Effects of Topical Application of Mitomycin-C and 5-Fluorouracil on Myringotomy in Rats

Cincik, Hakan; Güngör, Atila; Cekin, Engin; Sağlam, Ömer; Yıldırım, Şükrü; Poyrazoğlu, Ethem; Candan, Hasan

doi:10.1097/01.mao.0000169797.60824.3b

Cited by 27 publications

(31 citation statements)

References 18 publications

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“…Mitomycin-C, 5-flourouracil (5-FU), and halofuginone used in myringotomized animal ears were shown to extend the patency of myringotomies [8][9][10][11][12][13]. With mitomycin-C application the mean closure time of myringotomies was between 6.5 and 9.5 weeks in rats [8][9][10][11].…”

Section: Discussionmentioning

confidence: 99%

“…Myringotomy patency was maintained up to two to three weeks with topical 5-FU in animal studies [11,12]. In a clinical study 5-FU ointment applied to patients' myringotomy sites was shown to be effective in prolonging the myringotomy closure time up to four weeks; the average time to myringotomy closure was 20.5 days [30].…”

Section: Discussionmentioning

confidence: 99%

“…Fibrosis was evaluated using a four-point scale comparing the thickness of the tympanic membrane on a qualitative basis: 1) normal tympanic membrane thickness was categorized as "no fibrosis" in the lamina propria; 2) a two-to three-fold increase in tympanic membrane thickness was categorized as "slight fibrosis"; 3) a four-to five-fold increase in tympanic membrane thickness was categorized as "moderate fibrosis"; and 4) a more than five-fold increase in tympanic membrane thickness was categorized as "significant fibrosis" in the lamina propria [12].…”

Section: Histopathologic Examinationmentioning

confidence: 99%

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Extending Myringotomy Patency with Topical Everolimus in Rats

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Histopathologic Examinationmentioning

confidence: 99%

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Extending Myringotomy Patency with Topical Everolimus in Rats

et al. 2016

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“…It is well known that closure is delayed with the application of MMC after TM perforation [8][9][10] . Many studies emphasize the crucial role of growth factors in the wound-healing process [1,2,10] .…”

Section: Discussionmentioning

confidence: 99%

Effect of Mitomycin C on bFGF, TGF-β1, KGF-1 Expressions after Myringotomy: An Animal Study

Durak

Atmaca²,

Karayığıt³

et al. 2016

Int Adv Otol

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OBJECTIVE:The purpose of this study was to evaluate the effect of topical mitomycin C (MMC) on growth factor expression in tympanic membrane (TM) wound healing. MATERIALS and METHODS:Forty (20 male and 20 female) adult Wistar albino rats that varied from 250 to 300 g in weight were divided into five groups. In the first group, no intervention was performed, and the intact TMs were excised after the rats were sacrificed. In the other groups, both ears of rats underwent an electrocautery myringotomy procedure; MMC was applied to the right ears and saline to the left ones. In all groups, on the 3 rd , 7 th , 14 th , and 30 th days, macroscopic examinations of TM patency and the expressions of transforming growth factor-β1 (TGF-β1), basic fibroblast growth factor (bFGF), and keratinocyte growth factor-1 (KGF-1) in TM epithelia, fibroblasts, and macrophages were performed by immunohistochemical staining and compared among the groups. RESULTS:Complete healing was significantly less in the MMC group with respect to the saline group on the 7 th and 14 th days (p<0.05). On immunohistochemical study, no significant differences in the expressions of bFGF or KGF-1 were observed among the groups with one exception; on the 3 rd day, the expression of TGF-β1 in macrophages was more elevated in the MMC group than in the saline group (p=0.001). CONCLUSION:Application of MMC to acute perforations of the TM delays closure and has significant effects on some growth factors for certain durations.

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“…12 MMC has been used in ophthalmological, orthopedic, and otolaryngological surgery due to its antifibrotic effect. [13][14][15] In this study, we aimed to evaluate whether or not MMC has an antifibrotic effect on TGF-β-induced PD in a rat model. To our knowledge, this is the first study evaluating the antifibrotic effect of MMC in PD.…”

Section: Introductionmentioning

confidence: 99%

The histopathological effects of intracavernosal mitomycin-C injection in a rat Peyronie's disease model

Kaya

Kïbar

Yılmaz

et al. 2017

CUAJ

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Introduction: We aimed to evaluate whether or not mitomycin-C (MMC) has an antifibrotic effect on transforming growth factor-beta (TGF-β)-induced Peyronie's disease (PD) in a rat model. Methods: Eighteen 12-week-old male Sprague-Dawley rats were divided into three groups: Group 1=TGF-β1 (n=7); Group 2=TGF-β1+MMC (n=7); and Group 3=Sham group (0.25 ml bovine serum albumin injected) (n=4). All groups were sacrificed on the sixth week of the procedure and their penises were excised. All penis specimens were evaluated semi-quantitatively and quantitatively with histochemical, immunohistochemistry, and image analysis.

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Effects of Topical Application of Mitomycin-C and 5-Fluorouracil on Myringotomy in Rats

Abstract: MMC is more effective than 5-FU, which is more effective than the simple myringotomy procedure in extending the patency of myringotomies in rat tympanic membranes (p < 0.05). Both medications are useful as an adjunct in preventing myringotomy closure.

Cited by 27 publications

References 18 publications

Extending Myringotomy Patency with Topical Everolimus in Rats

Extending Myringotomy Patency with Topical Everolimus in Rats

Effect of Mitomycin C on bFGF, TGF-β1, KGF-1 Expressions after Myringotomy: An Animal Study

The histopathological effects of intracavernosal mitomycin-C injection in a rat Peyronie's disease model

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