Effects of ultra-high pressure treatment on structure and bioactivity of polysaccharides from large leaf yellow tea

Chen, Hao; Huang, Yuzhe; Zhou, Cancan; Xu, Tenglong; Chen, Xinyang; Wu, Qianzhen; Zhang, Kunfeng; Li, Yong; Li, Daxiang; Chen, Yan

doi:10.1016/j.foodchem.2022.132862

Cited by 41 publications

(13 citation statements)

References 36 publications

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“…LYP-S3 is a new type of active acidic polysaccharide with lower molecular weight and higher uronic acid content, the main skeleton of which was proposed to be a novel straight chain with α-1,4-linked Gal p A with O-acetyl. The antioxidant and hepatoprotective activities of crude polysaccharides isolated from Large yellow tea have been previously investigated, but they did not characterize its structure of the glycosidic bond sequence. , However, the type of glycosidic linkage and its attachment mode play a critical role in the biological activity of polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

“…The antioxidant and hepatoprotective activities of crude polysaccharides isolated from Large yellow tea have been previously investigated, but they did not characterize its structure of the glycosidic bond sequence. 34,35 However, the type of glycosidic linkage and its attachment mode play a critical role in the biological activity of polysaccharides. Macrophage-mediated chronic inflammation plays an important role in many diseases, and various strategies have been used to prevent or block the inflammatory response.…”

Section: ■ Discussionmentioning

confidence: 99%

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Structural Characterization and Macrophage Polarization-Modulating Activity of a Novel Polysaccharide from Large Yellow Tea

Wang

et al. 2022

J. Agric. Food Chem.

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A novel homogeneous polysaccharide (LYP-S3) that promotes the M2 polarization of macrophages was obtained from large yellow tea by a bioactivity-guided sequential isolation procedure and activity evaluation in the present study. Structural characterization revealed that LYP-S3 has an average molecular weight of 28.6 kDa and is composed of rhamnose, arabinose, galactose, glucose, and galacturonic acid at the molar ratio of 8.08:11.66:11.77:3.96:58.02. The main backbone of LYP-S3 consists of →4)-α-d-GalpA-6-OMe-(1→, β-d-GalpA-(1→, →4)-β-d-Galp-(→1, and →β-d-Galp-(1→, and the branches are composed of α-l-Araf-(→1, →5)-α-l-Araf-(1→, →2,4)-β-l-Rhap-(1→, →2)-β-l-Rhap-(1→, and →4)-β-d-Glcp-(1→. An in vitro bioactivity evaluation assay showed that LYP-S3 remarkably reduced the expression of M1 macrophage markers and increased the expression of M2 macrophage markers. In addition, LYP-S3 inhibited adipocyte differentiation and adipogenesis in 3T3-L1 adipocytes and blocked macrophage migration toward 3T3-L1 adipocytes in the cocultures of bone-marrow-derived monocytes and 3T3-L1 adipocytes. Furthermore, LYP-S3 promoted the M2 polarization of macrophages in cocultures. These findings suggested that LYP-S3 has a potential function in preventing inflammation and obesity.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Structural Characterization and Macrophage Polarization-Modulating Activity of a Novel Polysaccharide from Large Yellow Tea

Wang

et al. 2022

J. Agric. Food Chem.

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“…The peak at 1416 cm −1 was attributed to the C-H deformation vibration [ 21 ]. The peaks at 800–1200 cm −1 represented the area of carbohydrates [ 22 ]. The singles at 1148 cm −1 and 1079 cm −1 were due to the stretch vibration of C-O-C linkages and the pyranoid ring [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization of a Pleurotus sajor-caju Polysaccharide and Its Neuroprotection Related to the Inhibition of Oxidative Stress

et al. 2022

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A novel polysaccharide PSP2-1 was isolated and purified from Pleurotus sajor-caju. The structural characterization data displayed that the molecular weight of PSP2-1 was 44.9 kDa, and PSP2-1 consisted of fucose, galactose, glucose, and mannose. The methylation results showed that the glycosidic bonds of PSP2-1 included T-Fuc, 1,6-Gal, T-Glc, 1,6-Glc, 1,3,6-Glc, 1,3-Man, 1,2,6-Man, and T-Man. Neuroprotective studies indicated that PSP2-1 significantly improved the cell viability of the H2O2-induced oxidatively damaged neuronal cell HT22, reduced the release of LDH, inhibited apoptosis and release of cytochrome c, and alleviated the decline of mitochondrial membrane potential and ROS accumulation. Furthermore, PSP2-1 decreased the phosphorylation levels of cleaved PARP and cleaved caspase-3, and increased the ratio of bcl-2/bax. Additionally, PSP2-1 could inhibit the phosphorylation of MAPK family members including JNK, p38, and Erk. Finally, animal experiments showed that PSP2-1 could improve the oxidative stress injury and the learning and memory ability of mice with aging induced by D-galactose. Our results confirmed that PSP2-1 significantly ameliorated the oxidative stress injury, inhibited the apoptosis in H2O2-induced neuronal cells via MAPK pathway, and also improved cognition in mice with aging induced by D-galactose. Our research gives the foundation for the functional food application of P. sajor-caju polysaccharides in the future.

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“…showed a 12.01% extraction yield when being processed at a solid‐to‐liquid ratio of 1:15 (w/v) and a pressure of 460 MPa for 17 min 120 . The polysaccharide content in yellow tea leaf extracts increased by 1.31, 128.28, and 19.86% when UPE was operated at 200, 400, and 600 MPa respectively at 25 °C for 5 min 121 …”

Section: Extraction Methods For Polysaccharidesmentioning

confidence: 99%

Recent trends in extraction, purification, and antioxidant activity evaluation of plant leaf‐extract polysaccharides

Ahmad

Chatha

Iqbal

et al. 2022

Biofuels Bioprod Bioref

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This review elaborates on current advances in the extraction, purification, and antioxidant activity of plant leaf-extract polysaccharides. Polysaccharides are widely used as important ingredients in the food, pharmaceutical, and cosmetic industries. Researchers have been investigating useful sources of natural polysaccharides and developing green and feasible extraction procedures for polysaccharides. This review examines different methods for extracting polysaccharides from leaves, and discusses their advantages and limitations. Purification techniques for plant leaf-based polysaccharides were also highlighted, together with their antioxidant effects. Among different extraction methods, pressurizedliquid extraction and enzyme-assisted extraction are considered to be better for large-scale extraction of polysaccharides from plant leaves. This review could contribute to the design of leaf waste processes at a commercial level for the sustainable recovery of polysaccharides.

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Effects of ultra-high pressure treatment on structure and bioactivity of polysaccharides from large leaf yellow tea

Cited by 41 publications

References 36 publications

Structural Characterization and Macrophage Polarization-Modulating Activity of a Novel Polysaccharide from Large Yellow Tea

Structural Characterization and Macrophage Polarization-Modulating Activity of a Novel Polysaccharide from Large Yellow Tea

Structural Characterization of a Pleurotus sajor-caju Polysaccharide and Its Neuroprotection Related to the Inhibition of Oxidative Stress

Recent trends in extraction, purification, and antioxidant activity evaluation of plant leaf‐extract polysaccharides

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