Effects of Trichosanthis Radix Extract on Collagen Production

Abstract

This study was conducted to study the effects of Trichosanthis Radix water extract (TR-W) and Trichosanthis Radix 60% ethanol extract (TR-E) on collagen production. As a result of conducting a cytotoxicity experiment on HDFn cells and RAW 264.7 cells, the experiment was conducted at a concentration of TR-W and TR-E at 100 mg/ml, which does not significantly affect the survival rate in HDF and RAW 264.7 cells. The effect on collagen synthesis ability showed an increase of 52% for the positive control group L-AA (vitamin C) 52% for TR-W 25% and TR-E 36% at 100 mg/ml proving its effect on collagen synthesis ability. In terms of inhibition of MMP-1 expression related to collagen damage L-AA (vitamin C) a positive control group, showed an inhibitory effect of 54%, and TR-W and TR-E showed an inhibitory effect of 72% and 68% at 100 mg/ml, thereby inhibiting collagen decomposition enzyme. MMP-1 was stably inhibited. Compared to Control, the free radical scavenging activity of L-AA (vitamin C) a positive control group, showed a scavenging activity of 108.4%, and in TR-W and TR-E, it showed an inhibitory effect of 93.4% and 98.2% at 100 mg/ml. In NO related to inflammation, showed an anti-inflammatory effect of 67.4% and 50.2% at a concentration of 100 mg/ml of TR-W and TR-E compared to the control group, which is expected to have a positive effect on collagen production due to inflammation. This study The results are judged to have the highest utility value as a functional cosmetic material considering the stability of the skin in the future.