were investigated, using β-carotene bleaching, scavenging activity of ABTS, metal chelating ability, and total antioxidant activity tests. The results indicate that the 50% US treatment leads to the best antioxidant activity. Biocompatibility of the extracts was evaluated using hemolysis and cytotoxicity assays. The results showed that 0 and 50% US samples are not toxic to human cells, in contrary to 100% US.

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Section: Resultsmentioning

confidence: 99%

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon

et al. 2021

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“…The significant differences in the structures can be attributed to the impact of acoustic cavitation and increased treatment time which jointly damaged the polysaccharides cell wall thus effecting structural changes. Wang, Wang, and Guo, () alluded to significantly modified structures with ultrasound treatment as compared to non‐ultrasound treatment.…”

Section: Resultsmentioning

confidence: 99%

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Chikari

Han

Wang

et al. 2020

J Food Process Engineering

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The study investigated the effect of dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and desalination on the yield, microstructure and antioxidant properties of Astragalus polysaccharides (APS). Extracts were desalinated for 10 (APS10), 20 (APS20), and 30 (APS30) minutes. A 28.40% experimental yield, well correlated to the response surface methodology predicted model was achieved. Thermodynamic parameters were non‐spontaneous, irreversible and endothermic. Moreover, ultrasound enhanced the desalination rate and did not alter the chemical structure in the APS. Fourier Transform Infrared Spectroscopy showed a similar spectrum in all the APS samples. High‐performance gel chromatography and scanning electron microscopy, respectively presented reduced molecular weights and altered microstructures in the samples. Monosaccharide analysis revealed the existence of xylose, mannose, galactose, glucose, arabinose, rhamnose, and ribose in the APS samples. Moreover, the APS20 showed strong hydroxyl and 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activities, thus showing its potency in reducing oxidative stress. Hence, this study demonstrates that coupled ultrasound and alcohol/salt ATPS is a sustainable technique, which generates high yields of simultaneously extracted products. Practical applications Astragalus root has been used as an effective complementary treatment to conventional medicine owing to its rich nutraceutical composition and its numerous health attributes. Commercialization of Astragalus supplements into functional foods and nutraceuticals requires continuous exploration of novel, low cost and reproducible methods that retain their bioactivity. In this work, a synergized and bio‐compatible dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase system was used to extract and purify Astragalus polysaccharides. Application of this technique in the nutraceutical industry reduces treatment time, lowers bulk energy consumption hence reducing energy costs. It promotes the use of low‐cost, non‐hazardous bio‐based solvents in treatment processes thus considerably reducing extraction costs and environmental degradation. Findings from this study exhibit the practicability of this technique in industrial application. It equips biotechnologists with information on improved, sustainable and safer ways of extracting and enriching the quality of nutraceutical compounds.

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“…FT-IR spectra of LBPs-5 and LBPs-6 were analyzed using a FT-IR spectrophotometer (spectral resolution of 1 cm −1 , scanned for 32 times) in the range of 400–4000 cm −1 . Approximately 1 mg samples were mixed with 100 mg dried KBr powder and then pressed into pellets for the FT-IR measurement [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina

Guo

Dai

et al. 2021

BMC Chemistry

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Background Fungal polysaccharides belong to a very important class of biological macromolecules in nature, and have complex monosaccharide composition and structure. These studies on structure and biological activity of fungal polysaccharides have become one of the research hotspots of scholars at home and abroad. Results This study was performed in order to understand the structural characteristics and antioxidant activity of polysaccharides from Lenzites betulina (LBPs). The LBPs were deproteinized using sevag method, and further purified by DEAE cellulose-52 column and Sephadex G-100 column chromatographies, then the two refined polysaccharides were obtained and named LBPs-5 and LBPs-6. Fourier transform infrared spectrometry (FT-IR) showed that LBPs-5 and LBPs-6 are typical β-pyranose with characteristic peaks of polysaccharides. The molecular weight of the two water-soluble polysaccharides were estimated to be 3.235 × 103 Da and 6.196 × 103 Da by HPGPC, respectively. HPLC with PMP derivatization analysis indicated that the monosaccharide compositions of LBPs-5 were mannose, glucuronic acid, glucose, and galactose in a molar ratio of 0.05:0.15:0.76:0.04. The monosaccharide compositions of LBPs-6 were mannose, glucuronic acid, and glucose, in a molar ratio of 0.04:0.17:0.79. Furthermore, the two water-soluble polysaccharides demonstrated strong scavenging effects on DPPH·, ABTS·+, ·OH and weak total reducing power, especially LBPs-6 was significantly stronger in scavenging rate than that of LBPs-5. Conclusions The outcome of the study indicated that LBPs had good potential as medicine and food.

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Effects of Ultrasound Treatment on Extraction and Rheological Properties of Polysaccharides from Auricularia Cornea var. Li.

Cited by 31 publications

References 53 publications

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon

Antioxidant Activity and Biocompatibility of Fructo-Polysaccharides Extracted from a Wild Species of Ornithogalum from Lebanon

Dual‐frequency ultrasound‐assisted alcohol/salt aqueous two‐phase extraction and purification of Astragalus polysaccharides

Isolation, structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina

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