Effects of trehalose vitrification and artificial oocyte activation on the development competence of human immature oocytes

Zhang, Zhiguo; Wang, Tianjuan; Hao, Yan; Panhwar, Fazil; Chen, Zhongrong; Zou, Weiwei; Ji, Dongmei; Chen, Beili; Zhou, Ping; Zhao, Gang; Cao, Yunxia

doi:10.1016/j.cryobiol.2016.12.004

Cited by 27 publications

(30 citation statements)

References 45 publications

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“…Whatever, these suggest that FF AMH may be involved in follicular aging process. Sacha et al found higher FF AMH appears to be associated with a signi cantly higher probability of clinical pregnancy,but in their study, the patients were not grouped by age [24]. In our study, patients were grouped by age and ROC curve showed that predictive value of follicular uid AMH levels for probability of clinical pregnancy was signi cant just in old age group.…”

Section: Discussioncontrasting

confidence: 55%

“…Moreover, we do not measure all tubes of follicular uid AMH levels in each patient on the day of oocyte retrieval. Although follicular uid AMH concentrations show little variability between pre-ovulatory follicles in a retrospective cohort study [24], analyzing relationship between individual cumulus-oocyte complex and corresponding follicular uid AMH levels might lead to more reliable results. Last but not least, TERT measurement with qPCR in the study is at the levels of transcription, other methods of TERT assessment such as western blot can be performed at translation level so as to con rm the results.…”

Section: Discussionmentioning

confidence: 98%

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Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Hao

Jing

et al. 2021

Preprint

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Background: Telomere attrition has been shown to play a critical role in the reproductive aging process in human beings. Telomere length (TL) is normally regulated by telomerase enzyme. Telomerase reverse transcriptase (TERT) is the main component of the telomerase. Anti-Mullerian hormone (AMH), a member of the transforming growth factor superfamily, is derived from the granulosa cells of early developing pre-antral and antral follicles. The aim of this study was to evaluate the associations between relative telomere length (RTL), TERT expression of granulosa cells (GC), follicular fluid (FF) AMH levels and ovarian/embryonic performance in infertile women at different age. Moreover, whether they acting as predictors for probability of clinical pregnancy were also assessed. Method: A total of 160 women underwent their ﬁrst fresh cycle of in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) were included in our study as follows: 100 women were enrolled for RTL measurement and 60 women were enrolled for TERT measurement. All these 160 women underwent FF AMH measurement. Correlations between RTL，TERT expression, FF AMH levels and age, ovarian/embryonic performance and probability of clinical pregnancy were assessed.Results: There was a statistically significant relationship between the expression levels of TERT, RTL, FF AMH levels and patient age(r = −0.20, P = 0.04; r=0.30, P=0.02; r=-0.191, P=0.003, respectively). Relationships between the expression levels of TERT, FF AMH levels and oocytes yield were significant (P<0.001; P<0.05, respectively). However, no statistically correlation was observed between the RTL of GC samples and oocytes yield. All these three biomarkers had no correlation with blastocyst formation rate. There was significant relationship between FF AMH levels and probability of clinical pregnancy in patients older than 35 years (OR=1.284, 95%CI=1.031-1.599, P=0.026). Conclusion: RTL, relative TERT expression in GC and AMH levels in follicular fluid are age-related, but all of them fail to predict embryonic outcomes. Relative TERT expression and FF AMH levels appear to be more reliable for prediction of ovarian response than RTL. FF AMH is also a good predictor for probability of clinical pregnancy in advanced women.

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Section: Discussioncontrasting

confidence: 55%

Section: Discussionmentioning

confidence: 98%

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Hao

Jing

et al. 2021

Preprint

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“…Some other reagents and/or methods have been applied to achieve AOA, such as electric current (31,32), ethanol (33), strontium chloride (34), and puromycin (8). There are still very limited reports on comparison of different activation reagents on human oocyte activation and embryo development (8,31,34) but it appears that calcium ionophore has better efficiency than strontium chloride (34).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Niu¹,

Ruan²,

Witz

et al. 2020

Front. Endocrinol.

