Effects of Transient Hypoxia versus Prolonged Hypoxia on Satellite Cell Proliferation and Differentiation In Vivo

Jash, Sukanta; Adhya, Samit

doi:10.1155/2015/961307

Cited by 21 publications

(18 citation statements)

References 24 publications

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“…HIF-1a plays an important role in maintaining the quiescence of Pax7 + satellite stem cells and preventing their proliferation and differentiation through inhibition of the WNT pathway (42)(43)(44). To test whether HIF-1a affects the expression levels of WNT target genes in MRL/MpJ mouse MDSPCs, we measured the expression levels of these genes in DMOG-treated MRL/MpJ MDSPCs or cells cultured in hypoxia for 24 h (Fig.…”

Section: Hif-1a Promotes Mdspc Stemness Partly Through Down-regulatiomentioning

confidence: 99%

Hypoxia‐inducible factor 1α (HIF‐1α) is a major determinant in the enhanced function of muscle‐derived progenitors from MRL/MpJ mice

et al. 2019

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Although the mouse strain Murphy Roths Large (MRL/MpJ) possesses high regenerative potential, the mechanism of tissue regeneration, including skeletal muscle, in MRL/MpJ mice after injury is still unclear. Our previous studies have shown that muscle‐derived stem/progenitor cell (MDSPC) function is significantly enhanced in MRL/MpJ mice when compared with MDSPCs isolated from age‐matched wild‐type (WT) mice. Using mass spectrometry–based proteomic analysis, we identified increased expression of hypoxia‐inducible factor (HIF) 1α target genes (expression of glycolytic factors and antioxidants) in sera from MRL/MpJ mice compared with WT mice. Therefore, we hypothesized that HIF‐1α promotes the high muscle healing capacity of MRL/MpJ mice by increasing the potency of MDSPCs. We demonstrated that treating MRL/MpJ MDSPCs with dimethyloxalylglycine and CoCl2 increased the expression of HIF‐1α and target genes, including angiogenic and cell survival genes. We also observed that HIF‐1α activated the expression of paired box (Pax)7 through direct interaction with the Pax7 promoter. Furthermore, we also observed a higher myogenic potential of MDSPCs derived from prolyl hydroxylase (Phd) 3—knockout (Phd3−/−) mice, which displayed higher stability of HIF‐1α. Taken together, our findings suggest that HIF‐1α is a major determinant in the increased MDSPC function of MRL/MpJ mice through enhancement of cell survival, proliferation, and myogenic differentiation.—Sinha, K. M., Tseng, C., Guo, P., Lu, A., Pan, H., Gao, X., Andrews, R., Eltzschig, H., Huard, J. Hypoxia‐inducible factor 1α (HIF‐1α) is a major determinant in the enhanced function of muscle‐derived progenitors from MRL/MpJ mice. FASEB J. 33, 8321–8334 (2019). http://www.fasebj.org

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Section: Hif-1a Promotes Mdspc Stemness Partly Through Down-regulatiomentioning

confidence: 99%

Hypoxia‐inducible factor 1α (HIF‐1α) is a major determinant in the enhanced function of muscle‐derived progenitors from MRL/MpJ mice

et al. 2019

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“…A recent study proposed another approach to induce either transient or prolonged hypoxia during skeletal muscle regeneration in rats (47). Six days after injury of the quadriceps muscle caused by a needle, a cocktail of mitochondrion‐targeted RNAs was injected at the injury site.…”

Section: Skeletal Muscle Regeneration and Effects Of Low O2 Levelsmentioning

confidence: 99%

“…Additional experiments are needed to determine to which extent the injured fibers are exposed to low O 2 levels in this model under normoxic conditions. A recent study proposed another approach to induce either transient or prolonged hypoxia during skeletal muscle regeneration in rats (47). Six days after injury of the quadriceps muscle caused by a needle, a cocktail of mitochondrion-targeted RNAs was injected at the injury site.…”

Section: Effects Of Low O 2 Levels On the Regenerative Responsementioning

confidence: 99%

Regulation of myogenesis and skeletal muscle regeneration: effects of oxygen levels on satellite cell activity

Chaillou

Lanner

2016

FASEB j.

