Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen1

Hu, Jianhong; Liu, Zan; Zhao, Xin; Li, Q.-W.; Jiang, Zhongliang; Li, Y.-K.; Li, X.

doi:10.2527/jas.2009-2335

Cited by 82 publications

(57 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There was significant reduction in sperm motility from initial dilution to equilibration and from equilibration time to post freeze thawing of semen samples in all the four extenders such as control (T0), T50, T100 Aboagla and Terada (2003) reported that freezing of goat sperm at 375 mMtrehalose resulted in significantly greater post-thaw total motility and progressive motility than in the absence of trehalose. Hu et al (2010) did not found any difference between 50 and 100 mM trehalose supplementation on sperm motility of frozen-thawed ram and bull sperm. Woelders et al (1997) demonstrated that an isotonic sugar medium in which Tris-citrate components were substituted with sucrose and trehalose was superior to a Tris-citrate-egg yolk extender for preserving the motility of bovine spermatozoa.…”

Section: Resultsmentioning

confidence: 59%

Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen

Karunakaran

Konyak

Mandal

et al. 2018

IJAR

View full text Add to dashboard Cite

The experiment was conducted to study the effect of trehalose an impermeant extra cellular cryoprotectant supplementation on cryopreservation of Black Bengal buck semen. Semen ejaculates (n = 24) were diluted in Tris-soybean lecithinglycerol extender having trehalose @ 0, 50,100and 150 mM. Samples were equilibrated at 5°C for 3 hrs, frozen and stored in liquid nitrogen. Semen samples were evaluated for sperm motility,functional membrane integrityand concentration of lipid peroxide compound malondialdehyde at three stages such as 10 minsafter dilution with extender, after completion of equilibration period and after freeze thawing. It was found that the control group (0mM trehalose)had significantly better in vitro sperm characters when compared to extenders supplemented with trehalose (50 mM , 100 mM and 150 mM) during different stages of cryopreservation. It can be concluded that supplementation of trehaloseas a cryoprotectant did not improve the cryopreservability of Black Bengal buck semen.

show abstract

Section: Resultsmentioning

confidence: 59%

Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen

Karunakaran

Konyak

Mandal

et al. 2018

IJAR

View full text Add to dashboard Cite

show abstract

“…Trehalose, a small disaccharide molecule of glucose, is considered safe to consume [11] and possesses an exceptional ability to stabilize the biomembrane and preserve cells and tissues during the freeze-thaw procedures [12]. Subsequently, a number of studies have shown that trehalose can be used as an excellent alternative cryoprotectant in the cryopreservation of red blood and germ cells [13][14][15]. Another advantage of trehalose, as previously demonstrated, is its ability to inhibit the apoptosis level [16,17].…”

Section: Introductionmentioning

confidence: 99%

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Lan

Zhao

et al. 2015

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The study aims to assess the protective effects of dimethyl sulfoxide (DMSO)-free solution based on trehalose on the cryopreservation of a whole sheep ovary and evaluate its use as an efficient cryoprotectant. Method Twenty-one ovaries collected from 6-to 8-month-old non-pregnant female sheep were randomly distributed into three groups, namely, a fresh group, a DMSO-free group, and a DMSO group. The morphology, cell apoptosis (by hematoxylin and eosin (HE) staining and terminal deoxynucleotidyltransferasemediated dUTP nick-end labeling (TUNEL) assay), and mRNA transcript of Bcl-2-associated X protein (BAX) and cold inducible RNA-binding protein (CIRP) (by real-time PCR) of the thawed sheep ovaries and fresh controls were tested to establish a criterion for appraising the results of the cryopreservation.Results (i) The histological assessment indicated that the structure of the DMSO-free ovaries remained largely intact and comparable to those of the fresh control groups; whereas, significant damage was observed in the ovaries of the DMSO group (P<0.05). (ii) The TUNEL assay and mRNA transcript of the BAX assessment showed that the apoptosis parameter in the fresh group was the lowest among all the groups (P<0.05), and the parameter in the DMSO-free group was significantly lower than that in the DMSO group (P<0.05). (iii) The level of the CIRP transcripts increased the most in the DMSO-free group followed by the DMSO group and the fresh control group (P<0.05). Conclusions These results indicate that a DMSO-free cryoprotectant solution, especially a trehalose cryoprotectant, is an efficient cryoprotectant and has a beneficial effect on the cryopreservation of whole sheep ovaries.Capsule These results indicate that a DMSO-free and trehalose-containing cryoprotectant solution has a beneficial effect on the cryopreservation of intact sheep ovaries.Tianqi Du and Lan Chao contributed equally to this work.

show abstract

“…Each pooled ejaculate was split into five equal aliquots and diluted at 37 °C with base extenders containing trehalose (10 and 25 mM), cysteine (2 and 4 mM) or no antioxidant (control), respectively. Specific trehalose and cysteine concentrations were selected based on results obtained from a previous trehalose and cysteine standardisation study with ram and bull semen (Aboagla and Terada 2003;Sariozkan et al 2009;Hu et al 2010;Coyan et al 2011;Jafaroghli et al 2011). The final semen concentration was 4 × 10 8 sperm/ml (single step dilution), in 15-ml polypropylene centrifuge tubes.…”

Section: Methodsmentioning

confidence: 99%

Positive effects of trehalose and cysteine on ram sperm parameters

Güngör¹,

Öztürk²,

Omur³

2017

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The aim of this study was to determine the effects of trehalose and cysteine on sperm motility, viability, mitochondrial activity and acrosome integrity during liquid storage of Merino ram semen. Ejaculates were collected using artificial vaginas from five Merino rams, microscopically evaluated and pooled at 37 °C. The pooled semen samples were diluted in a Tris-based extender, including cysteine (2 mM and 4 mM), trehalose (10 mM and 25 mM) and no antioxidant (control). Diluted semen samples were kept in tubes and cooled from 37 to 5 °C in a cold cabinet, and maintained at 5 °C. Cooled samples were evaluated for sperm motility, viability, mitochondrial activity and acrosome integrity at 0, 24, 48, 72 and 96 h. Extender supplemented with trehalose (10 and 25 mM) and cysteine (2 and 4 mM) led to higher motility in comparison to the control at 24, 48, 72 and 96 h of liquid storage (P < 0.05). Trehalose at the doses of 10 mM, 25 mM and 2 mM cysteine led to higher viability between 24-48-72 h and at 96 h of liquid storage (P < 0.05). Further, 4 mM of cysteine improved sperm viability rates at 24 and 48 h of storage compared to the control group (P < 0.05), and resulted in improved acrosome integrity rates compared to the control group at 72 and 96 h of storage (P < 0.05). Extender supplemented with 10 and 25 mM trehalose at 24 and 72 h and 4 mM cysteine at 24 and 96 h of storage led to higher sperm mitochondrial activity rates when compared to the control group (P < 0.05). In conclusion, the findings of this study show that trehalose and cysteine provided significant protection to ram sperm parameters during liquid storage.

show abstract

Effects of trehalose supplementation on semen quality and oxidative stress variables in frozen-thawed bovine semen1

Cited by 82 publications

References 33 publications

Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen

Effect of trehalose- an impermeant cryoprotectant on cryopreservation of Black Bengal buck semen

Successful cryopreservation of whole sheep ovary by using DMSO-free cryoprotectant

Positive effects of trehalose and cysteine on ram sperm parameters

Contact Info

Product

Resources

About