Effects of Topical Applications of Epidermal Growth Factor on Wound Healing Experimental Study on Rabbit Ears

Franklin, John D.; Lynch, John B.

doi:10.1097/00006534-197912000-00003

Cited by 85 publications

(35 citation statements)

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“…The biologic activity ofpurified bFGF toward keratinocytes in vitro was only recently reported (31 (12,34,35). In this work, EGF promoted reepithelialization with a single application and thus this model may be a highly appropriate model to further analyze EGF as a wound healing agent.…”

Section: Resultsmentioning

confidence: 71%

Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model.

Mustoe¹,

Pierce²,

Morishima³

et al. 1991

J. Clin. Invest.

321

218

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The roles of polypeptide growth factors in promoting wound healing and in directing the specificity and sequence of responses of different tissues in wounds are little understood. We investigated the influence of four growth factors on the rates of healing of a novel full thickness dermal ulcer placed on an avascular base in the rabbit ear. The wound model precludes significant wound contraction and requires new granulation tissue and epithelial cells for healing to originate centripetally. 5 ;Ig pmol/mm2) of platelet-derived growth factor-B chain (PDGF-BB), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) applied locally at the time of wounding resulted in a twofold increase in complete reepithelialization of treated wounds (PDGF-BB, P = 0.02 chi square analysis; bFGF, P = 0.04; EGF, P = 0.05); transforming growth factor (TGF)-f}1 significantly inhibited reepithelialization (P = 0.05).Both PDGF-BB and TGF-,f1 uniquely increased the depth and area of new granulation tissue (P < 0.005), the influx of fibroblasts, and the deposition of new matrix into wounds. Explants from 7-d old PDGF-BB-treated wounds remained metabolically far more active than controls, incorporating 473% more [3Hlthymidine into DNA (P = 0.05) and significantly more I3Hlleucine and I3Hlproline into collagenase-sensitive protein (P = 0.04). The results establish that polypeptide growth factors have significant and selective positive influences on healing of full thickness ulcers in the rabbit. (J. Clin. Invest. 1991.

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Section: Resultsmentioning

confidence: 71%

Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model.

Mustoe¹,

Pierce²,

Morishima³

et al. 1991

J. Clin. Invest.

321

218

View full text Add to dashboard Cite

show abstract

“…Although the use of EGF in human impaired wound healing by Falanga et al (1992) showed a modest improvement in wound healing times and rate of epithelialization, the results were not statistically significant. Some other reports also suggested that the efficacy of EGF in chronic wounds is limited (Franklin & Lynch 1979;Brown et al, 1989). Therefore, no conclusions about the efficacy of EGF for chronic wounds can be made at present.…”

Section: Epidermal Growth Factor (Egf)mentioning

confidence: 98%

“…Enhanced wound healing has been noted in dermal wounds treated with topical or subcutaneous EGF (Starkey et al, 1975). Franklin & Lynch (1979) recorded quicker epithelial regeneration and less scar contracture in EGF-treated wounds in rabbits. With the introduction of recombinant human EGF in the 1980s, the range of studies increased to include burn wounds and chronic ulcers (Brown et al, 1989;Falanga et al, 1992).…”

Section: Epidermal Growth Factor (Egf)mentioning

confidence: 98%

Recent Innovation in Pretreatment for Skin Grafts Using Regenerative Medicine in the East

Sano¹,

Ichioka²

2011

Skin Grafts - Indications, Applications and Current Research

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“…In addition, EGF not only promotes formation of granulation tissue but also stimulates fibroblast motility that induces cell-cell adhesive properties consistent with an epithelial phenotype, thereby enhancing the early wound closure [5,10]. Topical application of ointment with EGF to full-thickness wounds made in rabbit's ears enabled the early repair of the wound [8]. Further, application of a silver sulfadiazine cream with EGF has brought significant acceleration of epidermal regeneration at the sites of grafted skins in 12 human patients [2].…”

mentioning

confidence: 99%

Acceleration of Wound Healing by Gelatin Film Dressings with Epidermal Growth Factor

2005

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ABSTRACT. We examined in vivo efficiency of a gelatin film sheet with epidermal growth factor (EGF) for a novel therapeutic device for cutaneous wound repair. NIH3T3 fibroblasts and PAM212 keratinocytes proliferated when the cells were incubated with the homogenate of EGF containing gelatin sheets, indicating that the gelatin sheet retained biologic activity of EGF in the process of sheet formation. To evaluate therapeutic effects, the EGF containing gelatin sheets or control sheets were applied onto partial-thickness skin wounds made on dorsa of hairless dogs. Wound closure in wounds treated with EGF containing gelatin sheets was accelerated when compared to that of wounds treated with control sheets, exhibiting earlier reepithelialization of the epidermis and highly regulated repair of extracellular matrix in the dermis. Therefore, we concluded that the gelatin film is a useful material to keep EGF stable and the EGF containing sheet has the ability to become an efficient therapeutic agent for superficial or deep partial-thickness wounds in the skin.

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Effects of Topical Applications of Epidermal Growth Factor on Wound Healing Experimental Study on Rabbit Ears

Cited by 85 publications

References 0 publications

Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model.

Growth factor-induced acceleration of tissue repair through direct and inductive activities in a rabbit dermal ulcer model.

Recent Innovation in Pretreatment for Skin Grafts Using Regenerative Medicine in the East

Acceleration of Wound Healing by Gelatin Film Dressings with Epidermal Growth Factor

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