Effects of two chronic stresses on mental state and hair follicle melanogenesis in mice

Abstract

Growing bodies of data show that psychological stress can be associated with hair loss and vitiligo. Researchers have revealed that stress could indeed inhibit hair growth in vivo, but the relationship between chronic stress and melanogenesis remains unknown. In this study, we established two types of stress models, chronic restraint stress (CRS) and chronic unpredicted mild stress (CUMS) mice models, and explored the possible role of stress in mice hair follicle melanogenesis. We found that stress changed hippocampal morphology, decreased 5-HT level in brain and skin and down-regulated 5-HT1A receptor expression in hippocampal CA1 region and skin. The alterations of 5-HT and 5-HT1A receptor might be a threshold of central stress to associate with the behaviour changes. Both two stresses caused cellular damage of melanocytes and inhibition of keratinocytes proliferation in HF, which made the synthetic pigment loss. CRS which was considered primarily as a "psychological" stressor had the lower melanin production in HF, as well as the level of 5-HT in skin was down-regulated more than those in CUMS group.

HSP70iQ435A-Encoding DNA Repigments Vitiligo Lesions in Sinclair Swine

“…This aligns with increased depigmentation in stressed porcine skin, and further supports the use of spontaneously depigmenting swine for therapeutic assessments (Bourneuf, 2017). Emotional stress may likewise contribute to vitiligo in this model as an impact of stress on depigmentation was recently shown in mice (Liao et al, 2017).…”

Isoliquiritigenin inhibits melanogenesis, melanocyte dendricity and melanosome transport by regulating ERK‐mediated MITF degradation

“…The membranes were blocked for 1.5 hours at 25°C with 3% BSA in TBST, and then incubated with the suitable antibodies overnight at 4°C. The blots were then incubated with peroxidase‐conjugated secondary antibodies for 1 hour at 25°C, and visualized by using enhanced chemiluminescence . The Western blot analysis results reported herein are representative of at least three experiments.…”

“…The blots were then incubated with peroxidase-conjugated secondary antibodies for 1 hour at 25°C, and visualized by using enhanced chemiluminescence. [32] The Western blot analysis results reported herein are representative of at least three experiments.…”

Protoporphyrin IX Stimulates Melanogenesis, Melanocyte Dendricity, and Melanosome Transport Through the cGMP/PKG Pathway

“…Subsequently, the membranes were blocked with blocking buffer containing 2.5% bovine serum albumin (BSA) in Tris-buffered saline and 0.1% Tween 20 (TBST) for 1.5 h at 25°C and incubated with the corresponding primary antibodies overnight at 4°C. Next, the blots were incubated with peroxidase-conjugated secondary antibodies for 1 h at 25°C and visualized using enhanced chemiluminescence (Liao et al, 2017;Yun et al, 2020). The western blot results shown here are representative of three experiments.…”