Effects of tributyltin on defense-related activities of oyster hemocytes

Fisher, William S.; Wishkovsky, A.; Chu, Fu‐Lin E.

doi:10.1007/bf01054978

Cited by 68 publications

(26 citation statements)

References 23 publications

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“…Alterations in immunocompetence have been reported for bivalves after exposure, both in vitro and in vivo, to organic and inorganic contaminants, such as heavy metals (Cheng & Sullivan 1984, Cheng 1988, Viarengo et al 1994, Pipe et al 1999, Matozzo et al 2001, Sauvé et al 2002, organotins (Fisher et al 1990, Auffret & Oubella 1997, Cima et al 1998, Matozzo et al 2002, fungicides (Alvarez & Friedl 1992), polycyclic aromatic hydrocarbons (PAHs) (Sami et al 1992, Coles et al 1994, Grundy et al 1996, Gómez-Mendikute et al 2002 and chlorinated phenols (Florence et al 1997). Among the functional responses of bivalve circulating cells, total haemocyte count (THC), viability, motility, aggregation and adhesion capability, phagocytosis, production of reactive oxygen species (ROS), hydrolytic and oxidative enzyme activities, and lysosomal and cell membrane stability are all generally recognised as useful biomarkers in immunotoxicity studies.…”

Section: Introductionmentioning

confidence: 99%

4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

Matozzo¹,

Marin

2005

Mar. Ecol. Prog. Ser.

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The effects of 4-nonylphenol (NP) on functional responses of haemocytes from the clamTapes philippinarum were investigated after 7 d exposure to sublethal NP concentrations (0, 0 + acetone, 0.025, 0.05, 0.1 and 0.2 mg l -1 NP). Haemocytes from both controls and exposed clams were collected, and the effects of NP on uptake of the vital dye Neutral Red (NR), superoxide dismutase (SOD) and lysozyme activities, total haemocyte count (THC) and volume of circulating cells were evaluated. The capability of NP to induce apoptosis was also investigated. Exposure of clams to 0.2 mg l -1 NP significantly increased (p < 0.05) NR uptake when compared with controls, suggesting that NP caused alterations in cell membrane stability. Significant decreases in both SOD and lysozyme activity were observed from 0.05 mg l -1 NP (p < 0.01 and p < 0.05, respectively) with respect to controls, indicating that NP causes oxidative stress and reduces the immunocompetence of the exposed clams. From 0.05 mg l -1 NP, the apoptotic index (percentage of haemocytes showing positivity to the TUNEL reaction) significantly increased (p < 0.001). Apoptotic haemocytes showed shrinkage and lost their amoeboid shape. Moreover, NP exposure significantly increased THC (p < 0.05 at 0.2 mg l -1 NP) and caused a different distribution in size frequency. On the basis of the threshold concentration values, the most sensitive endpoints for NP were SOD, lysozyme and apoptosis assays. Our results highlight a relationship between NP exposure and changes in the functional responses of haemocytes, suggesting that the contaminant induces immunomodulation in T. philippinarum, mainly by altering cell morphology, cell membrane stability and enzymatic activities, and by promoting oxidative stress and apoptotic events.

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Section: Introductionmentioning

confidence: 99%

4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

Matozzo¹,

Marin

2005

Mar. Ecol. Prog. Ser.

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“…Chemiluminescence (an indirect indicator of phagocytosis), locomotion of hemocytes and time to spread were shown to be modulated by in vitro exposure to TBT (Fisher et al 1990). Furthermore, Fisher et al (1995) showed the virulence of the protozoan pathogen Perkinsus marinus in the oyster Crassostrea virginica to be correlated to TBT exposure.…”

Section: Introductionmentioning

confidence: 99%

Hemocyte functions and bacterial clearance affected in vivo by TBT and DBT in the blue mussel Mytilus edulis

St-Jean¹,

Pelletier²,

Courtenay³

2002

Mar. Ecol. Prog. Ser.

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“…This research field is hampered by the limited knowledge of ecological impacts following exposure to hazardous chemical pollutants. However, the detrimental biological effects of tributyltin (TBT) on marine biota are well recognized (e.g., Bryan et al 1987;Fisher et al 1990) and negative impacts of TBT pollution on the marine environment in the Baltic Sea have been documented (Eklund et al 2008). TBT has previously been used in a risk-based analysis by Moore and Postle (1994), but the authors were unable to include non-market values in the impacts caused by the compound.…”

Section: Introductionmentioning

confidence: 99%

Valuation of ecotoxicological impacts from tributyltin based on a quantitative environmental assessment framework

Noring

Håkansson

Dahlgren

2015

Ambio

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In the scientific literature, few valuations of biodiversity and ecosystem services following the impacts of toxicity are available, hampered by the lack of ecotoxicological documentation. Here, tributyltin is used to conduct a contingent valuation study as well as costbenefit analysis (CBA) of measures for improving the environmental status in Swedish coastal waters of the Baltic Sea. Benefits considering different dimensions when assessing environmental status are highlighted and a quantitative environmental assessment framework based on available technology, ecological conditions, and economic valuation methodology is developed. Two scenarios are used in the valuation study: (a) achieving good environmental status by 2020 in accordance with EU legislation (USD 119 household -1 year -1 ) and (b) achieving visible improvements by 2100 due to natural degradation (USD 108 household -1 year -1 ) during 8 years. The later scenario was used to illustrate an application of the assessment framework. The CBA results indicate that both scenarios might generate a welfare improvement.

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Effects of tributyltin on defense-related activities of oyster hemocytes

Cited by 68 publications

References 23 publications

4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

4-Nonylphenol induces immunomodulation and apoptotic events in the clam Tapes philippinarum

Hemocyte functions and bacterial clearance affected in vivo by TBT and DBT in the blue mussel Mytilus edulis

Valuation of ecotoxicological impacts from tributyltin based on a quantitative environmental assessment framework

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