Effects of two environmental endocrine disruptors di-n-butyl phthalate (DBP) and mono-n-butyl phthalate (MBP) on human sperm functions in vitro

Xie, Fuchen; Chen, Xuedong; Weng, Shiqi; Xia, Tianxinyu; Sun, Xinyi; Luo, Tao; Li, Peng

doi:10.1016/j.reprotox.2018.10.011

Cited by 42 publications

(17 citation statements)

References 34 publications

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“…Our data on the sensitivity of sperm to short term chemical exposure support similar studies using a range of environmentally relevant chemical challenges in vitro . For example, DEHP, DBP and mono-n-butyl phthalate (MBP), are reported to reduce sperm motility in vitro when added at seminal concentrations measured in infertile men 18,31 . A mixture of PCBs are also reported to reduce sperm motility in both the human and pig 32,33 and in the human, p,p’-dichloro-diphenyl-dichloro-ethylene (p,p’-DDE) has been shown to increase Ca 2+ uptake by sperm in a mechanism that involves the CatSper channel 34 .…”

Section: Discussionmentioning

confidence: 99%

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Sumner

Tomlinson²,

Craigon

et al. 2019

Sci Rep

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A temporal decline in human and dog sperm quality is thought to reflect a common environmental aetiology. This may reflect direct effects of seminal chemicals on sperm function and quality. Here we report the effects of diethylhexyl phthalate (DEHP) and polychlorinated biphenyl 153 (PCB153) on DNA fragmentation and motility in human and dog sperm. Human and dog semen was collected from registered donors (n = 9) and from stud dogs (n = 11) and incubated with PCB153 and DEHP, independently and combined, at 0x, 2x, 10x and 100x dog testis concentrations. A total of 16 treatments reflected a 4 × 4 factorial experimental design. Although exposure to DEHP and/or PCB153 alone increased DNA fragmentation and decreased motility, the scale of dose-related effects varied with the presence and relative concentrations of each chemical (DEHP.PCB interaction for: DNA fragmentation; human p < 0.001, dog p < 0.001; Motility; human p < 0.001, dog p < 0.05). In both human and dog sperm, progressive motility negatively correlated with DNA fragmentation regardless of chemical presence (Human: P < 0.0001, r = −0.36; dog P < 0.0001, r = −0.29). We conclude that DEHP and PCB153, at known tissue concentrations, induce similar effects on human and dog sperm supporting the contention of the dog as a sentinel species for human exposure.

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Section: Discussionmentioning

confidence: 99%

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Sumner

Tomlinson²,

Craigon

et al. 2019

Sci Rep

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“…In vivo exposure to BPA has also been reported to adversely affect sperm quality, functions and morphology of exposed mice by inducing oxidative stress and DNA damage in spermatozoa (Ikhlas & Ahmad, 2020). In vitro treatment of human spermatozoa with two different phthalates, di-butyl phthalate (DBP) and mono-n-butyl phthalate (MBP), showed a detrimental effect on sperm motility, penetration capability and capacitation along with suppressed sperm tyrosine phosphorylation, which is involved in the regulation of sperm functions (Xie et al, 2019). In vivo exposure of DBP from medications of inflammatory bowel disease (IBD) resulted in differential expression of numerous sperm RNA elements as well as activation of oxidative stress and DNA damage pathways in spermatozoa of patients with IBD (Estill, Hauser, Nassan, Moss, & Krawetz, 2019).…”

Section: Effec T Of Environmental Contaminants On S Perm Fun C Tionmentioning

confidence: 99%

“…Furthermore, alterations in the antioxidant milieu of the epididymis induced by environmental contaminants negatively affect the sperm maturation process resulting in poor sperm quality (Adewoyin et al, 2017;Rahman et al, 2019). Elevated levels of ROS cause deleterious effects on spermatozoa by inducing lipid peroxidation and sperm DNA damage resulting in poor sperm motility, morphology and function (Ikhlas & Ahmad, 2020;Rahman et al, 2015;Xie et al, 2019).…”

Section: Mechanis M Of Ac Ti On Of Environmental Contaminantsmentioning

confidence: 99%

Environmental contaminants and male infertility: Effects and mechanisms

et al. 2020

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The global incidence of infertility has risen to 15% in contrast to 7%-8% in the early 1960s (Mascarenhas, Flaxman, Boerma, Vanderpoel, & Stevens, 2012). Over the past several decades, a significant deterioration in male reproductive health has been reported in association with increased industrialisation, which has contributed to a massive release of synthetic pollutants into the environment (Rim, 2017; Skakkebaek et al., 2016). The concerns over declining male reproductive health were elicited by one of the landmark publications by Carlsen et al., which revealed a worldwide reduction in the average sperm count to half between 1940 and 1990 (Carlsen, Giwercman, Keiding, & Skakkebaek, 1992). These findings prompted several scientists, mainly Swan et al. to re-evaluate the trend in semen quality over time in different regions throughout the world and eventually confirmed a significant decrease in sperm concentration in western countries (Swan, Elkin, & Fenster, 1997). Incidentally, a parallel increase in the occurrence of testicular cancer and congenital abnormalities (cryptorchidism and hypospadias) were reported in several countries (Ferguson & Agoulnik, 2013; Hutson, 2000). These observations prompted researchers to explore the potential role of common environmental factors in causing a decline in male reproductive health. In 1993, it was hypothesised that the growing incidence of male reproductive anomalies could be due to exposure to chemicals possessing oestrogenic property (Sharpe & Skakkebaek, 1993).

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“…Versatile role of protein acetylation is obvious, including aforementioned residual histones as well as protein kinases. Taken together, the impact of endocrine disruptors on histone PTMs [23,24] as well as sperm phosphorylation [25][26][27] has been described, and, therefore, the modifications of proteins (protein PTMs) become the likely manner in which disruptors (EDs) work in their real doses.…”

Section: Endocrine Disruptors Induce Non-genomic Inheritance Through mentioning

confidence: 99%

Endocrine Disruptors: Very Low Doses with Genuinely High Impacts on Male Reproduction

Ješeta¹,

Nevoral²

2020

Male Reproductive Health

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Endocrine disruptors (EDs) are chemical substances that affect physiological processes in the body via hormonal regulation. They are often detected in food, plastic water bottles, cosmetics, and many other daily need items. Thereafter, EDs are detected in many bodily fluids, pointing out the real exposure to even very low doses. Permanent and long-term utilization of EDs has harmful effects on male reproductive health mainly due to interference with sex hormone synthesis and mechanism of action. However, with decreasing dosage of EDs, the possibilities of unpredictable modes of action arise. In addition to various molecular actions of individual EDs, the interference of individual ones represents another dimension of the ED issue. This review provides an overview of the EDs and their possible impact on reproductive health in males, with focus on sperm quality with the mighty potential of epigenetic transmission to further generations. The "posttranslational" effect of EDs in really low doses in real exposure routes is stigmatized in this review, being strongly considered as creeping molecular action of individual EDs as well as amplifications of their copresence in the environment.

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Effects of two environmental endocrine disruptors di-n-butyl phthalate (DBP) and mono-n-butyl phthalate (MBP) on human sperm functions in vitro

Cited by 42 publications

References 34 publications

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Independent and combined effects of diethylhexyl phthalate and polychlorinated biphenyl 153 on sperm quality in the human and dog

Environmental contaminants and male infertility: Effects and mechanisms

Endocrine Disruptors: Very Low Doses with Genuinely High Impacts on Male Reproduction

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