Effects of Traditional Chinese Medicinal Plants on Anti-insulin Resistance Bioactivity of DXMS-Induced Insulin Resistant HepG2 Cells

Ma, Jun-Zeng; Yang, L.; Shen, Xiaoling; Qin, Ji-Huan; Deng, Ling; Ahmed, Selena; Xu, Hong‐Xi; Xue, Dayuan; Ye, Jiangxia; Xu, Gang

doi:10.1007/s13659-014-0028-0

Cited by 10 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effects of QJJ on HepG2 cell viability and glucose uptake suggest that it can attenuate the apoptosis and necrosis of HepG2 cells and increase the glucose uptake, exerting protective effect on IR. This is in contrast to a study by Ma et al [ 30 ] in which Astragalus membranaceus was ineffective at increasing insulin sensitivity of dexamethasone-induced insulin resistant HepG2 cells. The inconsistencies may be due to the in vitro IR model employed as APS improved glucose uptake by C2C12 skeletal muscle myotubes after induction of palmitate-induced IR [ 31 ], and Rehmannia increased glucose uptake by insulin resistant HepG2 cells [ 22 ].…”

Section: Discussioncontrasting

confidence: 99%

Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

Zhang

Yu²,

Liu³

et al. 2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

We analyzed the effects of a traditional Chinese medicine, Qizhi Jiangtang Jiaonang (QJJ), on insulin resistance (IR) in vitro. After an in vitro model of IR was established by treating human liver cancer cells (HepG2 cells) with palmitic acid, the cells were then treated with various concentrations of QJJ. Treatment with 400 µM palmitic acid for 24 h induced IR in HepG2 cells. The survival rate for HepG2 cells in the IR group was significantly lower than that of the untreated control group (P < 0.001); however, QJJ restored HepG2 cell survival (P < 0.001). As compared with HepG2 cells in the IR group, QJJ at all doses analyzed significantly increased glucose consumption (all P < 0.05). Moreover, treatment with all the QJJ doses significantly reduced the mean intracellular reactive oxygen species levels as compared with the IR group (all P < 0.05). Furthermore, high-dose QJJ reduced both TNF-α and IL-6 levels as compared to the IR group (all P < 0.05). QJJ ameliorated the altered PI3K, GLUT4, and RAGE expression observed with IR. In conclusion, QJJ can improve IR in HepG2 cells, which may be mediated through the IRS-1/PI3K/GLUT4 signaling pathway as well as regulation of NF-κB-mediated inflammation and oxidative stress.

show abstract

Section: Discussioncontrasting

confidence: 99%

Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

Zhang

Yu²,

Liu³

et al. 2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…This makes them an ideal cell model for studying IR. [43][44][45] This study, which is consistent with previous studies, revealed that PA reduced uptake of a glucose molecule and glycogen synthesis in HepG2 cells to a significant extent, indicating that the cell model of IR was successfully established. 4,7) QAG, a flavonoid isolated from EUOL, increased uptake of glucose as well as glycogen synthesis in PA-induced HepG2cells, thus improving IR.…”

Section: Discussionsupporting

confidence: 91%

Quercetin-3-O-α-L-arabinopyranosyl-(1→2)-β-D-glucopyranoside Isolated from Eucommia ulmoides Leaf Relieves Insulin Resistance in HepG2 Cells via the IRS-1/PI3K/Akt/GSK-3β Pathway

