Effects of Tranexamic acid and its derivatives on the metabolism of Glutathione/Thiols in freshly isolated Leukocytes

Khan, Amer Hayat; Amir, Faheem; Arjun, Mallik; Khan, Jia-u-din; Khan, Nasir Hayat

doi:10.4103/2045-080x.112811

Cited by 1 publication

(1 citation statement)

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“…According to Japanese regulations regarding products containing TA, a cosmetic product is considered safe as a whitening agent if its TA concentration does not exceed 1.5-2% [8]. TA can also cause severe irritation and allergies under skin conditions that are sensitive to the agent [9][10][11]. TA does not have a high number of chromophore groups, and thus it is difficult to detect through UV spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

Development of a Direct Method of Analyzing Tranexamic Acid Levels in Whitening Cream Using Reversed Phase High-Performance Liquid Chromatography

2020

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Objective: Whitening cream is a cosmetic that contains ingredients that can alleviate hyperpigmentation. Tranexamic acid (TA) is one of the potentialanti-pigmentation agents that work through inhibiting plasmin. TA is used in cosmetic formulations at a concentration of 2.5% as a whitening andmoisturizing agent. To date, research on TA in both cosmetics and other pharmaceutical products using high-performance liquid chromatography(HPLC) has not been done directly (without derivatization). Therefore, this study aimed to develop a simple and rapid analytical method for TA(without derivatization) in cosmetic cream samples using reverse-phase HPLC and water as a solvent.Methods: Optimization was conducted by evaluating several parameters that affect sample extraction, as well as composition and mobile phasetypes. The optimal method must fulfill suitability and validation requirements. The optimal method should be able to detect and quantify TA in creamsamples without derivatization.Results: The optimal analysis condition used a ultraviolet detector at a wavelength of 210 nm, acetonitrile: double-distilled water: phosphoric acid(64:34:2) as the mobile phase and a flow rate of 0.8 mL/min. The retention time of the analyte occurred in the 2nd min.Conclusion: The analytical method that met the validation requirements was characterized using parameters such as accuracy, precision, linearity,selectivity, limit, of detection, and limit of quantitation. This method is applicable for analyzing TA content in samples with a concentration of 1.02%.

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Section: Introductionmentioning

confidence: 99%