Effects of two host-associated probiotics Bacillus mojavensis B191 and Bacillus subtilis MRS11 on growth performance, intestinal morphology, expression of immune-related genes and disease resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus iniae

Büyükdeveci, Miray Etyemez; Cengizler, İbrahim; Balcázar, José Luís; Demirkale, İbrahim

doi:10.1016/j.dci.2022.104553

Cited by 16 publications

(7 citation statements)

References 72 publications

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“…There are several studies that have reported the improvement of growth performances in Tilapia after B. subtilis feeding of in Nile tilapia. For example, Nile tilapia fed a basal diet supplemented with B. subtilis MRS11 at 1 × 10 8 CFU/g of feed for 60 days improved growth performances, intestinal morphology, immunity, and the survival rate after challenge with Streptococcus iniae ( 35 ). The dietary supplementation of mixed Bacillus strains (Sanolife ® PRO-F) to Nile tilapia, O. niloticus at 0.5–1 g/kg diet improved the growth, feed utilization, antioxidant property and immune parameters ( 36 ).…”

Section: Discussionmentioning

confidence: 99%

Influences of Bacillus subtilis and fructooligosaccharide on growth performances, immune responses, and disease resistance of Nile tilapia, Oreochromis niloticus

et al. 2023

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The present study investigated the effects of Bacillus subtilis and fructooligosaccharide (FOS) on growth performances, immunity improvement, and disease resistance of Nile tilapia (Oreochromis niloticus). The fish (24.5 ± 1.6 g) were fed a basal diet (G1), diets supplemented with 1 g/kg (G2), 3 g/kg (G3) and 5 g/kg (G4) of FOS as well as diets supplemented with 1 × 109 CFU/g (G5), 3 × 109 CFU/g (G6) and 5 × 109 CFU/g (G7) of B. subtilis for 56 days. After the feeding trial, the complement C3, IL-1β, TNF-α, IFN-γ, hsp70 gene expression in the liver was then analyzed by a quantitative Real-time PCR. Then, fish were infected with Streptococcus agalactiae, and the survival rate was recorded. The results showed that FOS and B. subtilis had no significant effect (P > 0.05) on growth performances and survival rate. Lysozyme activity was significantly greater in the G4, G5, G6, and G7 groups. Also, all fish fed FOS and B. subtilis showed significantly (P < 0.05) higher respiratory burst activity than other groups. The expressions of complement C3, IL-1β, TNF-α, IFN-γ, and hsp-70 in the liver were significantly higher for fish fed 5 g/kg of FOS as well as for fish that received any concentration level of B. subtilis (P < 0.05) used in the study. After the S. agalactiae challenge test, the survival rate of fish-fed diets supplemented with FOS and B. subtilis was slightly higher than for the control group. The results indicated that FOS and B. subtilis could stimulate immune responses and immune-related genes in tilapia. However, further investigation of other prebiotics or herbs in combination with B. subtilis is encouraged at molecular levels and screening for beneficial metabolites that may increasingly improve digestive enzymes, growth performances, and health benefits in tilapia. In addition, on-farm experiments are needed.

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Section: Discussionmentioning

confidence: 99%

Influences of Bacillus subtilis and fructooligosaccharide on growth performances, immune responses, and disease resistance of Nile tilapia, Oreochromis niloticus

et al. 2023

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“…Bacillus subtilis is one of the main sources of probiotics and is classified as GRAS (Generally Recognized as Safe) by the US Food and Drug Administration (FDA). This organism has remarkable biosynthetic potential [ 6 ] and secretes a variety of secondary metabolites such as enzymes and lipopeptides that exhibit broad-spectrum antimicrobial [ 7 ], anticancer [ 8 ], antinematicidal activities [ 9 ], and immune activation in plants [ 10 ] and animals ( 11 – 13 ). Lipopeptides act as immunomodulators by interacting with pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), expressed on antigen-presenting cells (macrophages and dendritic cells) to activate immune functions [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of bacillus subtilis strain Z15 secondary metabolites on immune function in mice

