Effects of Transgenic &lt;I&gt;Bacillus thuringiensis&lt;/I&gt; Maize Grain on &lt;I&gt;B. thuringiensis&lt;/I&gt;-Susceptible &lt;I&gt;Plodia interpunctella&lt;/I&gt; (Lepidoptera: Pyralidae)

Hellmich, Richard L.; Iverson, Cort T.; Lewis, Leslie C.

doi:10.1603/0022-0493-93.3.1011

Cited by 30 publications

(27 citation statements)

References 18 publications

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“…observed with P33V08 and N6800Bt hybrids. These data are in accord with those obtained by Giles et al (2000) who examined survival, development, and body size of P. interpunctella developing on several transgenic Bt hybrids. Angoumois grain moth showed a slight but signiÞcant delay in development with MON 810, but there was no difference with Bt-11 or other Bt lines.…”

Section: Discussionsupporting

confidence: 90%

“…Adults were not sexed to minimize handling and potentially affecting survival and fecundity. A 1:1 male:female sex ratio was assumed based on previous colony performance and on sex ratio not being skewed by transgenic Bt grain as reported by Giles et al (2000). Indian meal moth and Angoumois grain moth eggs were counted at weekly intervals for 5 wk after introducing adults.…”

Section: Methodsmentioning

confidence: 99%

“…Giles et al (2000) examined P. interpunctella development, survivorship, sex ratio, and adult body length and found that some Bt corn grain can negatively impact Bt susceptible P. interpunctella populations. Additional stored-product pest species and other life history attributes such as fecundity also need to be investigated.…”

mentioning

confidence: 99%

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Life History Attributes of Indian Meal Moth (Lepidoptera: Pyralidae) and Angoumois Grain Moth (Lepidoptera: Gelechiidae) Reared on Transgenic Corn Kernels

Sedlacek¹,

Komaravalli²,

Hanley³

et al. 2001

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The Indian meal moth, Plodia interpunctella (Hübner), and Angoumois grain moth, Sitotroga cerealella (Olivier), are two globally distributed stored-grain pests. Laboratory experiments were conducted to examine the impact that corn (Zea mays L.) kernels (i.e., grain) of some Bacillus thuringiensis Berliner (Bt) corn hybrids containing CrylAb Bt delta-endotoxin have on life history attributes of Indian meal moth and Angoumois grain moth. Stored grain is at risk to damage from Indian meal moth and Angoumois grain moth; therefore, Bt corn may provide a means of protecting this commodity from damage. Thus, the objective of this research was to quantify the effects of transgenic corn seed containing CrylAb delta-endotoxin on Indian meal moth and Angoumois grain moth survival, fecundity, and duration of development. Experiments with Bt grain, non-Bt isolines, and non-Bt grain were conducted in environmental chambers at 27 +/- 1 degrees C and > or = 60% RH in continuous dark. Fifty eggs were placed in ventilated pint jars containing 170 g of cracked or whole corn for the Indian meal moth and Angoumois grain moth, respectively. Emergence and fecundity were observed for 5 wk. Emergence and fecundity of Indian meal moth and emergence of Angoumois grain moth were significantly lower for individuals reared on P33V08 and N6800Bt, MON 810 and Bt-11 transformed hybrids, respectively, than on their non-Bt transformed isolines. Longer developmental times were observed for Indian meal moth reared on P33V08 and N6800Bt than their non-Bt-transformed isolines. These results indicate that MON 810 and Bt-11 CrylAb delta-endotoxin-containing kernels reduce laboratory populations of Indian meal moth and Angoumois grain moth. Thus, storing Bt-transformed grain is a management tactic that warrants bin scale testing and may effectively reduce Indian meal moth and Angoumois grain moth populations in grain without application of synthetic chemicals or pesticides.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

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Life History Attributes of Indian Meal Moth (Lepidoptera: Pyralidae) and Angoumois Grain Moth (Lepidoptera: Gelechiidae) Reared on Transgenic Corn Kernels

