Effects of Two Different Dose Levels of Irradiation on Thioredoxin Reductase in the Kidney of Guinea Pigs

Abstract

Abstract:It is aimed to examine the state of the oxidant-antioxidant system in the liver of guinea pig caused by ionizing radiation at two different dose levels in the early period. The research was carried out on guinea pigs irradiated with the doses of 8 Gy (group 2) or 15 Gy (group 3) (single dose/whole body) in comparison with control group (group 1). The levels of thioredoxin reductase (TR) in the kidney was measured. The results obtained by non-irradiated and irradiated (8 Gy and 15 Gy) kidneys on guinea pigs indicated that; in 8-15 Gy irradiated animal kidneys TR activity elevated significantly (p<0.001) when compared to the controls, but there was no difference between 8 Gy and 15 Gy irradiated animal groups. The data have shown that gamma-irradiation results in significant increase in TR activity at two different dose levels.

Effect of the Acute Total Gamma Radiation in a Sublethal Dose on the Biophysical Properties of Red Blood Cells, Lipid Peroxidation, Antioxidant Supply and Hemocoagulating Properties of Erythrocytes

“…The experiments were performed on 11-12-week-age guinea pigs, 441.7±9.8 g weigh, males and females in equal numbers, kept separately. These animals are one of the best models, including for the study of radiation damage, since they do not synthesize ascorbic acid, similar to humans (8). Laboratory animals were kept in a vivarium of the Ukrainian Medical Stomatological Academy (UMSA is Poltava State Medical University after 05.05.2021) in a room that did not contain specific pathogens, with a natural lighting cycle at a constant temperature (21±1ºC) and humidity (50%±10%), on a standard diet.…”