Cao,

Aimaier,

Yang

et al. 2023

BMC Genomics

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Background Previous studies have shown that secondary metabolites of Bacillus subtilis strain Z15 (BS-Z15) are effective in treating fungal infections in mice. To evaluate whether it also modulates immune function in mice to exert antifungal effects, we investigated the effect of BS-Z15 secondary metabolites on both the innate and adaptive immune functions of mice, and explored its molecular mechanism through blood transcriptome analysis. Results The study showed that BS-Z15 secondary metabolites increased the number of monocytes and platelets in the blood, improved natural killer (NK) cell activity and phagocytosis of monocytes-macrophages, increased the conversion rate of lymphocytes in the spleen, the number of T lymphocytes and the antibody production capacity of mice, and increased the levels of Interferon gamma (IFN-γ), Interleukin-6 (IL-6), Immunoglobulin G (IgG) and Immunoglobulin M (IgM) in plasma. The blood transcriptome analysis revealed 608 differentially expressed genes following treatment with BS-Z15 secondary metabolites, all of which were significantly enriched in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) terms for immune-related entries and pathways such as Tumor Necrosis Factor (TNF) and Toll-like receptor (TLR) signaling pathways, and upregulated expression levels of immune-related genes such as Complement 1q B chain (C1qb), Complement 4B (C4b), Tetracyclin Resistant (TCR) and Regulatory Factor X, 5 (RFX5). Conclusions BS-Z15 secondary metabolites were shown to enhance innate and adaptive immune function in mice, laying a theoretical foundation for its development and application in the field of immunity.

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“…Finally, recent studies have demonstrate the potential of new probiotic strains isolated from buffalo milk ( Bubalus bubalis ) and artisanal ferments, which have shown activity against both Gram-positive and Gram-negative pathogenic strains. Therefore, these strains could be exploited as antimicrobial probiotics for veterinary use [ 148–153 ].…”

Section: Strategies To Combat Infectionmentioning

confidence: 99%

Streptococcus iniae in aquaculture: a review of pathogenesis, virulence, and antibiotic resistance

Juárez-Cortés,

Vázquez,

Díaz

et al. 2024

International Journal of Veterinary Science and Medicine

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One of the main challenges in aquaculture is pathogenic bacterial control. Streptococcus iniae stands out for its ability to cause high mortality rates in populations of commercially important fish populations and its recent recognition as an emerging zoonotic pathogen. The rise in identifying over 80 strains some displaying antibiotic resistance coupled with the emerging occurrence of infections in marine mammal species and wild fish underscores the urgent need of understanding pathogenesis, virulence and drug resistance mechanisms of this bacterium. This understanding is crucial to ensure effective control strategies. In this context, the present review conducts a bibliometric analysis to examine research trends related to S. iniae , extending into the mechanisms of infection, virulence, drug resistance and control strategies, whose relevance is highlighted on vaccines and probiotics to strengthen the host immune system. Despite the advances in this field, the need for developing more efficient identification methods is evident, since they constitute the basis for accurate diagnosis and treatment.

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Effects of two host-associated probiotics Bacillus mojavensis B191 and Bacillus subtilis MRS11 on growth performance, intestinal morphology, expression of immune-related genes and disease resistance of Nile tilapia (Oreochromis niloticus) against Streptococcus iniae

Cited by 16 publications

References 72 publications

Influences of Bacillus subtilis and fructooligosaccharide on growth performances, immune responses, and disease resistance of Nile tilapia, Oreochromis niloticus

Influences of Bacillus subtilis and fructooligosaccharide on growth performances, immune responses, and disease resistance of Nile tilapia, Oreochromis niloticus

Effect of bacillus subtilis strain Z15 secondary metabolites on immune function in mice

Streptococcus iniae in aquaculture: a review of pathogenesis, virulence, and antibiotic resistance

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