Effects of Tritrichomonas muris on the Mouse Intestine: A Proteomic Analysis

Abstract

Abstract:Although Tritrichomonas muris is a common parasite often detected in experimental animals including mice, its pathogenesis in host animals remains unclear. Proteomics can be used to specifically analyze biochemical host-parasite interaction and immune responses of the host to parasites. However, proteomics have not yet been applied to T. muris studies. Here, the effects of T. muris on the host were analyzed by proteomics. We found that 10 different proteins were expressed in T. muris-infected mice intestines compared with noninfected intestines. The identified proteins represented several functions mainly related to stress, immune response, metabolism and signal transduction. The results suggest that T. muris infection may affect processes that are acclimatizing to the environmental changes caused by the infection in the mouse intestine.

Fucose Ameliorates Tritrichomonas sp.-Associated Illness in Antibiotic-Treated Muc2−/− Mice

“…Tritrichomonas spp. are known to affect host physiology by changing the gut proteome [ 66 ] and triggering immune responses in the intestine [ 67 , 68 ]. In the previous studies, the mice were not tested for presence of protozoan microorganisms [ 42 , 43 ], so we assumed Tsp.…”

Fingerprinting and Genetic Relationship of Trichomonas Vaginalis, Trichomonas Muris and Trichomonas Gallinae

“…These studies produced different results, even when using similar techniques, in attempting to demonstrate concordance between parasite genotypes and phenotypic expressions during infection. (Kashiwagi et al, 2009) confirmed that 10 different proteins were expressed in T. muris infected mice intestines compared to non-infected mice intestines. The identified proteins represented several functions mainly related to stress, immune response, metabolism and signal transduction.…”

Proteomics and Host–Pathogen Interactions

“…40,124 Other studies have also highlighted the pathogenic changes in the brain of SIV-infected monkeys, 125 adaptive metabolic changes in Trypanosoma cruzi and Trypanosoma congolense, 126,127 or molecular biomarkers of intestinal disorder induced by H. pylori or Tritrichomonas muris. 100,128 Subsequently, the use of model organisms interacting with infectious agent of medical importance emphasized the complexity and pathogen specificity of the worm's immune response. 129 Taken together, these examples demonstrate the potential of the concept of pathogeno-proteomics and promote this new research avenue.…”