Effects of two different decellularization routes on the mechanical properties of decellularized lungs

Urbano, Jéssica Julioti; Palma, Renata Kelly da; Lima, Flávia Mafra de; Fratini, Paula; Guimarães, Luciana Lopes; Uriarte, Juan J.; Alvarenga, Letícia Heineck; Miglino, María Angélica; Vieira, Rodolfo de Paula; Prates, Renato Araújo; Navajas, Daniel; Farré, Ramón; Oliveira, Luís Vicente Franco de

doi:10.1371/journal.pone.0178696

Cited by 17 publications

(17 citation statements)

References 19 publications

(34 reference statements)

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“…Different techniques and detergents (e.g. SDS, SDC, Triton-X 100 and CHAPS) have been investigated in different species, such as mouse, rat, pig and human [16,76,[101][102][103][104][105]. Unfortunately, the optimal decellularization protocol remains undefined.…”

Section: Lungmentioning

confidence: 99%

Strategies based on organ decellularization and recellularization

et al. 2019

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Summary Transplantation is the only curative treatment option available for patients suffering from end‐stage organ failure, improving their quality of life and long‐term survival. However, because of organ scarcity, only a small number of these patients actually benefit from transplantation. Alternative treatment options are needed to address this problem. The technique of whole‐organ decellularization and recellularization has attracted increasing attention in the last decade. Decellularization includes the removal of all cellular components from an organ, while simultaneously preserving the micro and macro anatomy of the extracellular matrix. These bioscaffolds are subsequently repopulated with patient‐derived cells, thus constructing a personalized neo‐organ and ideally eliminating the need for immunosuppression. However, crucial problems have not yet been satisfyingly addressed and remain to be resolved, such as organ and cell sources. In this review, we focus on the actual state of organ de‐ and recellularization, as well as the problems and future challenges.

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Section: Lungmentioning

confidence: 99%

Strategies based on organ decellularization and recellularization

et al. 2019

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“…[4][5][6][7][8][9][10][11][12] Some authors have reported acceptable degrees of decellularization using other detergents such as sodium dodecyl sulfate (SDS), Triton X-100 or 3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate (CHAPS) alone or in combination. [13][14][15][16][17][18][19][20] However, in many cases the authors have directly extrapolated the decellularization protocols reported in the literature, without considering important aspects such as differences in the composition of the extracellular matrix that exist between species.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

et al. 2021

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Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

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“…As a result, coating of collagen hydrogel (Fig. 1A2a-A2b) provided an additional nanofibrous structure, interpenetrating throughout the interconnected printed network, mimicking more closely the characteristic structure and function of the extracellular matrix of trachea [32,33].…”

Section: Morphology and Stiffness Softening Of 3d-tips Hybrid Scaffoldsmentioning

confidence: 97%

Human airway-like multilayered tissue on 3D-TIPS printed thermoresponsive elastomer/collagen hybrid scaffolds

Magaz

Huo

et al. 2020

Acta Biomaterialia

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Developing a biologically representative complex tissue of the respiratory airway is challenging, however, beneficial for treatment of respiratory diseases, a common medical condition representing a leading cause of death in the world. This study reports a successful development of synthetic human tracheobronchial epithelium based on interpenetrated hierarchical networks composed of a reversely 3D printed porous structure of a thermoresponsive stiffness-softening elastomer nanohybrid impregnated with collagen nanofibrous hydrogel in vitro. Human bronchial epithelial cells (hBEpiCs) were able to attach and grow into an epithelial monolayer on the hybrid scaffolds co-cultured with either human bronchial fibroblasts (hBFs) or human bone-marrow derived mesenchymal stem cells (hBM-MSCs), with substantial enhancement of mucin expression, ciliation, well-constructed intercellular tight junctions and adherens junctions. The multi-layered co-culture 3D scaffolds consisting of a top monolayer of differentiated epithelium, with either hBFs or hBM-MSCs proliferating within the hyperelastic nanohybrid scaffold underneath, created a tissue analogue of the upper respiratory tract, validating these 3D printed guided scaffolds as a platform to support co-culture and cellular organization. In particular, hBM-MSCs in the co-culture system promoted an overall matured physiological tissue analogue of the respiratory system, a promising synthetic tissue for drug discovery, tracheal repair and reconstruction.

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Effects of two different decellularization routes on the mechanical properties of decellularized lungs

Cited by 17 publications

References 19 publications

Strategies based on organ decellularization and recellularization

Strategies based on organ decellularization and recellularization

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

Human airway-like multilayered tissue on 3D-TIPS printed thermoresponsive elastomer/collagen hybrid scaffolds

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