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Fertilization failure is common in patients with round-headed sperm, a form of globozoospermia. Artificial oocyte activation is able to assist oocyte fertilization after sperm injection in these patients. Comparisons between oocyte fertilization with or without calcium ionophore have been reported in patients with round-headed sperm. However, no comparison has been reported between round-headed sperm injection followed by calcium ionophone activation and normal sperm injection. In this case report, half of oocytes from a patient were injected with her partner's round-headed sperm followed by calcium ionophore activation, and the other half of oocytes were injected with a donor sperm without calcium ionophore activation. The injected oocytes were cultured to examine fertilization, embryo development, and embryonic aneuploidies in the resulting blastocysts. The fertilization rate was lower in round-headed sperm injected oocytes (3/6) than that in donor sperm injected oocytes (5/6), but rates of blastocyst and aneuploidies were similar in the resulting embryos between the two groups. A euploid blastocyst resulted from round-headed sperm injection was transferred, and a healthy baby was delivered. These results indicate that calcium ionophore treatment can assist oocyte activation in patients with round-headed sperm, but its efficiency to activate oocytes is lower than that induced by a normal sperm injection. However, embryo development and chromosome integrity may not be affected by calcium ionophore treatment.

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“…Owing to multiple cryoprotective properties, selected disaccharides, such as sucrose and trehalose, are widely used in cryopreservation practice as additives to various freezing [2,51] and vitrification solutions [15,33,83]. Conceptually, combined introduction of sugars into cryopreservation media and their pre-freeze loading into cells serves as an alternative to conventional cryopreservation workflow.…”

Section: Discussionmentioning

confidence: 99%

Me2SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars

et al. 2019

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Cryopreservation is the universal technology used to enable long-term storage and continuous availability of cell stocks and tissues for regenerative medicine demands. The main components of standard freezing media are dimethyl sulfoxide (hereinafter Me 2 SO) and fetal bovine serum (FBS). However, for manufacturing of cells and tissue-engineered products in accordance with the principles of Good Manufacturing Practice (GMP), current considerations in regenerative medicine suggest development of Me 2 SO-and serum-free biopreservation strategies due to safety concerns over Me 2 SO-induced side effects and immunogenicity of animal serum.In this work, the effect of electroporation-assisted pre-freeze delivery of sucrose, trehalose and raffinose into human umbilical cord mesenchymal stem cells (hUCMSCs) on their post-thaw survival was investigated. The optimal strength of electric field at 8 pulses with 100 μs duration and 1 Hz pulse repetition frequency was determined to be 1.5 kV/cm from permeabilization (propidium iodide uptake) vs. cell recovery data (resazurin reduction assay).Using sugars as sole cryoprotectants with electroporation, concentration-dependent increase in cell survival was observed. Irrespective of sugar type, the highest cell survival (up to 80%) was achieved at 400 mM extracellular concentration and electroporation. Cell freezing without electroporation yielded significantly lower survival rates. In the optimal scenario, cells were able to attach 24 h after thawing demonstrating characteristic shape and sugar-loaded vacuoles. Application of 10% Me 2 SO/90% FBS as a positive control provided cell survival exceeding 90%. Next, high glass transition temperatures determined for optimal concentrations of sugars by differential scanning calorimetry (DSC) suggest the possibility to store samples at À 80 � C. In summary, using electroporation to incorporate cryoprotective sugars into cells is an effective strategy towards Me 2 SO-and serumfree cryopreservation and may pave the way for further progress in establishing clinically safe biopreservation strategies for efficient long-term biobanking of cells.

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Effects of trehalose vitrification and artificial oocyte activation on the development competence of human immature oocytes

Cited by 27 publications

References 45 publications

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Predict Value of Relative Telomere Length, TERT, and Follicular Fluid Anti-mullerian Hormone Levels for IVF Outcome in Infertile Women

Comparison of Human Oocyte Activation Between Round-Headed Sperm Injection Followed by Calcium Ionophore Treatment and Normal Sperm Injection in a Patient With Globozoospermia

Me2SO- and serum-free cryopreservation of human umbilical cord mesenchymal stem cells using electroporation-assisted delivery of sugars

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