Effects of Tween 80 on cellulase stability under agitated conditions

Okino, Shohei; Ikeo, Makoto; Ueno, Yoshiki; Taneda, Daisuke

doi:10.1016/j.biortech.2013.05.078

Cited by 51 publications

(24 citation statements)

References 22 publications

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“…As presenças de Tween 20 e Tween 80 podem eliminar a desativação enzimática atribuída à lignina, devido à exclusão das enzimas da superfície da lignina, além da atuação na dessorção das celulases dos substratos durante a sacarificação, o que aumenta o rendimento da mesma. 192,198,199,205 O estudo do efeito da adição de Tween 80 na hidrólise da palha de milho realizada por enzimas comerciais permitiu compreender melhor o papel da lignina na redução da adsorção das celulases sobre o substrato, em parte devido à adsorção do surfactante que ocupava a superfície hidrofóbica da lignina da palha de milho. 206 Em resumo, o uso de aditivos mais comumente encontrados na literatura (proteínas não catalíticas, polímeros e surfactantes não-iônicos), assim como a proteína de soja, que vem sendo testada como uma alternativa mais economica, é significativamente eficaz e reduz a adsorção improdutiva das enzimas do complexo celulolítico sobre a lignina, além de proteger as enzimas da desnaturação térmica durante o processo de hidrólise.…”

Section: Adsorção Improdutiva De Enzimas Na Ligninaunclassified

Current challenges on the production and use of cellulolytic enzymes in the hydrolysis of lignocellulosic biomass

Florencio¹,

Badino²,

Farinas³

2017

Quim. Nova

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The second generation (2G) ethanol obtained from lignocellulosic biomass has been considered as a potential alternative source of energy for the transportation sector. However, the technologies to convert lignocellulose into fermentable sugars still face several challenges. One of the major challenges of this process is related to the cost of the cellulolytic enzymes. Thus, the development of bioprocesses for enzyme production and strategies to increase the final yield of the enzymatic hydrolysis reactions are required to ensure the economic feasibility of the biomass conversion process. In this review, it is discussed the progress related to fungal enzyme technology over the past few years with major emphasis on the biocatalysts needed for cellulose degradation as well as on cultivation strategies for the production of enzymes on-site. The potential of applying secretome analysis as a tool for the characterization of the enzymatic complexes and some limitations on the use of enzymatic cocktails in the saccharification step, such as the unproductive adsorption of enzymes onto lignin are also discussed in light of the recent literature.

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Section: Adsorção Improdutiva De Enzimas Na Ligninaunclassified

Current challenges on the production and use of cellulolytic enzymes in the hydrolysis of lignocellulosic biomass

Florencio¹,

Badino²,

Farinas³

2017

Quim. Nova

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“…The exact role of Tween 80 cannot be concluded from these results but the role of this surfactant could be versatile. In addition to increasing oxygen transfer, stabilization of cellulases (Okino et al, ) and protection from unproductive binding to cellulose (Helle et al, ) are some of the effects reported in literature. From Figure , it is clear that enzyme performance can be increased or decreased drastically depending on the cultivation parameters (pH, temperature, and agitation) chosen during the production of the enzymes.…”

Section: Resultsmentioning

confidence: 99%

Linking hydrolysis performance to Trichoderma reesei cellulolytic enzyme profile

Lehmann

Rønnest

Jørgensen

et al. 2015

Biotech & Bioengineering

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Trichoderma reesei expresses a large number of enzymes involved in lignocellulose hydrolysis and the mechanism of how these enzymes work together is too complex to study by traditional methods, for example, by spiking with single enzymes and monitoring hydrolysis performance. In this study, a multivariate approach, partial least squares regression, was used to see whether it could help explain the correlation between enzyme profile and hydrolysis performance. Diverse enzyme mixtures were produced by T. reesei Rut-C30 by exploiting various fermentation conditions and used for hydrolysis of washed pretreated corn stover as a measure of enzyme performance. In addition, the enzyme mixtures were analyzed by liquid chromatography-tandem mass spectrometry to identify and quantify the different proteins. A multivariate model was applied for the prediction of enzyme performance based on the combination of different proteins present in an enzyme mixture. The multivariate model was used for identification of candidate proteins that are correlated to enzyme performance on pretreated corn stover. A very large variation in hydrolysis performance was observed and this was clearly caused by the difference in fermentation conditions. Besides β-glucosidase, the multivariate model identified several xylanases, Cip1 and Cip2, as relevant proteins to study further.

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“…2 and 3). Furthermore, Tween 80 can also improve the cellulase stability and hydrolysis efficiency of lignocellulose (Okino et al, 2013) and thereby lead to higher ethanol production and enzyme productivity regardless of enzyme whether recycling strategy A or strategy C is used (Fig. 6).…”

Section: Discussionmentioning

confidence: 99%

Enhanced cellulase recovery without β‐glucosidase supplementation for cellulosic ethanol production using an engineered strain and surfactant

Huang

Guo

et al. 2016

Biotech & Bioengineering

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Recycling cellulases by substrate adsorption is a promising strategy for reducing the enzyme cost of cellulosic ethanol production. However, β-glucosidase has no carbohydrate-binding module (CBM). Thus, additional enzymes are required in each cycle to achieve a high ethanol yield. In this study, we report a new method of recycling cellulases without β-glucosidase supplementation using lignocellulosic substrate, an engineered strain expressing β-glucosidase and Tween 80. The cellulases and Tween 80 were added to an aqueous suspension of diluted sulfuric acid/ammonia-treated corncobs in a simultaneous saccharification and fermentation (SSF) process for ethanol production. Subsequently, the addition of fresh pretreated corncobs to the fermentation liquor and remaining solid residue provided substrates with absorbed cellulases for the next SSF cycle. This method provided excellent ethanol production in three successive SSF cycles without requiring the addition of new cellulases. For a 10% (w/v) solid loading, a cellulase dosage of 30 filter paper units (FPU)/g cellulose, 0.5% Tween 80, and 2 g/L of the engineered strain, approximately 90% of the initial ethanol concentration from the first SSF process was obtained in the next two SSF processes, with a total ethanol production of 306.27 g/kg corncobs and an enzyme productivity of 0.044 g/FPU. Tween 80 played an important role in enhancing cellulase recovery. This new enzyme recycling method is more efficient and practical than other reported methods. Biotechnol. Bioeng. 2017;114: 543-551. © 2016 Wiley Periodicals, Inc.

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Effects of Tween 80 on cellulase stability under agitated conditions

Cited by 51 publications

References 22 publications

Current challenges on the production and use of cellulolytic enzymes in the hydrolysis of lignocellulosic biomass

Current challenges on the production and use of cellulolytic enzymes in the hydrolysis of lignocellulosic biomass

Linking hydrolysis performance to Trichoderma reesei cellulolytic enzyme profile

Enhanced cellulase recovery without β‐glucosidase supplementation for cellulosic ethanol production using an engineered strain and surfactant

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