Effects of treatment with <i>Hypoxis hemerocallidea</i>
 extract on sexual behaviour and reproductive parameters in male rats

Tiya, Sibahle Y; Sewani-Rusike, Constance R; Shauli, Mathulo

doi:10.1111/and.12742

Cited by 7 publications

(3 citation statements)

References 38 publications

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“…Monitoring of organ weights gives information on the general wellbeing of animals (Tsai et al, 2003). H. hemerocallidea had no effect on vital organ (heart, liver, spleen and kidneys) weights of the treated dams and pups exposed in utero when compared to the control groups, thus, H. hemerocallidea had no gross toxic effects on these organs, also supported by our previous study in male rats using similar H. hemerocallidea doses (Tiya et al, 2017).…”

Section: Discussionsupporting

confidence: 68%

In utero exposure to Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall. improves metabolic syndrome parameters in pregnant rats and offspring

Sewani-Rusike¹,

Buso²,

Engwa³

et al. 2021

J Pharm Pharmacogn Res

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Context: Intrauterine and early life environments contribute to adult metabolic phenotype. Use of medicinal plants like Hypoxis hemerocallidea during pregnancy raises the question of whether this species may have epigenetic benefits or detriments due to intrauterine-programming, which is phenotypically expressed in the offspring. Aims: To evaluate the effect of H. hemerocallidea on selected markers of metabolic syndrome on dams and their pups (offspring). Methods: Pregnant female Wistar rats (n=18) were divided into three treatment groups (n=6/group): Ethanol extract of H. hemerocallidea at 150 and 300 mg/kg b.w and the control (distilled water) were administered for 21 days. Body weights were monitored and oral glucose tolerance was determined for dams on day 20 of gestation and for pups 28 days postpartum. Serum total antioxidant capacity (TAC), LDL and HDL were determined 28 days postpartum. Results: H. hemerocallidea had no effect on body and organ weights of the treated dams. Pups born to H. hemerocallidea dams had reduced visceral fat compared to the untreated controls. H. hemerocallidea increased the glucose tolerance of treated dams and their pups compared to untreated controls. H. hemerocallidea extract increased serum HDL in treated dams while it decreased LDL in pups born to treated dams. H. hemerocallidea increased TAC in pups born to treated dams. Conclusions: H. hemerocallidea protected pregnant dams and their pups from insulin resistance, improved lipid profiles, reduced visceral fat accumulation and boosted total antioxidant capacity in pups. These protective effects of H. hemerocallidea in pups may have resultedfrom intrauterine programming during pregnancy.

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Section: Discussionsupporting

confidence: 68%

In utero exposure to Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall. improves metabolic syndrome parameters in pregnant rats and offspring

Sewani-Rusike¹,

Buso²,

Engwa³

et al. 2021

J Pharm Pharmacogn Res

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“…Brain tissue fixed in 10% buffered formalin was subjected to standard procedures for fixing, washing, dehydration and paraffin embedding as previously described by us [ 34 ]. Following fixing of the brain tissue, 5-μm sections were cut and mounted on clean slides.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective effects of Lippia javanica (Burm.F.) Spreng. Herbal tea infusion on Lead-induced oxidative brain damage in Wistar rats

Suleman

Engwa

Shauli

et al. 2022

BMC Complement Med Ther

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Background Though Lippia javanica (Burm.f.) Spreng antioxidant activity has been demonstrated, its effect in protecting the brain from lead (Pb)-induced oxidative damage is unknown. This study investigated the effect of L. javanica against Pb-induced oxidative stress, inflammation, apoptosis and acetylcholinesterase activity in rat’s brain. Methods L. javanica herbal tea infusion was prepared, its phytochemical constituent was revealed by liquid chromatography-Mass spectrometer (LC-MS) and was administered simultaneously with Pb. Four groups of male Wistar rats (n = 5/group) were used: control received distilled water; Pb-acetate group received 50 mg Pb/ Kg bodyweight (bw), treatment group received 50 mg Pb/ Kg Pb-acetate + 5 ml/kg bw L. javanica and L. javanica group received 5 ml/Kg bw of L. javanica tea infusion only. After 6 weeks of treatment, oxidative status, acetylcholinesterase activity, inflammation and apoptosis was assessed in brain tissue which was also histologically examined. Results Mean brain and heart weight was reduced (p < 0.05) while liver and spleen weights were increased (p < 0.05) in Pb exposed animals but were prevented by L. juvanica treatment. Treatment with L. javanica increased (p < 0.05) overall brain antioxidant status (glutathione and superoxide dismutase activities) and reduced lipid peroxidation (p < 0.05) compared to the Pb exposed animals. Pro-inflammatory cytokine tumour necrotic factor-alpha, pro-apoptosis Bax protein and anticholinesterase activity were reduced (p < 0.05) in Pb-L. javanica treated animals compared to the Pb exposed group. Histological examination confirmed neuroprotective effects of L. javanica as evidenced by reduced apoptosis/necrosis and inflammation-induced vacuolization and oedema in the hippocampus. The L. javanica treatment alone had no detrimental effects to the rats. LC-MS analysis revealed L. javanica to be rich in phenolics. Conclusions This study demonstrated that L. javanica, rich in phenolics was effective in reducing Pb-induced brain oxidative stress, inflammation, apoptosis, acetylcholinesterase activity and neuronal damage.