Tang

Liu

et al. 2023

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

For nearly 2000 years, Eucommia ulmoides Oliver (EUO) has been utilized in traditional Chinese medicine (TCM) throughout China. Flavonoids present in bark and leaves of EUO are responsible for their antioxidant, anti-inflammatory, antitumor, anti-osteoporosis, hypoglycemic, hypolipidemic, antibacterial, and antiviral properties, but the main bioactive compound has not been established yet. In this study, we isolated and identified quercetin glycoside (QAG) from EUO leaves (EUOL) and preliminarily explored its molecular mechanism in improving insulin resistance (IR). The results showed that QAG increased uptake of glucose as well as glycogen production in the palmitic acid (PA)-induced HepG2 cells in a dose-dependent way. Further, we observed that QAG increases glucose transporters 2 and 4 (GLUT2 and GLUT4) expression and suppresses the phosphorylation of insulin receptor substrate (IRS)-1 at serine 612 , thus promoting the expression of phosphatidylinositol-3-kinase (PI3K) at tyrosine 458 and tyrosine 199 , as well as protein kinase B (Akt) and glycogen synthase kinase (GSK)-3β at serine 473 and serine 9 , respectively. The influence posed by QAG on the improvement of uptake of glucose was significantly inhibited by LY294002, a PI3K inhibitor. In addition, the molecular docking result showed that QAG could bind to insulin receptors. In summary, our data established that QAG improved IR as demonstrated by the increased uptake of glucose and glycogen production through a signaling pathway called IRS-1/PI3K/Akt/GSK-3β.

show abstract

“…There are many methods for establishing an insulin resistance model in hepatocytes in vitro, such as high glucose induction,8 insulin induction,9 high glucose combined with insulin induction,10 PA induction,11 and dexamethasone induction 12. Here, we used 0.25 mmol/L PA treatment, which is the concentration chosen in most studies5 and in our previous study.…”

Section: Discussionmentioning

confidence: 99%

Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4

Zhao¹,

Shu²,

Huang³

et al. 2019

DMSO

View full text Add to dashboard Cite

Purpose The aim of this study was to determine whether resveratrol (Rev) affects the expression, phosphorylation, and nuclear and cytoplasmic distribution of histone deacetylase 4 (HDAC4), which in turn affects gluconeogenesis in hepatocytes under an insulin-resistant state. Materials and methods HepG2 cells were treated with 0.25 mmol/L palmitic acid (PA) to establish an insulin resistance model. The cells were divided into five groups: control, PA, PA + Rev 100 µM, PA + Rev 50 µM, and PA + Rev 20 µM. After treatment for 24 hours, mRNA and protein expression levels of gluconeogenesis pathway-related molecules and HDAC4 were examined. Next, HepG2 cells were transfected with siRNA-HDAC4. The cells were divided into control, PA, PA + Rev 20 µM, PA + Rev 20 µM +siRNA-HDAC4 negative control, and PA + Rev 20 µM +siRNA-HDAC4 knockdown groups to determine the expression of gluconeogenesis pathway proteins. Results Compared with the control group, the gluconeogenesis pathway-related molecules, glucose-6-phosphatase catalytic subunit (G6PC), phosphoenolpyruvate carboxykinase 1 (PCK1) and forkhead box protein O1 (FOXO1), were increased, and the phosphorylation of FOXO1 decreased after PA treatment. The p-HDAC4 level decreased with the increase in HDAC4 in the nucleus and the decrease in HDAC4 in the cytoplasm in the PA group. Treatment with Rev 20 µM suppressed gluconeogenesis and promoted HDAC4 shuttling into the cytoplasm from the nucleus. However, 100 and Rev 50 µM exerted the opposite effects. Finally, after HDAC4 knockdown, the expression levels of the key gluconeogenesis molecules, G6PC, PCK1, and FOXO1, were increased, and p-FOXO1 was decreased, indicating that gluconeogenesis was enhanced. Conclusion A low concentration of Rev inhibited gluconeogenesis under insulin-resistance conditions via translocation of HDAC4 from the nucleus to the cytoplasm.

show abstract

Effects of Traditional Chinese Medicinal Plants on Anti-insulin Resistance Bioactivity of DXMS-Induced Insulin Resistant HepG2 Cells

Cited by 10 publications

References 37 publications

Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

Qizhi Jiangtang Jiaonang Improves Insulin Signaling and Reduces Inflammatory Cytokine Secretion and Reactive Oxygen Species Formation in Insulin Resistant HepG2 Cells

Quercetin-3-<i>O</i>-α-L-arabinopyranosyl-(1→2)-β-D-glucopyranoside Isolated from <i>Eucommia ulmoides</i> Leaf Relieves Insulin Resistance in HepG2 Cells <i>via</i> the IRS-1/PI3K/Akt/GSK-3β Pathway

<p>Resveratrol affects hepatic gluconeogenesis via histone deacetylase 4</p>

Contact Info

Product

Resources

About