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Reduced oxygen (O) levels (hypoxia) are present during embryogenesis and exposure to altitude and in pathologic conditions. During embryogenesis, myogenic progenitor cells reside in a hypoxic microenvironment, which may regulate their activity. Satellite cells are myogenic progenitor cells localized in a local environment, suggesting that the O level could affect their activity during muscle regeneration. In this review, we present the idea that O levels regulate myogenesis and muscle regeneration, we elucidate the molecular mechanisms underlying myogenesis and muscle regeneration in hypoxia and depict therapeutic strategies using changes in O levels to promote muscle regeneration. Severe hypoxia (≤1% O) appears detrimental for myogenic differentiation in vitro, whereas a 3-6% O level could promote myogenesis. Hypoxia impairs the regenerative capacity of injured muscles. Although it remains to be explored, hypoxia may contribute to the muscle damage observed in patients with pathologies associated with hypoxia (chronic obstructive pulmonary disease, and peripheral arterial disease). Hypoxia affects satellite cell activity and myogenesis through mechanisms dependent and independent of hypoxia-inducible factor-1α. Finally, hyperbaric oxygen therapy and transplantation of hypoxia-conditioned myoblasts are beneficial procedures to enhance muscle regeneration in animals. These therapies may be clinically relevant to treatment of patients with severe muscle damage.-Chaillou, T. Lanner, J. T. Regulation of myogenesis and skeletal muscle regeneration: effects of oxygen levels on satellite cell activity.

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“…Additionally, analysis of the oxygen uptake of stem cells revealed shifts in their energy metabolism. These shifts were correlated with the phenotypic changes observed in the study of inherited diseases , wound healing , and differentiation pathways . Therefore, the capacity to monitor the DO in a culture and to quantify the oxygen kinetics of the cells, such as the specific oxygen uptake rates (sOUR), is critical to the development of stem cell‐based therapies.…”

Section: Introductionmentioning

confidence: 99%

Real‐time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device

Super

Jaccard

Marques

et al. 2016

Biotechnology Journal

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Oxygen plays a key role in stem cell biology as a signaling molecule and as an indicator of cell energy metabolism. Quantification of cellular oxygen kinetics, i.e. the determination of specific oxygen uptake rates (sOURs), is routinely used to understand metabolic shifts. However current methods to determine sOUR in adherent cell cultures rely on cell sampling, which impacts on cellular phenotype. We present real‐time monitoring of cell growth from phase contrast microscopy images, and of respiration using optical sensors for dissolved oxygen. Time‐course data for bulk and peri‐cellular oxygen concentrations obtained for Chinese hamster ovary (CHO) and mouse embryonic stem cell (mESCs) cultures successfully demonstrated this non‐invasive and label‐free approach. Additionally, we confirmed non‐invasive detection of cellular responses to rapidly changing culture conditions by exposing the cells to mitochondrial inhibiting and uncoupling agents. For the CHO and mESCs, sOUR values between 8 and 60 amol cell−1 s−1, and 5 and 35 amol cell−1 s−1 were obtained, respectively. These values compare favorably with literature data. The capability to monitor oxygen tensions, cell growth, and sOUR, of adherent stem cell cultures, non‐invasively and in real time, will be of significant benefit for future studies in stem cell biology and stem cell‐based therapies.

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Effects of Transient Hypoxia versus Prolonged Hypoxia on Satellite Cell Proliferation and Differentiation In Vivo

Cited by 21 publications

References 24 publications

Hypoxia‐inducible factor 1α (HIF‐1α) is a major determinant in the enhanced function of muscle‐derived progenitors from MRL/MpJ mice

Hypoxia‐inducible factor 1α (HIF‐1α) is a major determinant in the enhanced function of muscle‐derived progenitors from MRL/MpJ mice

Regulation of myogenesis and skeletal muscle regeneration: effects of oxygen levels on satellite cell activity

Real‐time monitoring of specific oxygen uptake rates of embryonic stem cells in a microfluidic cell culture device

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