Effects of two dietary protein levels on growth, body composition, intestinal microflora and expression of TOR, IGF‐I, LPL and HSP70 of juvenile silver sillago,<i>Sillago sihama</i>

Liu, Hao; Dong, Xiaohui; Tan, Beiping; Du, Tao; Zhang, Shuang; Yang, Yefeng; Chi, Shuyan; Liu, Hongyu

doi:10.1111/anu.13357

Cited by 3 publications

(4 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The microbiota in our intestines plays a crucial role in maintaining overall health, and dietary components can influence the microbial community and subsequently impact the metabolism and population of important commensal bacteria [ 20 ]. Moryella , a Gram-positive, non-spore-forming, strictly anaerobic, and non-motile genus of bacteria from the Lachnospiraceae family [ 58 ], was found to be significantly more abundant in the high-dose AFB1 group in our experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Observation. Histological analyses were conducted using the methodology outlined in prior research [20]. The hindgut and muscle samples were immersed in Bouin's solution for 24 hr to fix them, and then preserved in ethanol with a concentration of 700 g kg −1 .…”

Section: Hindgut and Muscle Histologicalmentioning

confidence: 99%

“…Analysis of the Whole-Intestinal Microbiota. Following the manufacturer's directions, the TGuide S96 Magnetic Soil/ Stool DNA Kit (Tiangen Biotech (Beijing) Co., Ltd.) was used to get total genomic DNA from intestine samples [20]. The area V3-V4 of the bacterial 16S rRNA gene that changes a lot was amplified using primer pairs 338 F: 5 ′ -ACTCCTACGGGAGGCAGCA−3 ′ and 806R: 5 ′ -GGAC-TACHVGGGTWTCTAAT−3 ′ .…”

Section: 7mentioning

confidence: 99%

See 2 more Smart Citations

Effects of Dietary Aflatoxin B1 on Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Growth, Intestinal Health, and Muscle Quality

Liu,

Xie,

Huang

et al. 2024

Aquaculture Nutrition

Self Cite

View full text Add to dashboard Cite

This study investigated the effects of varying doses of dietary aflatoxin B1 (AFB1) on the growth, intestinal health, and muscle quality of hybrid grouper. Four diets with varying AFB1 concentrations (0, 30, 445, and 2,230 μg kg−1) were used. Elevating AFB1 concentrations led to a decline in growth indexes, specifically the weight gain rate and the specific growth rate, although the survival rate remained unchanged. Morphological indicators showed a dose-dependent decline with AFB1 exposure. Intestinal MDA content and hindgut reactive oxygen species (ROS) levels increased, while antioxidant indexes and digestive enzymes decreased with higher AFB1 levels. AFB1 negatively influenced hindgut tight junction protein and antioxidant-related gene expression while promoting inflammation-related gene expression. The presence of AFB1 in the experiment led to a decrease in beneficial intestinal bacteria, such as Prevotella, and an increase in harmful intestinal bacteria, such as Prevotellaceae_NK3B31_group. Muscle lipid and unsaturated fatty acid content significantly decreased, while muscle protein and liver AFB1 content increased dramatically with higher AFB1 concentrations. AFB1 caused myofibrillar cleavage and myofilament damage, leading to increased spaces between muscle fibers. In conclusion, diets with AFB1 levels exceeding 30 μg kg−1 inhibited hybrid grouper growth, while levels surpassing 445 μg kg−1 resulted in hindgut ROS accumulation, inflammation, elevated intestinal permeability, reduced digestive enzyme activity, and compromised muscle quality.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Hindgut and Muscle Histologicalmentioning

confidence: 99%

Section: 7mentioning

confidence: 99%

See 1 more Smart Citation

Effects of Dietary Aflatoxin B1 on Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Growth, Intestinal Health, and Muscle Quality

Liu,

Xie,

Huang

et al. 2024

Aquaculture Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many previous studies have demonstrated that variations in dietary nutrient levels significantly affect the structure of the intestinal microbiota of shrimp. Liu et al investigated the effects of two protein levels on silver pomfret larvae and observed significant effects on the structure of the intestinal microbiota [47]. Fan et al also observed that the composition of the intestinal microbiota was significantly affected after using cottonseed protein to de-substitute different levels of fish meal as a dietary protein [48].…”