Sedlacek¹,

Komaravalli²,

Hanley³

et al. 2001

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“…Transgenic cotton plants expressing Bt genes provide excellent protection against lepidopteran insects throughout the season and significantly higher yields in the presence of such pests (Artim 2003). Benefits of transgenic, insect-resistant crops include reduced applications of broad spectrum insecticides (Carpenter et al 2002), increased protection due to season-long control of the target insect pests (Rice and Pilcher 1998), protection of stored grain from insect pests (Giles et al 2000), and lower mycotoxin levels due to reduction of fungal pathogens associated with insect damage (Munkvold et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Expression and inheritance of chimeric cry2AX1 gene in transgenic cotton plants generated through somatic embryogenesis

Sakthi

Naveenkumar

Deepikha

et al. 2015

In Vitro Cell.Dev.Biol.-Plant

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Transgenic cotton plants expressing a novel cry2AX1 gene consisting of sequences from the cry2Aa and cry2Ac genes, driven by the CaMV35S or the EnCaMV35S promoters, were generated through Agrobacterium-mediated transformation. Among 40 independent PCR-positive events, 14 produced Cry2AX1 protein at detectable levels. Cry2AX1 levels in T 0 plants ranged from 0.012 to 1.068 μg/g fresh leaf tissue. Insect bioassays on Cry2AX1-expressing T 0 transgenic lines showed 27.0 to 88.8% mortality of Helicoverpa armigera. Southern hybridization analysis revealed single locus integration of the cry2AX1 gene in two T 0 plants which caused 87.5 and 88.8% mortality in H. armigera. The presence and expression of the cry2AX1 gene were confirmed in T 1 progeny using PCR and quantitative ELISA. The highest expression level of Cry2AX1 protein recorded in the T 1 generation was 0.766 μg/g fresh leaf tissue, far less than the expression of Cry2Ab protein (23 μg/g leaf tissue) reported in the commercialized Bt cotton, Bollgard II. In spite of the low Cry2AX1 level, the mortality against H. armigera ranged between 33.3 and 85.7% in the T 1 generation. These results indicate that this novel cry2AX1 gene produced a potent insecticidal protein that was effective against H. armigera in transgenic cotton plants at very low concentrations.

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“…Previous studies have shown that emergence and fecundity in two species of stored-product moths, P. interpunctella and S. cerealella, had declined when reared on lepidopteran-specific Bt transgenic maize. In P. interpunctella, this effect can last up to 5 months after harvest (Sedlacek et al, 2001;Giles et al, 2000). In addition, the development time from egg to adult for both sexes had increased when reared on Bt maize (Gryspeirt and Grégoire, 2012).…”

mentioning

confidence: 99%

Transgenic cry1C⁎ gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae)

Sun

Yan

Zhang

et al. 2015

Ecotoxicology and Environmental Safety

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Effects of Transgenic <I>Bacillus thuringiensis</I> Maize Grain on <I>B. thuringiensis</I>-Susceptible <I>Plodia interpunctella</I> (Lepidoptera: Pyralidae)

Cited by 30 publications

References 18 publications

Life History Attributes of Indian Meal Moth (Lepidoptera: Pyralidae) and Angoumois Grain Moth (Lepidoptera: Gelechiidae) Reared on Transgenic Corn Kernels

Life History Attributes of Indian Meal Moth (Lepidoptera: Pyralidae) and Angoumois Grain Moth (Lepidoptera: Gelechiidae) Reared on Transgenic Corn Kernels

Expression and inheritance of chimeric cry2AX1 gene in transgenic cotton plants generated through somatic embryogenesis

Transgenic cry1C⁎ gene rough rice line T1C-19 does not change the host preferences of the non-target stored product pest, Rhyzopertha dominica (Fabricius) (Coleoptera: Bostrichidae), and its parasitoid wasp, Anisopteromalus calandrae (Howard) (Hymenoptera: Pteromalidae)

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