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“…Lung tissue fixed in 10% buffered formalin was subjected to standard procedures for fixing, washing, dehydration and paraffin embedding as previously described by us [ 51 ]. Following that, 5 μm sections of the tissue were cut using a sledge microtome (Microm HM355s) and mounted on clean slides.…”

Section: Methodsmentioning

confidence: 99%

Lippia javanica (Zumbani) herbal tea infusion attenuates allergic airway inflammation via inhibition of Th2 cell activation and suppression of oxidative stress

Mfengu

Shauli

Engwa

et al. 2021

BMC Complement Med Ther

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Background Lippia javanica (lemon bush) is commonly used in the treatment of respiratory ailments, including asthma in southern African countries but there is no scientific evidence to support this claim. This study investigated the anti-inflammatory, antioxidant and anti-asthmatic effects of L. javanica using a rat model of asthma. Methods A 5% w/v L. javanica tea infusion was prepared and characterised by liquid chromatography–mass spectrometer (LC-MS). Animals were intraperitoneally sensitized with ovalbumin (OVA) and subsequently challenged intranasal with OVA on day 15 except the control group. Animals were grouped (n = 5/group) for treatment: unsensitised control, sensitised control, sensitised + prednisolone and sensitised + L. javanica at 50 mg/kg/day and 100 mg/kg/day – equivalent to 1 and 2 cups of tea per day, respectively. After 2 weeks of treatment, bronchoalveolar lavage fluid (BALF) was collected for total and differential white blood cell (WBC) count. Nitric oxide (NO), lipid peroxidation and antioxidants were also assessed in BALF. Ovalbumin specific IgE antibody and inflammatory cytokines: IL-4, IL-5, IL-13 and TNF-alpha were measured in serum. Lung and muscle tissues were histological examined. Results L. javanica was rich in phenolic compounds. OVA sensitisation resulted in development of allergic asthma in rats. L. javanica treatment resulted in a reduction in total WBC count as well as eosinophils, lymphocytes and neutrophils in BALF. L. javanica inhibited Th2-mediated immune response, which was evident by a decrease in serum IgE and inflammatory cytokines: IL-4, IL-5, IL-13 and TNF-α. L. javanica treatment also reduced malondialdehyde (MDA) and NO, and increased superoxide dismutase, glutathione and total antioxidant capacity. Histology showed significant attenuation of lung infiltration of inflammatory cells, alveolar thickening, and bronchiole smooth muscle thickening. Conclusion L. javanica suppressed allergic airway inflammation by reducing Th2-mediated immune response and oxidative stress in OVA-sensitized rats which may be attributed to the presence of phenolic compound in the plant. This finding validates the traditional use of L. javanica in the treatment of respiratory disorders.

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Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductive parameters in male rats

Cited by 7 publications

References 38 publications

In utero exposure to Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall. improves metabolic syndrome parameters in pregnant rats and offspring

In utero exposure to Hypoxis hemerocallidea Fisch., C.A.Mey. & Avé-Lall. improves metabolic syndrome parameters in pregnant rats and offspring

Neuroprotective effects of Lippia javanica (Burm.F.) Spreng. Herbal tea infusion on Lead-induced oxidative brain damage in Wistar rats

Lippia javanica (Zumbani) herbal tea infusion attenuates allergic airway inflammation via inhibition of Th2 cell activation and suppression of oxidative stress

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