Effects of Two Bacillus Velezensis Microbial Inoculants on the Growth and Rhizosphere Soil Environment of Prunus davidiana

Abstract

Microbial inoculants, as harmless, efficient, and environmentally friendly plant growth promoters and soil conditioners, are attracting increasing attention. In this study, the effects of Bacillus velezensis YH-18 and B. velezensis YH-20 on Prunus davidiana growth and rhizosphere soil bacterial community in continuously cropped soil were investigated by inoculation tests. The results showed that in a pot seedling experiment, inoculation with YH-18 and YH-20 resulted in a certain degree of increase in diameter growth, plant height, and leaf area at different time periods of 180 days compared with the control. Moreover, after 30 and 90 days of inoculation, the available nutrients in the soil were effectively improved, which protected the continuously cropped soil from acidification. In addition, high-throughput sequencing showed that inoculation with microbial inoculants effectively slowed the decrease in soil microbial richness and diversity over a one-month period. At the phylum level, Proteobacteria and Bacteroidetes were significantly enriched on the 30th day. At the genus level, Sphingomonas and Pseudomonas were significantly enriched at 15 and 30 days, respectively. These bacterial phyla and genera can effectively improve the soil nutrient utilization rate, antagonize plant pathogenic bacteria, and benefit the growth of plants. Furthermore, inoculation with YH-18 and inoculation with YH-20 resulted in similar changes in the rhizosphere microbiome. This study provides a basis for the short-term effect of microbial inoculants on the P. davidiana rhizosphere microbiome and has application value for promoting the cultivation and production of high-quality fruit trees.

Paenibacillus polymyxa and Bacillus cereus co-bacterial agent promotes ginseng resistance to diseases and improves yield, quality and pesticide degradation through regulating rhizosphere microbial community structure and potential function

“…According to the report, Pseudomonas and Bacillus co-bacterial agent promotes the growth of soybean and increases its yield [51]. The application of a mixture of Bacillus YH-18 and YH-20 can effectively improve the nutrient utilisation of continuous cropping soils, antagonise the pathogenic bacteria of plants, and bene t the plant growth [52]. Mixed bioinoculant MI of Bacillus, Pseudomonas, Streptomyces, and Trichoderma has the potential to enhance wheat growth and seed yield [53].…”

Serratia marcescens LYGN1 Reforms the Rhizosphere Microbial Community and Promotes Cucumber and Pepper Growth in Plug Seedling Cultivation

“…Previous studies showed that the application of PGPR could promote plant growth by reforming the rhizosphere microbial environment [ 6 , 34 ]. In this study, we showed that LYGN1 treatment has the same trends.…”

Effective microorganisms input efficiently improves the vegetation and microbial community of degraded alpine grassland

“…The high addition of EM, such as M2 and M3, resulted in a significantly higher amount of soil microorganisms, bacteria, and fungi compared to the CK, particularly in 2019 ( Figure 2 ). The addition of EM might result in competition with those indigenous microorganisms and hinder the growth and development of specific pathogens ( Mallon et al, 2015 ; Shi et al, 2022 ). All of these activity would cause alterations in the composition of microorganisms in the soil.…”