“…In lambs the stress associated with pre-slaughter handling (including weaning, pre-slaughter starvation and pre-slaughter rest in the lairage), conditions and duration of transport, as well as season has been studied extensively (Sowińska et al, 2001;Fisher et al, 2005;Ali et al, 2006;Kadim et al, 2006Kadim et al, , 2009Bórnez et al, 2009;Tadich et al, 2009;Miranda-de la Lama et al, 2010;Ekiz et al, 2012a,b). Increased blood cortisol levels in lambs after 75 min (Ekiz et al, 2012a), 2 h (Ali et al, 2006) and 48 h of transport (Tadich et al, 2009) indicate that transport is the most significant stressor during pre-slaughter handling.…”