Section: Discussionmentioning

confidence: 99%

Investigating the Impact of Varied Dietary Protein Levels on Litopenaeus vannamei: An Exploration of the Intestinal Microbiota and Transcriptome Responses

Li,

Yuan,

et al. 2024

Animals

Self Cite

View full text Add to dashboard Cite

This study explored the effects of dietary protein levels on Litopenaeus vannamei with its intestinal microbiota and transcriptome responses. Previous studies on the effects of dietary protein levels on L. vannamei have focused on growth performance, antioxidant indices, and digestive enzyme activity, but few studies have been conducted at the microbiological and molecular levels. In this study, five isolipid experimental diets with protein levels of 32% (P32), 36% (P36), 40% (P40), 44% (P44), and 48% (P48) were used in an L. vannamei (0.63 ± 0.02 g) feeding trial for 56 days. At the end of the feeding trial, the growth performance, immunity, intestinal health, and transcriptional responses of L. vannamei were determined. This study demonstrated that higher protein levels (P44) led to superior weight gain and growth rates for L. vannamei, with lower feed conversion ratios (FCR) observed in the P48 and P44 groups compared to the P32 and P36 groups (p ≤ 0.05). The P44 and P48 groups also showed a notably higher protein efficiency ratio (PER) compared to others (p ≤ 0.05), and there was no significant difference between them. Upon Vibrio parahaemolyticus infection, the P48 group exhibited a significantly lower survival rate (SR) within 48 h, while during 72 h of white spot syndrome virus (WSSV) infection, the P44 group had a notably higher survival rate than the P32 group (p ≤ 0.05). Digestive enzyme activity and antioxidant levels in L. vannamei initially increased and then decreased as protein levels increased, usually peaking in the P40 or P44 groups. Lower dietary protein levels significantly reduced the relative abundance of beneficial bacteria and increased the relative abundance of pathogenic bacteria in the intestines of L. vannamei. Transcriptome sequencing analysis revealed that most differentially expressed genes (DEGs) were up-regulated and then down-regulated as dietary protein levels increased. Furthermore, KEGG pathway enrichment analysis indicated that several immune and metabolic pathways, including metabolic pathways, glutathione metabolism, cytochrome P450, and lysosome and pancreatic secretion, were significantly enriched. In summary, the optimal feed protein level for L. vannamei shrimp was 40–44%. Inappropriate feed protein levels reduced antioxidant levels and digestive enzyme activity and promoted pathogen settlement, deceasing factors in various metabolic pathways that respond to microorganisms through transcriptional regulation. This could lead to stunted growth in L. vannamei and compromise their immune function.

show abstract

A study of the potential effect of dietary fishmeal replacement with cricket meal (Gryllus bimaculatus) on growth performance, blood health, liver antioxidant activities, intestinal microbiota and immune-related gene expression of juvenile channel catfish

Fan

Liu

Pei

et al. 2023

Animal Feed Science and Technology

View full text Add to dashboard Cite

Effects of two dietary protein levels on growth, body composition, intestinal microflora and expression of TOR, IGF‐I, LPL and HSP70 of juvenile silver sillago,Sillago sihama

Cited by 3 publications

References 51 publications

Effects of Dietary Aflatoxin B1 on Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Growth, Intestinal Health, and Muscle Quality

Effects of Dietary Aflatoxin B1 on Hybrid Grouper (Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂) Growth, Intestinal Health, and Muscle Quality

Investigating the Impact of Varied Dietary Protein Levels on Litopenaeus vannamei: An Exploration of the Intestinal Microbiota and Transcriptome Responses

A study of the potential effect of dietary fishmeal replacement with cricket meal (Gryllus bimaculatus) on growth performance, blood health, liver antioxidant activities, intestinal microbiota and immune-related gene expression of juvenile channel catfish

Contact Info

Product

Resources

About