Effects of transforming growth factor-β deficiency on bone development: A Fourier Transform-Infrared imaging analysis

Atti, Elisa; Gómez, Santiago; Wahl, Sharon M.; Mendelsohn, Richard; Paschalis, E. P.; Boskey, Adele L.

doi:10.1016/s8756-3282(02)00905-5

Cited by 54 publications

(40 citation statements)

References 51 publications

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“…In the TGF 1-null metaphyses, there was a persistence of trabeculae, but no significant alterations in mineral content or crystallinity. Mineral content, mineral crystallinity, and collagen maturity were reduced in the secondary ossification center and cortical bone of the TGF-1 null mice, all consistent with a mechanism of impaired bone maturation in the TGF-1 null mice, were suggested to be directly related to TGF-1 deficiency and indirectly related to increased expression of other inflammatory cytokines in the TGF 1-null mice [261]. Histomorphometric and radiographic analyses of TGF 1-null mice demonstrated similar features, however, it was noted that the width of the growth plate in the tibia and the longitudinal growth rate were significantly decreased in the TGF 1-null mice.…”

Section: Growth Factors Cytokines and Enzymes Relevant To Mineralizsupporting

confidence: 52%

Mechanism of Mineralization of Collagen‐Based Connective Tissues

Boskey

2008

Biomineralization

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Section: Growth Factors Cytokines and Enzymes Relevant To Mineralizsupporting

confidence: 52%

Mechanism of Mineralization of Collagen‐Based Connective Tissues

Boskey

2008

Biomineralization

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“…The models [35][36][37][38][39][40][41][42][43][44][45][46][47] analyzed by point-by-point FT-IR microspectroscopy and Imaging are listed in Table II. Information on wildtype mice from these studies, while varying in absolute values depending on the background strain, confirms general trends noted in human and other animal models.…”

Section: Age Dependent Changes In Ft-ir Spectral Parametersmentioning

confidence: 99%

FT-IR imaging of native and tissue-engineered bone and cartilage

2007

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Fourier transform Infrared (FT-IR) imaging and microspectroscopy have been extensively applied to the analyses of tissues in health and disease. Spatially resolved mid-infrared data has provided insights into molecular changes that occur in diseases of connective or collagen-based tissues, including osteoarthritis, osteoporosis, osteogenesis imperfecta, osteopetrosis and pathologic calcifications. These techniques have also been used to probe chemical changes associated with load, disuse, and micro-damage in bone, and with degradation and repair in cartilage. This review summarizes the applications of FT-IR microscopy and imaging for analyses of bone and cartilage in healthy and diseased tissues, and illustrates the application of these techniques for the characterization of tissue engineered bone and cartilage.

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“…FTIRI compositional parameters that are associated with bone quality include: (1) the mineral-to-matrix ratio, which corresponds to the ash weight of bone [Pienkowski et al, 1997]; (2) the carbonate-to-phosphate ratio, which represents the carbonate portion of the mineral content; (3) crystallinity, which correlates to the size and perfection in the c-axis direction as determined by X-ray diffraction [Gadaleta et al, 1996], and (4) the collagen crosslink ratio which is related to the maturity of the collagen [Atti et al, 2002;Paschalis et al, 2001]. Images were collected for 3 randomly selected trabeculae in 3 sections per animal (n = 9).…”

Section: Methodsmentioning

confidence: 99%

Fourier Transform Infrared Imaging Analysis of Cancellous Bone in Alendronate- and Raloxifene-Treated Osteopenic Sheep

Calton

MacLeay²,

Boskey³

2011

Cells Tissues Organs

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Fourier transform infrared imaging spectroscopy (FTIRI)-assessed bone composition parameters (mineral content, collagen maturity, crystal size and perfection, and carbonate content) describe bone quality and correlate to bone fracture risk. The challenge with studying bone quality in patients treated with antiresorptive drugs such as bisphosphonates (e.g., alendronate) and selective estrogen receptor modulators (SERMs) (e.g. raloxifene) is being able to test bone mechanical performance and material properties pre- and posttreatment. The purpose of this study was to evaluate the FTIRI changes in a large animal model of osteoporosis (female sheep with dietary induced metabolic acidosis; MA). Previous studies have investigated the relationship between bone material properties and bone strength in humans and smaller animals and have shown that changes in compositional properties influence fracture risk. Here we characterize the MA model at 6 and 12 months, demonstrate the loss of bone and changes in compositional properties, and show that 6 months of treatment with both antiresorptives ameliorate the bone loss as assessed by bone mineral density and FTIRI. This preliminary data suggest that the MA sheep model allows investigation of whether drug treatments preserve bone properties that exist at the time of treatment or if they induce further beneficial changes.

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Effects of transforming growth factor-β deficiency on bone development: A Fourier Transform-Infrared imaging analysis

Cited by 54 publications

References 51 publications

Mechanism of Mineralization of Collagen‐Based Connective Tissues

Mechanism of Mineralization of Collagen‐Based Connective Tissues

FT-IR imaging of native and tissue-engineered bone and cartilage

Fourier Transform Infrared Imaging Analysis of Cancellous Bone in Alendronate- and Raloxifene-Treated Osteopenic Sheep

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